FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002207438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137035804
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG73105635.3347831636759695No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA50316673.671790038171338No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA30947122.258323671381532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT26200791.9119667441072554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC20621351.5048147562953693No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA16610381.212119717267467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC13849701.0106628775644648No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG11350890.8283156422390167Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC11116260.8111938395311636No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA11062630.8072802637769031Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG11013610.8037030964549965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA10443490.7620993707600678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT10333770.7540927041227853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT10313510.7526142583875379Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGAA8349980.6093283475025256Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC7989140.5829965430056513No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT6597310.48142965615030076Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAAC6507720.4748919486764204Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT6061500.4423296556861884Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT5195000.37909800565697416Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGGCTCGTGCGTCGATGAAGAACGCAGATCGGAAGAGCACACGTCTGA4985590.3638165978870748Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG4969680.3626555874404911No Hit
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA4961330.362046257633516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT4873700.3556515784736083No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG4573330.3337324893573069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG4503180.3286133892424202Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG4462820.3256681735526578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG4196820.3062571880849475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA4042080.2949652486440697Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC4025840.2937801568997253No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTGCAGATCGGAAGAGCACACG3879790.2831223582998791No Hit
AGGCGGGGGACCGGCTATCCGAGGCCAACAGATCGGAAGAGCACACGTCTG3796680.27705751994566324Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC3739590.2728914554330633No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC3708480.27062124581689617No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTCT3656310.2668142115618193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCCGCCCCCCGACCCGCGCGCGGCAGATCGGAAGAGCACACGTC3569740.26049688444926405No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC3554060.25935265793748324No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG3371520.24603205159434097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACGT3359510.2451556383031109No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC3336790.24349767743910197No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3208710.23415121496276986No Hit
AGCCCTGAAAATGGATGGCGCTGGAGCGTAGATCGGAAGAGCACACGTCTG3190160.23279755413410064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA3098500.2261087912469941No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAGATCGGAAGAGCAC3067890.22387506844561586No Hit
GGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACAGATCGGAAGAGCACACG3032540.22129545064003855No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2989130.21812766538006376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT2925940.21351646172703886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA2916700.21284218538973948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC2844340.20756181355348563No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2774580.20247117315413424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2759630.20138021739194525No Hit
CTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTC2735140.1995930932035835No Hit
AAATGGGTAAGAAGCCCGGCTCGCTGGCGTAGATCGGAAGAGCACACGTCT2734340.1995347142999212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2683940.19585684336919715No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTCT2644470.19297657420975908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2636430.1923898662279531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACAGATCGGAAGAGCAC2587920.1888499154571312No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG2581930.1884128034159598Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACG2484730.18131976662099197No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2384280.1739895655298961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCGGACCAGATCGGAAGAGCACACGTCTGAA2371170.1730328812461304Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT2325820.1697235271447745No Hit
AGGCGGGGGACCGGCTATCCGAGGCCAACCAGATCGGAAGAGCACACGTCT2319730.16927911774064536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCAGATCGGAAGAGCACACGTC2303670.16810716124962496No Hit
AAAGGATTTAAAGTGGACTCATTCCAATTAGATCGGAAGAGCACACGTCTG2287330.16691477214232275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTAGATCGGAAGAGCACACGT2267020.16543267772559644No Hit
CGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACAGATCGGAAGAGCACACG2150640.1569400067153253No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC2117910.15455157981924197No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA2099790.1532292976512912Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2091630.15263383283393586No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG2020110.14741475884652744No Hit
ACCGGATAAAACTGCGTGGCGGGGGTGCGTAGATCGGAAGAGCACACGTCT1979490.14445057001307482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGACCAAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTC1961570.1431428825710396No Hit
AGACAGCAGGACGGTGGCCATGGAAGTAGATCGGAAGAGCACACGTCTGAA1938850.14148492170703067Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGGCGAGGCGCCGCGCGGAACCGCGGCCCAGATCGGAAGAGCACACGTC1933250.14107626938139467No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1892700.1381171887020125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGCCGGGCGTGGAATGCGAGTGCCTAGATCGGAAGAGCACACGTCTGAA1892150.13807705320574468Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCAGATCGGAAGAGCACACGTC1863020.13595133137614168No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG1842280.1344378582986969No Hit
AGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAA1806320.13181372657907708Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCAGATCGGAAGAGCACACGTC1780350.1299186014189401No Hit
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC1730050.12624802785117384No Hit
GGAGGTGGAGCACGAGCGCACGTGTTAGATCGGAAGAGCACACGTCTGAAC1719880.12550588603836701Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC1702160.12421279332224737No Hit
AATGGGGCGAAGCTACCATCTGTGGGATTAGATCGGAAGAGCACACGTCTG1695500.12372678894925883Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTGAAC1690710.1233772452635809Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC1667550.1216871760025577No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAGATCGGAAGAGCACACGTCTG1657410.12094722339863823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGT1650770.12046267849824123No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1638800.11958918415219427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1631500.1190564766562759Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA1629650.11892147544155687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC1623750.1184909310270475No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1617350.1180238997977492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAGATCGGAAGAGCACACGTC1614000.11777943813866339No Hit
AACCCCCGGGACGGGGACCGGCGGGCCACGGGCAGATCGGAAGAGCACACG1605150.11713362151689934No Hit
ATACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTG1601660.11687894354967261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG1576630.1150524136013388Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1566680.11432632598703914No Hit
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG1556180.1135601028764716No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1554850.11346304794913305No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACGT1547860.11295296227838383No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1546350.11284277209772126Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCTACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCT1543580.11264063514379061Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCAGATCGGAAGAGCACACGTC1533950.11193789909095583No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1528670.11155259832678474No Hit
AGGTGGGGAGTTTGACTGGGGCGGTAGATCGGAAGAGCACACGTCTGAACT1520110.11092794405759825Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1518120.11078272653473832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGGGGAGTTTGACTGGGGCGGTACAGATCGGAAGAGCACACGTCTGAA1514160.11049375096161Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTAGATCGGAAGAGCAC1501680.10958304006447833No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT1489760.10871319439991027No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1489410.10868765362955801No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATAGATCGGAAGAGCACACGTCTG1478510.10789224106715935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCAGATCGGAAGAGCACACGTC1460280.10656193179995499No Hit
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT1459620.10651376920443362Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGTC1454000.10610365740620605No Hit
AAGGCGCCCGATGCCGACGCTCATCAGACCCCAGATCGGAAGAGCACACGT1447220.10560889619766817No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1403470.10241629990363688No Hit
AGCCGACGTCGCCGCCGACCCCGTGCGCTAGATCGGAAGAGCACACGTCTG1382140.10085977238474114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGCGGAGGAGCGAGGAGGAAGGACGCAGATCGGAAGAGCACACGTCTG1373260.10021176655408977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)