FastQCFastQC Report
Tue 23 May 2023
EGAF00002207431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113031832
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC38158163.375877336925761No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC32080732.8382031355556547No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC14015611.23997017052683No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT12736961.126847169919355Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG12342151.0919180713624104No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA12274621.08594364815745Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT10954020.969109303651736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA10188610.9013929810497985Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG8162550.7221461295964839No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC7799070.6899888166016809No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT7062530.6248266417552181Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT6951660.6150179004441864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC6676890.5907088190873523No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC6305180.5578233926174001No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT6033800.5338142267746311No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT5769840.5104615131779869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC4972640.4399327085134744No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4717390.41735057430547534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG4666650.4128615733663416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA4478150.3961848552538722No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA4266770.37748392859809615Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3979310.35205215465321305No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3528400.31215985245643013No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC3482010.30805569885835343No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3385790.29954305261547914No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG3335740.2951150964269959No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC3158680.27945048258617977Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG3115510.2756312044911384No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT3023470.26748836557829125No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2926140.2588775169104576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2884190.2551661730122184Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA2857270.2527845430303209Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGC2804950.24815575845926305No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2696690.2385779255528655No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2557380.2262530788671991Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC2552500.22582134207999033No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC2505160.22163314136145296No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT2486920.2200194366486071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2466180.21818455530296987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2427490.2147616257338906No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2417090.21384153094147848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2353850.20824664683838798Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA2309350.20430970277470153Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC2243230.19846002319063535No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC2229600.1972541681886568No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC2211140.19562099993212534No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1952990.17278230083008828No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA1937410.17140392805453247No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1919690.16983622808130722No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1912020.16915765817190329No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG1868930.16534545772911122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT1834640.16231179903374476No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1821210.1611236381623895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTC1803930.15959486527653555Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACG1786080.15801566411840517No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGT1781000.15756623320057309No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1776210.15714245877214483No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCTG1758210.15554998701604694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1720870.15224649282867503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT1716640.15187226196599202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1698600.1502762513837695No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA1681640.1487757891069128No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTGA1674530.14814676276325417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC1602810.1418016475217353Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1596210.14121774121116606No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT1581420.1399092602515723Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC1531100.1354574169867476No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC1516780.13419051723411862No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1506570.13328723186579866No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1503590.13302358931951136No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC1486910.1315478988255273No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCA1437950.1272163756489411No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1418290.12547704260866974No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGT1410300.1247701620902685No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC1394810.1233997516734932No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1383340.12238499328224638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC1356030.11996885974563341No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1346920.11916289209574166No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1345610.11904699554015899No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC1316430.1164654218822181No Hit
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC1316080.11643445715362731Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1293710.11445536864341012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT1288290.11397585770351842No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC1278410.11310176765072692No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC1272760.11260190846061843No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAAC1270770.11242585186091648Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG1266640.11206046806354515No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC1233180.10910024001026543No Hit
GTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGATCGGAAGAGCACACGTC1224270.10831196649099697No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1203730.10649477927598308No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1161520.10276043300793355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT1152540.10196596654294693No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1146340.10141744849362433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1144890.10128916604660534Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA1143850.10119715656736414Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1137980.10067783383357No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAA1137120.10060174907188978Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)