FastQCFastQC Report
Mon 3 Apr 2023
EGAF00002207430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126568881
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC79423106.2750890560532016No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG36482972.8824597098239337No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT19804421.5647147895697995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT18101271.4301516973986679Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT13557751.0711756233350915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC12385350.9785462194297191No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC11067750.8744448013252167No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG10384840.8204892006590466Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA9555090.7549320120796517Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT9158730.7236162576170678No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC8849510.699185291841207No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT8837140.6982079584001379Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT8804440.695624384954466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG8710060.6881675757250315No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC8536110.6744240711111288No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC7886140.6230710058975713No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC7365450.5819321417560767No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC7270200.5744065952514821No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA7208080.5694985957883281Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC7061460.5579143897147989No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA6533630.5162114058668181Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC6100320.4819762924189872No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC6041090.47729662712274434No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG5415850.4278974387077025No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT5341880.42205319015185094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT5282950.417397227364284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC4857720.38380050148345707Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT4255720.33623746740717414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA4190530.33108691227190357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC4164760.32905086677664475No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3943990.31160819064205836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA3922850.3099379538640307No Hit
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG3894080.3076648832820131Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA3862960.30520614304870086No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC3801690.30036530069346196No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA3581650.28298030066332025No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT3567070.28182835874167206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG3329240.2630377999470502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG3306540.261244310123908Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT3206750.2533600656546849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT3187980.2518770786951968No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA3125760.24696117839581752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC3121090.2465922093440962No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT2982370.23563216933236536No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2971390.23476465751482786No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG2893430.22860516559358693Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2868460.2266323267881305No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2826570.22332266649335392No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG2807540.22181913735968006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG2799480.2211823299599212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2780790.21970566366941333No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA2750560.21731724087850632Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT2685050.2121414030673148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC2614440.20656262260863317No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTGA2568040.20289663460009577Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG2555500.20190586973744357No Hit
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA2508210.19816956428650104Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT2504090.19784404983401882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2475940.19561996443659796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT2460170.19437400256386875Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC2416300.19090790571183133No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC2344070.1852011317062999No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC2308050.18235525049794823No Hit
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTGA2292610.1811353613847625Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC2260720.17861578471251555No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC2212470.17480363123381015No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG2181990.17239545635233988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC2110970.16678428246513455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGATCGGAAGAGCAC2103420.16618776933012466No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2072630.163755101856356No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC2054510.16232347033233233No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA2038910.1610909398811861Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGATCGGAAGAGCACACGTC2027560.160194194969615No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG2014650.15917419701292926No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT2013650.15909518865067632No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC1982270.15661590624317837No Hit
ACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGTCTGAA1978610.15632673563733254Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT1949740.15404576421908953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT1917030.15146140068979513No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT1915460.15133735756105798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1853270.14642382751254632Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA1819220.14373359277783299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC1795840.14188637726835873No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1761580.13917955077757224No Hit
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGA1723890.13620172560425814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1701710.13444932012948743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGACGTCGCCGCCGACCCCGTGCGCTAGATCGGAAGAGCACACGTCTG1687270.13330843937855466Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1675840.13240537379800332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG1645970.13004539401750734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1632980.12901907539184138Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1592480.12581923672059644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1580850.12490036946759449No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA1509470.11926075256997809Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAAC1485530.11736929237764217Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT1481120.11702086550010662Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1458260.11521473433900391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT1387930.10965807622175312Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCT1377110.10880320574217607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCAGGTCGGACGACCGATTTGCACGTAGATCGGAAGAGCACACGTCT1370060.10824619678829268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA1345380.10629627040788961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC1334390.10542796850672954No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTAGATCGGAAGAGCACACGTCTG1326930.10483856612432246Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAAGGATCAAAAAGCGACGTCGCTAGATCGGAAGAGCACACGTCTGAA1315480.10393392037652606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACACCTGCGTTACCGTTTGACAGGTGTAGATCGGAAGAGCACACGTCT1299150.10264371382093518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)