FastQCFastQC Report
Thu 23 Feb 2023
EGAF00002207434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206927165
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC137767046.657755157472921No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG43732892.1134436360735913No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT16068970.7765519814665223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA15254460.7371898223222649Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA14651830.7080670147875462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC14428960.6972965584291458No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG14214120.686914161318549Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC13691350.6616506827414371No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA13626180.6585012654090148No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG12056050.5826228760250013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC11446240.5531530864978507No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA11131280.537932271966322Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC11040230.5335321730232955No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC10930890.5282481881970402No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT10456210.505308715750298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT10300950.4978055926103274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC9824450.4747781665109073No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT9719780.46971986495828133No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA8516610.4115752516108748No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC8448560.40828665487201743No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT8094770.3911893346627544No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC8091930.39105208830363086No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT7660670.37021093871362903No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC7122340.3441955047323052No Hit
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC6925920.3347032759086996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC6918630.3343509780361607No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG6651430.3214382219946811Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC6564410.317232877568298No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA6376330.30814368910916073No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC6084640.29404742485115476No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6059460.29283057156850334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT5999240.28992036884089145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG5914910.28584502184621335No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5563150.268845803788014No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT5432370.2625257056027419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG5390690.260511470304056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC5205160.25154551361103317No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC5064230.24473490467044287No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG4994580.24136898603912155Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG4892840.23645228020207013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG4787900.23138093058009085No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT4774410.23072901037425414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA4653720.22489652337333282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC4621380.22333365462190524No Hit
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG4548250.21979956087447486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGACCCCACACCCCCGCCGCAGATCGGAAGAGCACACGTCTGAACTC4546720.2197256218148062Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT4523900.2186228183235391No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC4437990.21447111596005292No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAA4436310.21438992797296577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC4417800.21349541032952343No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT4395040.21239550640922375No Hit
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT4359480.21067702734921248No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC4167190.2013843856605294No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCAGATCGGAAGAGCACACGTC3870220.18703295915739243No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG3801990.18373566370563285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA3728000.18016000944100308Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3722500.17989421543565823No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT3695400.1785845758820501Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT3538750.1710142793480015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT3512660.16975344923901123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA3448020.16662964478346765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG3436820.16608839153622001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTAGATCGGAAGAGCAC3432100.16586029195345137No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT3421550.1653504507250172Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACGT3402120.1644114729934081No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGATCGGAAGAGCACACGTCTGAA3392710.16395672361335448Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3327020.16078217666588146No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC3287730.1588834409440636No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3284470.15872589758816827No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT3271370.15809282459361967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACACGTCTGAACTCC3195550.15442873341448426Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG3057950.1477790506625846Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGCAGGATCAACCAGGTAGGTAGGCAGATCGGAAGAGCACACGTCTGA3054520.147613291855615Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG3051270.1474562317615476Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG3032090.146529335575636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG2928090.14150341256547927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGAGATCGGAAGAGCACACGTC2910430.1406499721774084No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2906620.14046584942097864No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACACG2866190.1385120218507802No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG2828040.1366683779773429No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2789980.13482908346035669No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2760690.13341360956643852No Hit
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC2736030.1322218858988379No Hit
ATACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTG2725160.13169658029191092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2722500.13156803264568961No Hit
ACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTAGATCGGAAGAGCACAC2715090.13120993563121594No Hit
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2707140.1308257424780357Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTC2662940.12868972519871907No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTCT2660350.12856456038529304Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT2644980.12782178695581126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG2637000.12743614401714728Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC2587310.12503481599431374No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT2562220.12382231206811345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGATCGGAAGAGCACACGT2555030.12347484681385357No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG2498520.12074393422439245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC2496510.1206467985969846No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG2449930.11839576500262784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2446070.11820922593705858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT2436600.11775157698603758Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA2430700.11746645250757676Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT2415730.11674300955121092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTAGATCGGAAGAGCAC2389060.11545415025620245No Hit
CCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACACG2351390.11363370295050434No Hit
CCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACAC2346440.113394488345694No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC2309660.11161705134267895No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT2298650.1110849800701614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGCACACGTCTGAACTC2289200.1106282976427962Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC2284240.11038859977615795No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA2282430.1103011293853081Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACT2270440.10972169845365638Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA2258970.10916739713705545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCAGATCGGAAGAGCA2232790.10790221767161406No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA2221040.10733438502383194Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAAC2212380.10691588028087082Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG2207690.10668923048358586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTCTCTTCACCGTGCCAGACTAGAGTCAGATCGGAAGAGCACACGTCTG2207320.10667134979595358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAGCACACGTC2206130.10661384163843352No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC2188990.10578553086541345No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA2181650.10543081668373507Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2155310.10415790502904729Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT2104380.10169665253955419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)