FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002207427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129413716
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC85065656.573155661491089No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT81072116.264568587150377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC45276633.4985959293526507No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT28691142.2170092078957073Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT24408001.8860442891540183No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT20913341.6160064517427193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC20882971.6136597143999791No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC17088361.3204442719193692No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG13844791.0698085510503383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT12344130.9538502085822185Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA7702680.5951981164036739No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC7026410.5429416770630402No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG6239900.48216682071010153No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT6167190.47654840542558874No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG6122180.4730704124128543No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5631680.43516871117432404No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT5622160.4344330859025793No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC5484850.42382292770265556No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC4771040.36866571391860814No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG4616970.35676048433691526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG4216790.3258379505925013No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT4186770.32351825829651626No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4137390.31970258855715106No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA4025180.31103194656739475Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG3962640.3061993830700294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA3956740.3057434808532969Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG3919660.30287825132847584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3892650.3007911464345866No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT3779770.2920687324981843No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC3736660.28873755545355023No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGAGATCGGAAGAGCACACGTC3716290.28716353373239045No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACGT3582230.2768045081094805No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3531650.2728961124955256No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTC3462810.26757673815656446No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3334810.25768597820033234No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG3327310.25710644148414685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG3281390.25355813134984856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC3205970.2477303101318874No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG3158040.2440266841576514No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA3103460.23980920229506433Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG3063050.23668665846825693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG3050100.23568599173831004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT2983340.23052734224863772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC2933280.226659127847005Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2814380.2174715391064113No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA2795170.21598715239735483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA2742050.21188248701551848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT2729230.21089186558865214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2712400.20959138519753193No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT2682430.20727555647965476No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2638070.20384778998232306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG2602100.20106833189149748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA2601920.20105442301030907No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG2411390.18633187227233317No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT2363780.18265297319798776No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT2352220.18175971393944054No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT2348230.18145140040642987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2347660.1814073556159998No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2317220.17905520926390833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2301020.17780340995694768Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT2297280.17751441431447654No Hit
AGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACA2215550.17119900953929798No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA2170770.1677387889858599Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2166130.1673802489374465No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG2158410.16678371247758622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGTC2146790.16588581692530954No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2144000.16567022926688854No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC2063600.1594575956693802No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCACA2036360.15735271831619457No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT1974020.15253560913126085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG1941070.14998951115815265Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCCGCCCCCCGACCCGCGCGCGGCAGATCGGAAGAGCACACGTC1901930.14696510221528605No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1858570.1436146072801124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT1849430.1429083452019877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT1830460.14144250366784925No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGT1811110.1399472989400907No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC1747390.1350235549993789No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCTGA1709600.13210346266542566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC1678210.12967790832928403No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTC1648710.12739839724562116No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC1634300.12628491403492348No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1598860.1235464098720417No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAGATCGGAAGAGCAC1476010.11405359846092357No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTCT1455610.11247725859289906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1448020.11189076743611938Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG1424890.11010347620340336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA1400370.10820877749928763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1386680.10715092981334375Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA1376970.10640062294478894Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAAAGGTGTTGGTTGATATAGACAGCAGATCGGAAGAGCACACGTCTGA1371220.10595631146238008Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1368890.10577626872255179No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA1339380.10349598492326734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTC1318060.1018485552180574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)