FastQCFastQC Report
Wed 22 Mar 2023
EGAF00002207440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131935007
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT25356731.9219106874341547Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG21118451.6006706999303073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC20198841.530968956556011No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT16906501.2814263920113331Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC16127411.2223753472798922No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC15469311.1724947268923098No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA13895921.053239797076753Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC11224460.8507567669284316TruSeq Adapter, Index 7 (100% over 50bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA10736390.8137635525346203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG10035030.7606040449901216No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG9989310.7571387023915496No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT8353020.6331162736816318No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC7632850.5785310641625235No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC7605950.5764921814875108Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC7319440.5547761861262492No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT6611950.5011520558755115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT6563410.4974729716730905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG6480610.49119715436858996No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT5999210.4547094919243078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACTC5145950.3900367398320599Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA5017530.3803031594184855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4860540.36840411885527846No Hit
ACCTCAGAGGGGGCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4593330.3481509649671675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA3937350.2984310297569469Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3545340.268718672975096Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA3534350.26788568707924504Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACT3309250.25082425621882143Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA3247750.24616287017743518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC3192020.24193882068009442No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT3066590.23243186700251586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA3007180.22792889229164173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC2878790.2181975857249168Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTGAGTTCGATCCTCACACGGGGCAGATCGGAAGAGCACACGTCTGAAC2736120.2073839280578505Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2553230.1935217997146125Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2442340.18511690381007065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT2305730.17476256320659458Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2245840.17022320694612916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCGTCCGTGCCTCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2224510.16860650183616543Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2189520.1659544384607491Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT2086530.15814832222656416No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2037020.1543957169760108Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT2026760.15361806135349657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACTC2003290.1518391551682716Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG1985610.15049910142499176Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1967970.14916207947751123Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1926490.1460181072336624No Hit
TTGTGAGTTCGAGCCTCACCTGGAGCAGATCGGAAGAGCACACGTCTGAAC1888420.14313259558170183Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1867290.14153104945073447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT1836140.1391700384720486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTGAGTTCGAACCTCAGAGGGGGCACCAAGATCGGAAGAGCACACGTCT1833320.13895629686819966Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACTC1803720.13671276797673568Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1768340.1340311445922764Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC1753140.13287906218855167Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTCACACGGGGCACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1734960.1315011109977809Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCACCCAGGGACGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1732290.13129873862817923Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1725820.13080834565764643No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1723230.13061203687964332No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGAA1684790.12769848111653945Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTCC1644670.124657589929866Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCCAGCGGTGCCTCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1622870.12300526121926077Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC1616210.12250046721868138No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC1611840.12216924352761052No Hit
ATCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1562920.11846135726509643Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1550990.11755712416796249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC1550060.11748663491562934Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT1544990.11710235479807113Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC1543660.11700154758774524No Hit
TTCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1541790.11685981113412909Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1539580.11669230441621911No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCT1502660.11389395689348772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1493930.11323226746029581No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1458170.1105218420157434No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1456610.11040360197957166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT1441630.10926819445274293No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCTG1403750.10639708383082891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1400420.10614468683053922Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1376270.10431424011672656Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1375460.10425284625179122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT1352460.10250956366720775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAA1336060.10126652738950476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCCA1332380.10098760217597139Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1324870.1004183825146574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)