FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002207435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146563737
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC57547503.926448736770406No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG30316232.0684673180788233No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC25788131.7595164075271907No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC18049511.2315126762904522No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC16866391.1507887520635476No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC15968771.089544407563789No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG12281580.8379685351500009No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG12124660.8272619304187092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC11551730.7881710876408672No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT9351640.6380596040615422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC9053710.6177319291469758No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA8772440.5985409610564174Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA8576490.5851713510825669No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC8410170.5738233871588577No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT8248320.5627804100000534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC7975540.5441687120737103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7557310.5156330040902274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA7284240.4970015195505011Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG6992710.4771105147243892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA6394780.436313929413522No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA6392620.43616655325866865Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT6291790.42928695247447196No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC5843360.39869070751109464No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG5767850.3935386827643457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT5707610.3894285255567685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT5690790.38828090198054926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC5412170.36927074259849146No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC5293960.3612053096053357No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC5174300.35304094354526455No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5144770.35102612046525533No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT5139570.35067132601838613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACA5099080.3479087054118987No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACA4968000.3389651561627417No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC4934760.33669720089083155No Hit
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT4914960.33534625280467567No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT4795760.32721327240721215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC4705550.3210582710510445No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG4545210.3101183207412349No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA4445070.30328579845094966No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC4128230.28166789988440316No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT4049480.2762948109053742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC3987980.27209868427413253No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC3987800.27208640292789477Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG3932000.26827918559418285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC3857880.2632220001322701No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT3749210.25580747848971674No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTCCAGATCGGAAGAGC3517900.2400252662771556No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT3411910.23279360023414247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA3402510.23215224104172508Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCTGTTCACCTTGGAGACCTGCTGCGGATAGATCGGAAGAGCACACG3320230.22653830121703294No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT3303970.22542888627355345No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG3185770.21736413557741094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTAGATCGGAAGAGCAC3100560.21155028272784832No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC3042500.20758886626915085No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG3040800.20747287577690515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG2968000.2025057535207362Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC2933460.20014909963710875No Hit
AGTCCGCCCCGCCCCCCGACCCGCGCGCGGCAGATCGGAAGAGCACACGTC2922900.19942859399115892No Hit
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT2910410.19857640502165963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG2902310.19802374444095946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG2825910.1928109952600349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA2722770.18577378386578666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT2658260.18137228583356876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT2636990.17992104008647106No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT2598700.17730852482288986Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC2598670.17730647793185023No Hit
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT2530930.1726845979643655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT2512400.1714203015988873Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2474800.1688548648292176No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGT2443190.16669812397046072No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC2417860.16496986563599972No Hit
AACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCAGATCGGAAGAGCACAC2415190.16478769233347262No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAGATCGGAAGAGC2410220.16444859071790724No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA2387860.16292297459636962Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC2371880.16183266396926002No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG2348110.16021084396885976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG2321390.15838774634956257No Hit
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG2304420.15722988831814516No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2303530.15716916388396948No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG2239260.15278404098006862Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT2221380.15156409392044912Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA2210600.15082857774020866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC2184360.14903823037754557No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT2152400.14685760912332632No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2140220.1460265713612365Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT2136180.14575092336789966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA2132570.14550461414613083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGT2120880.14470700893768831No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG2120140.1446565189587108Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2079940.14191368496560647No Hit
ATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGATCGGAAGAGCACACG2056500.14031438076664215No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG2047950.13973101682034758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTATCCAAGTAGGAGAGGAGCGAGCGACCAGATCGGAAGAGCACACGTC2020240.1378403717967426No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT2012830.13733478970995397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGACAGATCGGAAGAGCACACGT1949300.13300015678503066No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC1936660.1321377333603332No Hit
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCAGATCGGAAGAG1936250.13210975918279158No Hit
ACTGAGCAGGATTACCATGGCAACAACACAGATCGGAAGAGCACACGTCTG1923680.13125211183718657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACAC1909520.13028597926648117No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG1899650.1296125521144429Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCCGCTTCGCGCCCCAGCCCGACCGACCCAGATCGGAAGAGCACACGT1896880.1294235558417837No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1874880.12792250241272166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGT1860780.1269604636240955No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA1759100.12002286759377594Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGATCCAACTACGAGCTTTTTAACTGCAGCAGATCGGAAGAGCACACGTC1750140.11941152946993977No Hit
ACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGTCTG1724470.11766007303702962Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC1712320.11683108216597943No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1681690.11474120641451711Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT1680010.11462658051629784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACAC1666990.11373822980509837No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA1662060.11340185737758583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCT1657050.11306002657396762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAA1641130.11197380972893724Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC1622830.1107252061947629No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1612360.11001084122193198No Hit
AATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCAC1611760.1099699034011394No Hit
ACTGAGCAGGATTACCATGGCAACAACACATCAGATCGGAAGAGCACACGT1597880.10902287514680388No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1572020.10725845507064274No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1570600.10716156889476693No Hit
ACCTTGGAGACCTGCTGCGGATATGGGTAGATCGGAAGAGCACACGTCTGA1555130.106106055415331Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGACGTCGCCGCCGACCCCGTGCGCTAGATCGGAAGAGCACACGTCTG1549930.1057512609684618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGT1549850.10574580259235611No Hit
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC1536680.1048472174259585No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT1528520.10429046306317913No Hit
ACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACT1526110.10412602948299551Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACAC1516680.10348262339953844No Hit
AGGAAGAGCCGACATCGAAGGATCAAAAAGCAGATCGGAAGAGCACACGTC1494090.10194131444669702No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC1490160.10167317172050545No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG1486390.1014159457465253No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA1476470.10073910710942094Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT1469420.10025808771510786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)