FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002207443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132223782
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC85235336.4462934512038075No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG37554522.840224310026165No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT20927421.5827273795571812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG20722151.5672029408446357Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT18095391.3685427633585612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC13624501.0304122143473404No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA11281440.8532080862730125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC10186280.7703818364535965No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT10160930.7684646321794063No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC10155570.7680592588101889No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC7845450.5933463618519095No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA7659780.5793042585939646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA7024860.5312856653880919Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG6491840.49097370395894435No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT6474120.4896335517010094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA6151970.46526955340000786No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC6110260.4621150527973856No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT5847320.442229068897757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG5647020.42708050810405646Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG5016070.37936216345710033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5008910.37882065724001146No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC4974900.37624850270883947Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT4848900.3667192033578347Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG4583830.34667212892155813No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC4496650.3400787613229819No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA4439300.3357414175310762No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA4382690.3314600394655176Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC4344720.32858839266902834No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC4184970.316506602420433Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA3900440.2949877806399457Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT3718990.28126483328089946No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT3716240.2810568525410958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA3602320.27244115585802864Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA3534810.2673354177692482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3462920.2618984230839805Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA3345790.2530399561555424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA3342770.25281155548855805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT3339870.2525922303447651Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT3252530.2459867620486003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC3216010.2432247778240075No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG3214910.243141585528086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT3211260.2428655383643466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA3068210.232046758426559Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG2885960.21826330757956988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2883630.21808709117093628No Hit
ACAAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAAC2880060.21781709435599114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG2869470.21701617943434715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT2861180.216389212040539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA2836800.21454536824547948Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2801610.2118839710695917No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG2717870.20555076846916995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT2712620.20515371432954477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC2664020.20147812743701432No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC2607520.1972050686010479No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG2532500.1915313540192036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG2513060.19006111926219144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA2468840.18671678896614832Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2459290.1859945285788301No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG2453170.185531676896067Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG2444810.18489941544706381Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC2427430.1835849771715046No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG2419930.18301775697204004No Hit
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC2414780.18262826576840768No Hit
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT2401980.18166020996132148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT2370830.1793043553995453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG2329170.17615363626491942No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA2276480.172168725290281Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT2269690.17165520193636571No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTGA2202670.16658652223395032Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT2193420.16588695065461068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC2174840.16448175714713711No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA2134650.1614422131716063No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2107330.1593760190583567TruSeq Adapter, Index 8 (100% over 50bp)
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG2104270.15914459321697516Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAGCACACGTC2067570.1563689957075952No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC1988650.15040032662202932No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1968190.14885294991788994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG1955360.1478826252300059Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1944710.14707717254676622Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC1944010.14702423199481618No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC1910420.1444838417948142No Hit
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTC1855650.14034162175152423No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1845210.13955205123386957Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT1781240.1347140410792364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACACCTGCGTTACCGTTTGACAGGTGTAGATCGGAAGAGCACACGTCT1743730.13187718378831426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1743720.13187642749471498No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1743420.1318537386867364No Hit
ACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGTCTGAA1741090.13167752227810275Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGACGTCGCCGCCGACCCCGTGCGCTAGATCGGAAGAGCACACGTCTG1737040.13137122337039187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCCAGAGTCTCGTTCGTTATCGGAATTAGATCGGAAGAGCACACGTCTG1716120.12978905716068537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCATCACAGACCTGTTATTGCTCAGATCGGAAGAGCACACGTCTGAA1690780.1278726091800944Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTG1675490.12671623626678596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT1674920.12667312753162663Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT1663100.1257791884972705Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTATCCAAGTAGGAGAGGAGCGAGCGACCAGATCGGAAGAGCACACGTC1637020.12380677479033235No Hit
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGA1604070.12131478738068467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAAC1600170.1210198328769631Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG1598630.12090336366267303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1565120.11836902381146533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG1562600.11817843782444522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGAATTAACCAGACAAATCGCTCCACCAACTAGATCGGAAGAGCACACGT1547860.11706366105909752No Hit
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA1544490.11680879011613811Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACACTCCTTAGCGGATTCCGACTTCCAGATCGGAAGAGCACACGTCTGA1542070.11662576706511088Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACCCAGGTCGGACGACCGATTTGCACGTAGATCGGAAGAGCACACGTCT1535810.11615232727195779Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGACAGCTGAACCCTCGTGGAGCCAGATCGGAAGAGCACACGTCTGAA1502450.11362933182473937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGAATCGAGAAAGAGCTATCAATCTGTCAGATCGGAAGAGCACACGTCT1491250.11278228299353894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC1489200.1126272428056853Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATAGATCGGAAGAGCACACGTCTGA1481890.11207439218460714Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTAGATCGGAAGAGCACAC1474050.11148145800276685No Hit
ATCCAATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTC1453240.10990761102265251No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC1449440.10962021945492377No Hit
CTGCTACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCT1422810.1076062096000249Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1407930.10648084472428719No Hit
AACACCTTTTCTGGGGTCTGATGAGCGTAGATCGGAAGAGCACACGTCTGA1396760.10563606477388462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC1386060.10482683062264851No Hit
ACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACT1380500.10440633138144542Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGAGCAGGATTACCATGGCAACAACACAGATCGGAAGAGCACACGTCTG1376890.10413330939210315Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT1361450.10296559207480542No Hit
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTGA1346000.1017971184639084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG1342050.1014983824921904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC1336640.10108922765497662No Hit
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT1336600.10108620248057948No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1322430.10001453445039109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)