FastQCFastQC Report
Tue 23 May 2023
EGAF00002207432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76618769
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC34147224.456769593883713No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG11497731.500641442046661No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT10301941.3445713282081053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG8585441.1205400598383406Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA8171621.066529795068882Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT7745671.0109363673018552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT7051110.9202849500231465Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC6123310.7991919055760345No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5008460.653685782918282No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC4946710.645626399975181No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT4841700.6319208809006055No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC3660640.47777327249932716No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA3584950.4678944920140912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC3544100.46256290022096286No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA3512650.4584581618637073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC3398500.44355972359722984No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3058040.3991241362804981Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT3034530.39605569752758624Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC2719080.3548843234482141No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG2695710.3518341569805174No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA2289970.2988784641006174Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2274070.2968032545654708No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA2231220.29121063012641196No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2076420.27100670333139915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2074010.270692158993053No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2054550.2681523113481502No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC2004060.2615625422016373Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT1967350.25677128798558485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1916140.25008754708653697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC1801280.23509644223075418No Hit
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT1798980.23479625468793425Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA1779940.23231122389867684No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1726030.22527508892762294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG1725160.2251615397266432No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1701300.22204742026069357No Hit
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA1684470.21985083054518925Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA1674600.21856263443752275Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1673450.2184125406661128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1641890.21429344551333107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT1635490.2134581410463538Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1624350.2120041892085215No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT1619910.21142469673455597No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1617430.21110101625360228No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1598490.20862903709664143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC1571880.20515599774253745Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1562370.20391478751113842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1541690.2012157099522181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG1537020.20060619872397062Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1520980.19851271690360883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1517820.1981002853230388No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1512080.19735112162921853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT1467880.19158230015415675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1427970.18637339370461564Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1425700.18607712165148463Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA1423790.1858278354746211Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC1406770.1836064476577534No Hit
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC1402380.1830334809999362No Hit
ACAAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAAC1373540.17926939024561986Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1359460.17743172041826985No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1352580.17653376811626928No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT1316200.1717855842867953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTGA1309000.1708458667614459Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT1259760.16441924301863947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1251920.1633959950465923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1229630.16048678620769802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1227900.1602609929689682Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1220940.15935259936113041Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1216500.15877310688716495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1189170.15520609578052605No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG1178010.15374953361623445No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC1176080.1534976371129116No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG1172730.15306040743097818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1167400.15236475542957367Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1119220.14607647898911036Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1117370.14583502379162475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT1085920.14173028543436922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC1085550.1416819943948721No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA1078380.14074619235921162Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1045140.1364078297838484No Hit
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT1040200.13576307914840033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGTCTGAA1007480.131492585060979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA978350.12769064457300272No Hit
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG977830.1276227760850608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT963850.12579815789000734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAACACAAAGCACCCAACTTACACTTAGATCGGAAGAGCACACGTCTG956530.12484277840590209Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC952940.12437422480645702No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA944530.12327658253031969No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG939140.12257309954953727Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA935980.12216066796896724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTC927420.12104344824438514No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT910160.1187907365100058Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA897180.1170966346379175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG895710.11690477564315865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC894430.1167377147497632No Hit
ACGGAATCGAGAAAGAGCTATCAATCTGTCAGATCGGAAGAGCACACGTCT876960.11445759458756116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG864650.11285093865185958No Hit
AAGGGCATCACAGACCTGTTATTGCTCAGATCGGAAGAGCACACGTCTGAA845120.11030195486434924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT835440.1090385568580461Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG831230.1084890831383626Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTGA808750.10555507619810493Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAG804900.10505258835468892Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGAGCAGGATTACCATGGCAACAACACAGATCGGAAGAGCACACGTCTG793040.10350466476432166Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA786860.10269807388839673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG771870.10074163420714839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC770880.10061242304741284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)