FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002207433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214451799
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT70211713.2740089067753635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA64142852.991014778104053Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC34235121.5964016230985312No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC28789881.3424872225016866No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT25118241.171276721255204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC23805981.1100853483630604No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC22169761.033787550553493No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG19994930.9323740856097924Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG19644940.9160538681235311No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG17572980.8194372852987818No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG15701370.7321631281815453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC14736460.6871688681893501Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT14145070.659592041939457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC11207040.5225901602252355No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC10915500.5089954969321567No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT9763690.4552859917952938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT9605660.4479169699108003No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG9407510.43867713135854836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA7392870.34473341023359755No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC7304750.34062432836014583No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA7054760.3289671633857453Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG7027940.32771653270206424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC6991690.3260261761665147No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT6936580.32345636792722826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT6521040.30407951951944223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG6504950.3033292343702838No Hit
AACCTATTCTCAAACTTTAAATGGGTAGATCGGAAGAGCACACGTCTGAAC6261420.2919733025881494Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG6210310.2895900164493374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA5898530.275051551327858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTCTGAA5882440.27430126617869965Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA5821510.27146006828322294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA5756760.2684407417817931Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT5728150.26710664245814975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG5247910.24471279907518984Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT4917050.22928462353444748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGAA4650380.21684966140106848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG4277400.19945740814233037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT4200230.19585893051892747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAAC4112850.19178435523406356Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT4008670.18692638712720708No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG3741430.1744648455945105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAAC3572570.1665908151229825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG3419990.1594759296003854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA3411390.15907490708436536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT3400800.1585810898233593No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC3388140.15799074737535776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3296580.1537212564954981No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC3103210.14470431185331303No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG3042580.14187710311537186Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT3016770.14067356926206062Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT3000130.13989763732408697No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTGA2902200.1353311099992218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2896060.13504479857499352No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT2863640.1335330369506483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT2779630.1296156065354341No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA2755560.12849320979582923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA2746060.12805021980720244Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC2721740.12691616543631792No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2706870.12622276952780426No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC2642480.12322023001541713No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT2613740.12188006872350834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGACCTGCTTCTGGGTCGGGGTTTCGTAGATCGGAAGAGCACACGTCT2603090.1213834536309952Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGCGAGTGAACAGGGAAGAGCCCAGATCGGAAGAGCACACGTCTGAAC2575760.12010904137950365Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT2575640.12010344571648941Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCTGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2569040.11979568425070661Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGAATCCGACTGTTTAATTAAAACAGATCGGAAGAGCACACGTCTGAA2549070.11886447266408803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2545580.11870173213142408No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2524590.11772295740918451Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC2483710.11581670154233586No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTC2369890.11050921517333598No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2332580.10876943028116076No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG2318090.10809375397219213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2297820.10714855322803797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2244360.10465568535519723No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2242290.10455916016820171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG2186000.10193432790927531No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC2165340.1009709412603249Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTGAAC2151910.10034469330798199Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)