FastQCFastQC Report
Tue 23 May 2023
EGAF00002207421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119885188
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC98378408.206051276326146No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG14990341.2503913327474616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC12895991.075695022474336No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA12385431.0331076095906027Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG12201721.017783781596105No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC11745550.9797332094103235No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC9561220.7975313847779093No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT8995710.7503604198376866No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC8797020.7337870630023119No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA8500520.7090550669195264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC8246520.6878681292971739No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC7788400.6496549014879136No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT7715760.6435957709804817Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA7163070.5974941624982062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC6999070.5838144074979471No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC6803770.5675238212080045No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT6199730.5171389479741234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA6157950.5136539469746672No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG6153010.5132418860618545No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC5794160.48330908068476314No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT5719850.4771106502331214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA5705280.47589532077974467No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT5408450.45113579836067824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5086690.4242967863552919No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5075260.423343374162286Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC4880640.40710950880771024No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC4501000.37544254424491535No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC4310250.3595314877430897No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA4276600.35672463557382916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3505490.2924039289991354No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3391680.2829106795077971No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT3364340.2806301642534856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG3321340.27704339922292986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC3210540.2678012232837304No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT3182970.26550152300716245Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT3150460.26278976181778185No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC3121670.26038829751011444No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT3077070.2566680714551659No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC3012330.2512679047556734No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC2930630.24445305119761748No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2624110.2188852554495723No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA2610710.21776751937028285No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC2563960.21386795506380657No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC2537960.211699213417424No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC2521640.21033791096861776No Hit
ACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTAGATCGGAAGAGCACAC2465150.20562590267615044No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2462590.205412365037122No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG2441040.20361481186483188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG2384730.19891781793760877No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC2340800.19525347868662474No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG2304560.19223058648412844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC2275950.1898441365417052No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACA2274170.18969566115206826No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT2253320.18795649717794996No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG2241440.18696554907183363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA2193620.1829767327052947Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA2190780.1827398393869975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2181690.18198161394216605No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG2159050.18009314044700836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG2084080.17383965732280454No Hit
ATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGATCGGAAGAGCAC2070750.17272776016333227No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2043710.17047226885109443No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2014370.16802492731629198No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG2014190.1680099129510478Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAGGAGCACACGTC1925060.160575299760968No Hit
AGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTAGATCGGAAGAGCACAC1889780.15763248417310735No Hit
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTC1888950.1575632512667036No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1874090.15632373200265573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT1814880.15138483996872074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG1804380.15050900199614317No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT1797930.14997098724155983No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACAC1773340.1479198581229234No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1770670.14769714503846798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG1733230.14457415706767712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1716990.1432195276700905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCAGGTCGGACGACCGATTTGCACGTAGATCGGAAGAGCACACGTCT1671300.1394083812922744Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT1646180.1373130432093079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG1640720.13685760746356754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC1632070.13613608380044415No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1622880.13536951704158814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC1592330.1328212456070887No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1529560.12758540279387975Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAGCTTTTTAACTGCAGCAACTTTAATAGATCGGAAGAGCACACGTCTG1513250.12622493447647595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACAC1510450.1259913776837886No Hit
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC1509660.12592548130299466No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1469460.12257227306512629No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACA1462860.1220217463395061No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG1447860.12077054923582387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1447300.1207238378772864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1438740.12000982139678508Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG1437650.1199189010739175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1414320.11797287251199039No Hit
ATGTCTCTTCACCGTGCCAGACTAGAGTCAGATCGGAAGAGCACACGTCTG1407570.11740983381533339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1404750.11717460875984112No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTG1395720.11642138810342441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG1391690.11608523314823513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT1371740.11442114100033776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACACG1368640.11416256026557675No Hit
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC1353340.1128863392198209No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1347350.11238669450975045Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGT1333740.11125144167100944No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTAGATCGGAAGAGCAC1324790.11050489406581236No Hit
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT1312050.10944221065908492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCGCGCGCGCGCGCGGACAAACCCTTGTGTAGATCGGAAGAGCACAC1281980.10693397753190328No Hit
ACTGAGCAGGATTACCATGGCAACAACACAGATCGGAAGAGCACACGTCTG1275960.10643183042762547Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTAGATCGGAAGAGCAC1234860.10300355036353616No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1224350.10212687825955613Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTCATGCGCGTCACTAATTAGATGACGAGGCAGATCGGAAGAGCACACGT1219080.10168729101046245No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGACAGATCGGAAGAGCACACGT1209120.10085649613361745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)