FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002207426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233934617
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC141860956.064128166204663No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT83358493.5633242770564393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT54653082.3362544928525906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC48072482.0549536710934917No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC45112011.9284024988913888No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC31472261.3453442848092891No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC25020081.0695330311033018No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT24390811.0426336346792147No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT23738851.014764309123177Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT22451790.9597463722096333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC17285150.7388880799971558No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT16847240.7201687469794178No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA16599740.7095888677305078Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG16186310.6919159809512074No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG15556690.6650016230817177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC15127850.6466700052348388No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC14603950.6242748588166411No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA13704000.5858047079881299Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT13598420.5812914811149988No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA13399720.572797654825066No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC13045150.5576408556925972No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA12913340.5520063753540161Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT12884910.5507910785174646No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG12568610.537270206572292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG11707400.5004560740149031No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT10070450.4304813938674155No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTC9489690.40565565377611473No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG9111390.3894844686453566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT9023150.3857124745244523No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC8895200.38024299755516733No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT8632750.3690240508526363No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG7787970.3329122512894276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG7739690.33084842676362003No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG7491650.3202454641418034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT7467470.319211841999425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTCT7082750.3027662212129982Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG6876210.29393725854604924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT6842730.292506089425833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA6297150.2691841883324177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT6103370.260900677217857Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC5793380.247649538759798No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTG5444760.23274708419917176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAAC5396920.23070206834758447Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC5159040.2205334151123089No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTC4865440.20798289976895554No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC4743930.20278871339507654TruSeq Adapter, Index 6 (100% over 50bp)
AGTAACGGCGAGTGAACAGGGAAGAGCCCAGATCGGAAGAGCACACGTCTG4724320.20195044498266795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGTC4633890.19808483496053086No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCTGA4338460.18545609263121585Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT4267700.1824313158406992Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTCCCTCAGTAACGGCGAGTGAACAGATCGGAAGAGCACACGTCTGA4237170.18112625033173266Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCTG4160220.1778368696925261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT4058290.17347966932144976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4000560.17101188576977472No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGAA3920620.16759469163984397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC3895650.16652729937784283No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA3787740.16191447202531808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGT3761330.16078552410223237No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC3656620.15630948710767334No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG3635390.15540196857654462No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGAA3631480.15523482785790527Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG3507140.14991966751120037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGT3441420.14711033553447972No Hit
AGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACGTCT3379700.14447199150521617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCATATGCTTGTCTCAAAGATTAAGCCATGCAGATCGGAAGAGCACACGT3341810.14285230817292852No Hit
AAGGAGTGTGTAACAACTCACCTGCCGAATCAGATCGGAAGAGCACACGTC3174520.13570116474040267No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT3165940.13533439559310712No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACG3161990.1351655449949932No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGAA2957550.12642635100045924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAAAGGTGTTGGTTGATATAGACAGCAGATCGGAAGAGCACACGTCTGA2948480.1260386358296002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG2927900.12515890283993325Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCTGAACT2910470.12441382285888881Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT2840460.12142110630852039No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC2833130.1211077708948052No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACGT2822280.12064396608732772No Hit
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC2781830.1189148504686675No Hit
AAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTGA2714960.11605635945705292Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2693790.11515140574513605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGATCGGAAGAGCACACGTCTG2689450.11496588382214507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2659280.11367620722844965No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT2626720.1122843653361486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGTC2605410.11137342704607073No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2583030.11041674948004808Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCTGA2582090.11037656731239567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC2578230.11021156394309954No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT2538670.1085204931427485No Hit
AAATACCGGCACGAGACCGATAGTCAACAGATCGGAAGAGCACACGTCTGA2532280.10824733989668574Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2520980.10776429894511935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT2515230.10751850377064973No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTC2429720.10386320892388493No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAAGATCGGAAGAGCACACGTCTGAA2395770.10241194871984252Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGATCGGAAGAGCACACGTC2393630.10232047016795295No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGACAGATCGGAAGAGCACACGTCTG2367000.10118211790775711Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGTCCTAAGGCGAGCTCAGGGAGGACAAGATCGGAAGAGCACACGTCT2340220.10003735359953161Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)