FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002207441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185808466
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT83590274.49873312015826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC61554453.3127903870644944No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT40679292.1893130531522713Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC40244612.165919070662797No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG27979441.505821591573766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC26490211.4256729292410175No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT21654311.1654102994424378No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA19034141.0243957344763828No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG17426440.9378711516836913No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA15270360.8218333819084434Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG14770320.7949217986655139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT14720960.7922652996876902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTC13373120.7197260861084769No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC12994380.6993427307020553No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT11670730.6281053953698751Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG10620410.571578369308533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGATCGGAAGAGCACACGT10347010.5568642927174265No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT10329580.5559262299705978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTCT10030880.5398505361967737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG9565940.5148279949741364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT8596040.4626290817125631No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC8499660.4574420198916017No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACGT8411840.4527156475206033No Hit
AGTAACGGCGAGTGAACAGGGAAGAGCCCAGATCGGAAGAGCACACGTCTG7549350.4062974181165674Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC7526410.4050628134457555No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT7288660.39226737924847843Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC7261330.3907965097779775No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGAA7248550.39010870473469167Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA6777720.3647691704209Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAAAGGTGTTGGTTGATATAGACAGCAGATCGGAAGAGCACACGTCTGA6429780.34604343593256937Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG6316000.33991992593060855No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGTC6016620.3238076353313202No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT5999640.322893791071931No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGAGATCGGAAGAGCACACGTC5935670.3194509985352336No Hit
AGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAGATCGGAAGAGCACA5777750.3109519240097488No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC5712120.3074197921638296No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGT5676900.30552429188022034No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG5570230.29978343397980584Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG5502730.29615066086385966Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC5254090.28276913927054326No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5197600.2797289118139536No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC5101840.274575217686798No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGTC5065970.2726447351435537No Hit
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA4974960.26774668060603873Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC4887440.26303645389333336No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACG4623570.24883527104733752No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGT4546920.24471005535345197No Hit
AAGGCGCCCGATGCCGACGCTCATCAGACCCCAGATCGGAAGAGCACACGT4512750.2428710648738686No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT4497530.24205194180979892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG4392380.23639288857806945Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGGTGGCCATGGAAGTCGGAATCCGCTAGATCGGAAGAGCACACGTC4376320.23552855767077913No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC4307840.2318430420710755No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGT4297710.23129785700937866No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA4296440.23122950705593792Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4156970.22372339051547843No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA3997800.21515704241377248Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGATCGGAAGAGCACA3958770.2130564922698409No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGATCGGAAGAGCACACGTCTG3879570.20879403848046407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT3873020.20844152494106485No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG3817910.20547556751262347Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG3765700.20266568478101532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGGCTCGTGCGTCGATGAAGAACGCAGATCGGAAGAGCACACGTCTGA3749250.20178036451794396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGTGAACAGCCTCTGGCATGTTGGAACAGATCGGAAGAGCACACGTCTG3651960.19654432753349352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACCAAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTC3548990.19100259941869388No Hit
AGTGCCAGGTGGGGAGTTTGACTGGGGCGGTAGATCGGAAGAGCACACGTC3454670.18592640445134506No Hit
AGGATTCCCTCAGTAACGGCGAGTGAACAGATCGGAAGAGCACACGTCTGA3451810.18577248250895093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT3427170.1844463857744781No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCTGA3400650.18301910958136858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTCGGGATAAGGATTGGCTCTAAGGGCTGAGATCGGAAGAGCACACGT3290640.17709849668529098No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT3273150.17615720480680358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGATCGGAAGAGCACACGTC3218940.17323968435324147No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT3197210.17207020050421168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGGTAAGAAGCCCGGCTCGCTGGCGTAGATCGGAAGAGCACACGTCT3136580.16880716296317735Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCAAACGAGAACTTTGAAGGCCGAAGTAGATCGGAAGAGCACACGTCTG2921220.15721673306317485Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA2866530.1542733795563438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGTC2852600.15352368282293444No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2843550.15303662213109276No Hit
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAAC2825140.15204581690050656Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG2804360.1509274609694049No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTAGATCGGAAGAGCACACGTCTGAAC2804330.15092584640357562Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2797490.15055772539449305Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCCGCCCCCCGACCCGCGCGCGGCAGATCGGAAGAGCACACGTC2786090.14994419037935547No Hit
AGCTAGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTC2772000.14918588262818983No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGGAGATCGGAAGAGCACACGTCT2748460.1479189866407917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAGATCGGAAGAGCAC2609080.14041771379782017No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG2584500.139094846195006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACAGCAGGACGGTGGCCATGGAAGTAGATCGGAAGAGCACACGTCTGAA2567810.13819660940530018Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATACCGGCACGAGACCGATAGTCAACAGATCGGAAGAGCACACGTCTGA2540210.1367112088423355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2533030.13632478942052081No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCAGATCGGAAGAGCACACGTC2503970.13476081332052975No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG2464720.13264842302718327Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGCGAAAGACTAATCGAACCATCTAGTAGATCGGAAGAGCACACGTCT2451870.13195685066362908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGATCGGAAGAGCACACGTC2447270.13170928390313497No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2432880.1309348304936762No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2411750.12979763796123261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA2372070.12766210555766605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTCTTTCTCGATTCCGTGGGTGGTGGTGCAGATCGGAAGAGCACACGTC2328760.125331210688753No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2319820.1248500700716188Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA2266970.12200574326898539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2180790.1173676338299892No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC2168250.11669274531333788No Hit
ATTAGTCAGCGGAGGAGAAGAAACTAACCAGATCGGAAGAGCACACGTCTG2136740.11499691300395322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT2122710.11424183438444618Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGAGTGTGTAACAACTCACCTGCCGAATCAGATCGGAAGAGCACACGTC2110540.11358685884635634No Hit
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCACACGT2095580.1127817286861407No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATAGATCGGAAGAGCACACGTCT2054790.11058645734688967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC2042510.10992556173409235No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGGCAGATCGGAAGAGCACACGTC2039260.10975065043591717No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA2007880.10806181457845951Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCT2001820.10773567228093903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACAC1997910.10752524053451902No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1991720.10719210178507152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT1984400.10679814772272002No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA1932030.10397965397335555Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCAGGATTCCCTCAGTAACGGCGAGTGAACAGATCGGAAGAGCACACGT1911850.10289358935884009No Hit
AGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGATCGGAAGAGCACACG1871840.10074029673115109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)