FastQCFastQC Report
Tue 23 May 2023
EGAF00002207436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88774680
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC58485416.5880733109936305No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC14261441.6064760807924061No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG11166581.2578564068042826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA11059991.2458496048648107Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG10340281.1647780650969397No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC8524950.9602907045116917No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT7472480.8417355038621374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC5584280.6290397216864088No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5526800.6225649025149964No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC5224870.588554078708028No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC5203340.5861288376370379No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT5037870.5674895139019369Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT4984660.5614956877343855No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC4749920.535053463442504No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC4291520.48341711848468505No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA4024840.45337702146603065Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3662910.41260751376405974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA3483690.3924193249696873No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3409800.38409600575299174No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT3265460.3678368651962474Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC3194340.35982557188603775No Hit
ACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTAGATCGGAAGAGCACAC3157360.3556599697120846No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2947830.33205751910342No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT2742480.308925923472774No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT2741690.30883693413482316No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT2525730.2845101779020775No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2504840.28215702945930077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC2502520.2818956936820273No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2442430.27512687176118233No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2375300.2675650309299904No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA2355550.26534029748121873Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2347350.2644166106822351No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA2305580.2597114402439975No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC2167910.2442036400469143No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2005580.22591802076898504No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC1967480.22162625649565845No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT1944620.21905119793166247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTAGATCGGAAGAGCAC1875410.2112550560587771No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT1872850.21096668554592368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG1864150.20998667638114835No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1772940.1997123504134287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC1759140.1981578531175781No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1688300.19017810033221183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1687440.19008122586305012No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1683380.18962388825282164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG1653540.18626256946237374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG1631130.1837382010275903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG1597410.1799398206785989No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1593940.17954894346000458Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC1541520.17364410663040408No Hit
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG1492480.16812000899355536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1488830.16770885572327607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1481830.16692034260219243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG1473570.16598989711931375No Hit
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG1447590.16306338699277767No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1432700.16138610693950123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT1415840.1594869167650055No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1402770.1580146501232108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1364330.1536845866411459No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC1362460.15347394099308498No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT1358890.1530717993013323No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1353480.15246239130346625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1345670.15158263594980012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1247850.14056372830631436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA1233430.1389393912768821Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1232350.13881773496677205Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1214400.13679576203485047No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1193860.13448203924812796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG1192890.13437277385849208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT1182840.13324069430607918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC1172170.13203877502008457No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1171080.13191599226265868Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1165110.13124350321510592No Hit
AGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTAGATCGGAAGAGCACAC1154700.13007087155932298No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1142590.12870674385984832No Hit
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC1104760.12444539366404926No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1084740.12219024613775009No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG1075950.12120009894713221Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT1067360.12023248070283103Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT1065730.12004886979035012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTC1045290.11774641147678595No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1039920.11714150926818323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTCCTCTAGATAGTCAAGTTCGACCGTCTTAGATCGGAAGAGCACACGT1039730.11712010676918239No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT1031370.11617839681314537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1024720.11542930934811593No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG1012000.1139964683623754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG1000800.1127348473686416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGT994520.112027438454298No Hit
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT988000.11129299480437439No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACACG980830.1104853320789216No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTAGATCGGAAGAGCACACGTCTG980730.11047406760576327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCAGGTCGGACGACCGATTTGCACGTAGATCGGAAGAGCACACGTCT976670.11001672999553476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT968490.10909529609118276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA964260.1086188088765851No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG961090.10826172507746579Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGACATTCAGAGCACTGGGCAGAAATCAGATCGGAAGAGCACACGTC933130.10511217838239462No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT924500.10414005434883009No Hit
ACTGGCAGGATCAACCAGGTAGGTAGGCAGATCGGAAGAGCACACGTCTGA896740.10101303660007561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC895740.10090039186849223No Hit
ACGCGCGCGCGCGCGCGCGGACAAACCCTTGTGTAGATCGGAAGAGCACAC895220.10084181660806887No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC892500.10053542293816209No Hit
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT888760.10011413164204028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)