FastQCFastQC Report
Tue 23 May 2023
EGAF00002207422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125880071
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC78909506.2686253171878175No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT47396883.765240964949885Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA39932123.172235261926409Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC38297313.0423648235787857No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT35720602.8376691970566177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT24177131.920647947521415No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC18316321.4550611430780016No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC14745531.171395113051692No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT14163201.1251344146445548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT13872751.1020608655360544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC13808601.0969647451183913No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT11679340.9278148564120209Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC11173620.8876401094498906No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG10237740.8132931542436134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC8879630.7054039554839463No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT8551310.6793219873541381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG8184150.6501545427313907No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA7111810.5649671106397771Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT7087940.5630708613121136No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG6861640.5450934326212764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG6032230.4792045279351646No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA4225830.3357028611780811Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG4157520.33027626748002076No Hit
TTTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT4080820.32418316637269773Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT3977420.3159689987782101No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC3576880.28414982384304505Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3413460.27116762589051924No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC3026040.24039071283968372No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC2748650.21835465917396887Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC2671270.2122075383958117Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2651210.21061395810620412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2597840.20637420835264703Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT2590430.20578555282193953No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2530120.20099448466310443No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2481860.19716067684772756No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2258690.17943189752411245No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2249750.17872169773402813Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC2163330.1718564330965463No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT2093220.1662868461521602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT2057780.16347146801339188No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGAA1863420.14803137503791208Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG1843070.1464147569475076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1818180.14443747811359275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT1803220.14324904535524133Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1777850.14123363498897296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG1731420.13754520364069386Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1699510.13501025114610873No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCC1602230.1272822605891285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1601640.12723539058061065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC1595960.12678416744776067Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT1593700.1266046314829295Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1589590.12627813023715245Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT1565430.12435884310869193No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAAC1540190.12235376003243595Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1537900.12217184084683269No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1515780.12041461273087462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACACGT1431010.11368042523585802No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC1408850.1119200194922038No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAGGAGCACACGTC1398940.11113276223048842No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1374030.10915389458272548No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1358460.10791700300200817No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT1339330.10639730255633555No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA1320020.10486330278603037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC1295000.10287569666210308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)