FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002207444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141781889
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC61614764.3457426357184445No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG44685663.1517184821821633No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG14928131.052893998330069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA12352830.8712558484814658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC11362990.8014415719909049No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT9310980.6567115211732015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC8947270.6310587383978218No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG8676450.6119575681489192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA7707290.5436018700526694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT7482100.5277190234078487Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC6792440.47907670351323933No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT6686960.4716371073318116Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA6393100.45091090583508875No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC6299980.4443430712084814No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA6283140.44315533135547375No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC6047220.4265156884741464No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA5963090.42058192637001757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA5879790.4147067048880976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA5708910.4026543898000964No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG5680410.4006442600013603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT5572130.39300717738356555Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT5498240.3877956513895791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT5446960.3841788283692567No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG5374970.3791013110285193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC5372980.37896095459695844No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC5281450.37250526405385953No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA5241970.369720705301084Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC5207750.367307138925198No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5202970.36697000136597135No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT5189100.3659917381972531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT4446850.31364019984244956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG4350740.30686147791415025No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC4118960.2905138328351656No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG3898220.2749448485624281Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT3885410.27404134811604886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG3711200.2617541652305112No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG3549880.25037612526096337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT3270850.23069589656828454Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA3241320.2286131199733134Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT3224020.22739293592004545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC3222820.22730829887588816No Hit
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC3210170.22641608336872984No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTCT3201340.22579329578547228Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC3121630.2201712801273229No Hit
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC3101390.21874373531586958No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT3073500.2167766293479134No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA3050970.21518756884385987Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC3005260.21196360277016763No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT2979040.2101142833553304No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2968320.20935819242752507No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2901500.20464531968536545No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG2838070.20017154659295022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACGT2820170.19890904401760368No Hit
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG2800770.19754074513706046Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2776570.19583389807988805No Hit
AGTCCGCCCCGCCCCCCGACCCGCGCGCGGCAGATCGGAAGAGCACACGTC2773490.19561666299988426No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT2691270.18981761485770582Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT2676830.18879914909301287No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT2661750.187735543571436No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT2649680.1868842359689537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT2646670.1866719380498591Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC2503850.17659871917773645No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2411520.17008660393853264No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG2395820.1689792692774745Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT2395070.16892637112487618No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2368040.16701992170523275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG2268560.16000351074459163No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT2213230.15610103770023828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA2204380.1554768394995781Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC2200810.15522504429321013No Hit
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT2179360.15371215712889819Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC2160180.15235937503978383No Hit
GAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGGAACAGATCGGAAGAGCA2145830.15134725705340263No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA2141450.15103833184222845Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG2135050.15058693427338946Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC2110980.14888925622933405No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT2097330.14792650985204464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2058170.14516452097771104No Hit
ATACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTG2042970.14409245175171845Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACGT2039880.14387451136301338No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1950000.13753519675563075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG1918490.1353127690378No Hit
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACAC1903500.13425551129453495No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACA1872950.13210079321203005No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1867700.13173050614384182No Hit
ACCACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGT1867350.13170582033929595No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG1859860.13117754412201404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1854520.13080090927551402Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1829530.129038342830938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1816130.12809322917118138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1800980.1270246864886953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTC1789120.12618819036894058No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1768110.1247063367874863No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1759900.12412727834371004No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1750450.12346076162097122No Hit
AAGTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCAGATCGGAAGAGCACA1741990.12286407045966218No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG1714950.12095691573131742Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCGACGTCGCCGCCGACCCCGTGCGCTAGATCGGAAGAGCACACGTCTG1714630.12093434585287548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTAGATCGGAAGAGCAC1714470.1209230609136545No Hit
GGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACAGATCGGAAGAGCACACG1704170.12019659295130425No Hit
ATGCCAGAGTCTCGTTCGTTATCGGAATTAGATCGGAAGAGCACACGTCTG1693330.1194320383190832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC1684770.11882829407076105No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA1681010.11856309799906813Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1664190.11737676876346316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGACAGATCGGAAGAGCACACGT1651790.1165021859738376No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC1650530.11641331707747243No Hit
ACTCCCGACCCGGGGAGGTAGTGACGAAAAATAGATCGGAAGAGCACACGT1636050.11539203007797419No Hit
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT1597580.11267870750403107No Hit
ACCGGATAAAACTGCGTGGCGGGGGTGCGTAGATCGGAAGAGCACACGTCT1595850.11255668909870428Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGGCAGGGACTTAATCAACGCAAGCTTAGATCGGAAGAGCACACGTCT1570290.11075392005815356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1542860.10881925829045767No Hit
AAAGGATTTAAAGTGGACTCATTCCAATTAGATCGGAAGAGCACACGTCTG1536320.10835798639980032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACACCTGCGTTACCGTTTGACAGGTGTAGATCGGAAGAGCACACGTCT1530570.10795243389654655Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTAGATCGGAAGAGCACAC1501030.10586895199287408No Hit
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG1496540.10555226838598546Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCACAC1495290.10546410479832159No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1491300.10518268662649853Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTGCTCAATCTCGGGTGGCTGAACGCCACTAGATCGGAAGAGCACACGTC1463730.10323815053698432No Hit
ACGGAATCGAGAAAGAGCTATCAATCTGTCAGATCGGAAGAGCACACGTCT1459730.10295602705645995Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAGATCGGAAGAGCACACGTCT1443860.1018367021474795Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1442480.1017393695466986No Hit
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCACACGT1431030.10093179108369757No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG1427930.1007131453862912No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA1423970.10043384314057206Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)