FastQCFastQC Report
Tue 28 Mar 2023
EGAF00002207428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129195382
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT83358056.452092072455036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC41003043.173723345622369No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG32683722.529790112776632Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT21430051.658731888729583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC15612491.2084402521446163No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG14145131.094863437146693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC12972841.004125673779888No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT8621240.6673024891864943Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTG7921410.6131341443767704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT7538100.5834651272597344No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG7499190.5804534097046905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT7123480.551372648907838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT6744240.5220186585306896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAA6393440.49486598522538516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA5260990.407211923410699Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC4982530.38565852144777124No Hit
AGTCAACAAGTACCGTAAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGA4795400.37117425760620454Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC4580730.35455833862544717No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT4430310.3429155076146607No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG4333240.3354020811672665No Hit
GTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTGA4329120.3350831843200092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC4152210.3213899704247943TruSeq Adapter, Index 3 (100% over 50bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG4060890.3143216063249072No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA3973310.3075427262562682Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT3806080.29459876514781314No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC3666610.283803487650975Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT3296470.25515385681509883No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG3163460.2448585971904166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT3150840.2438817820903227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT3027700.23435048166040484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA3002820.23242471623327837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG2978240.23052217145036963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT2827810.2188785664181093No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2818610.21816646666209788No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA2807330.21729337043950997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTGA2798810.21663390414372552Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA2721070.21061666120542913Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2714540.21011122518295586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAAC2612260.20219453354764647Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACACGTCTGAAC2598240.201109355441203Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2439230.18880163998431462No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2400760.18582397937412345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAA2317580.17938566875401166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT2317310.17936477017421568Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT2279640.17644903128193856Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG2239970.17337848809487633No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2189530.16947432378039642Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2187200.16929397677697178Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2113090.16355770363371036No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC2100910.1626149454784692Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2079370.1609477032236338No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTAGATCGGAAGAGCACACGTC2070310.16024643976825736No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA2027090.1569011189579516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCT1971630.15260839586356115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAAC1969840.15246984601972846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1957020.15147755049015604No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGTC1881130.1456035015245359No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC1852380.1433781897870003Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1782020.1379321746964609Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGAAC1773150.13724561764908905Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC1763900.136529647785708No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1758150.13608458543820087No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACGT1715070.1327501009285301No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1671160.1293513726365235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1655920.12817176391026114Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC1624280.12572275996676102Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT1545030.11958863978590195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC1524050.11796474273360637No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG1506290.1165900805959148Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT1488790.11523554301654529Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1486310.11504358569101178Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1439730.11143819366546709Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT1434850.11106047118619147No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1426830.11043970596410325No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1407890.1089737092924885No Hit
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGAA1406020.10882896727686443Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC1400730.10841950991715787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1385960.10727628020017No Hit
CTTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAAC1375270.10644885124454372Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1373760.10633197400198097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGA1369760.10602236541241078Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGT1363150.10551073721814608No Hit
AAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTGA1347010.10426146655923042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGAA1332440.10313371727172105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACT1332020.10310120836981619Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACG1328120.10279933999498528No Hit
CAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTG1299500.10058409053661066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)