FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002207445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197246707
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC85501694.334758805377673No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG71195083.609443274508No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA34788331.763696364269341Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA34569771.7526158244051193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT16850770.8542991797576626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG14559530.7381380516532526Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA14499790.7351093572375837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA10557940.5352657167554133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG10120760.5131015951510917Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC9613210.48736986012141637No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA9119980.46236411946791084Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG8439790.42787989357916123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT8144160.4128920641498973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG8118820.41160737857083723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA7851910.39807559372841644Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG7433600.37686814208766484No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC7382660.3742855894674074No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA7337100.3719757917175266No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT7248150.36746621072867897Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC7204740.3652654135310862No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT6934610.35157038134988994No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC6895540.3495896131741251No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT6814640.3454881505322165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT6720430.34071189842474786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC6592140.3342078608186853No Hit
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTCT6541470.331638996639878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA5882550.29823311574981076Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT5720840.29003475327981015No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT5593740.28359104621199077No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC5411430.27434830635727675No Hit
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG5380840.27279745663890853Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC5263110.2668287891873399No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC5260820.26671269092467026No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACGT5190680.2631567380235149No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC5131740.2601686019528833TruSeq Adapter, Index 11 (100% over 50bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5028130.254915789291225No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT4969170.2519266392619675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG4832610.24500332976408068Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4778040.24223674365321599No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC4759010.24127196202063844No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC4754080.24102202121934535No Hit
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC4710110.23879283318022643No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG4654600.23597859101394275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA4596370.23302645047453188No Hit
GGAAGGACCCCACACCCCCGCCGCAGATCGGAAGAGCACACGTCTGAACTC4433750.22478195288705125Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA4353960.220736764948882No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG4272550.21660944636201201Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT4112000.2084698934923157No Hit
ATACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTG4013660.20348425892859137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC3992480.20241047674372584No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT3933680.19942943838347577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG3910330.19824564168769623Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA3593970.18220684414265026Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGCAGGATCAACCAGGTAGGTAGGCAGATCGGAAGAGCACACGTCTGA3571280.18105650808152657Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3507480.17782198006479266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT3475430.17619711136673122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTCT3387050.17171642819872274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC3384270.1715754879497177No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT3374970.17110399718865776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT3366500.17067458571057412Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGACCTCTAATCATTCGCTTTACCGGATAGATCGGAAGAGCACACGTCTG3239510.16423645541519738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAGGGGGCGCCGAGAGGCAAGGGGCAGATCGGAAGAGCACACGTCTGA3142150.1593005048241439Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGCACACGTCTGAACTC3135380.15895727982926477Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGCGGAGGAGCGAGGAGGAAGGACGCAGATCGGAAGAGCACACGTCTG3126020.15848274719232702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA3105940.15746473273188788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC3101800.15725484329631925No Hit
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCAGATCGGAAGAGCACACGTC3090270.15667029614846753No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG3066910.155485992473375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2995600.15187072299260235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT2990250.15159948906016463No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC2925930.14833859811915642No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA2905070.14728103927230582Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGGTGGTGAGGTTGATCGGGGTTTAGATCGGAAGAGCACACGTCTGAA2887640.14639737432980313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC2847120.14434309415365804No Hit
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA2835570.14375753304718036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG2774760.1406745918450238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT2742310.13902944397444364Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGATTTAAAGTGGACTCATTCCAATTAGATCGGAAGAGCACACGTCTG2713720.137579990118669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT2665810.1351510522302408Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG2650210.1343601645020112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACACG2624410.13305215787455452No Hit
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG2523740.12794839713090875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG2515460.1275286182597715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGGGGGGGGCGGCCCGCCGGCGGGGACAGATCGGAAGAGCACACGTC2499500.12671947927627508No Hit
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC2495660.1265247992200955No Hit
AATGGGGCGAAGCTACCATCTGTGGGATTAGATCGGAAGAGCACACGTCTG2479620.12571160440209528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG2474020.12542769598683337No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT2448870.12415264301471962Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC2429780.12318481950626431No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGTC2408260.12209380002475782No Hit
ACCGGATAAAACTGCGTGGCGGGGGTGCGTAGATCGGAAGAGCACACGTCT2384610.12089479394958923Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGGGGAGTTTGACTGGGGCGGTACAGATCGGAAGAGCACACGTCTGAA2351540.11921821336160507Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTAGATCGGAAGAGCAC2351330.11920756679603274No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2349130.11909603134717985No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCAGATCGGAAGAGCACACGTC2337960.11852973545459493No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG2335640.11841211625398644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG2323340.11778853169903618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC2315690.11740069252461588No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT2278020.11549090145266659Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC2226780.11289313945302011No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG2176620.11035013121917417Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCTGAAC2176490.11034354048810559Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGGGGGACCGGCTATCCGAGGCCAACAGATCGGAAGAGCACACGTCTG2157360.10937368906239839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAGATCGGAAGAGCACACGTCTG2150320.10901677562606914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC2141620.10857570362378724No Hit
CGTGCGGAGGAGCGAGGAGGAAGGACGCGCAGATCGGAAGAGCACACGTCT2130630.10801853335883574Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2125160.10774121567464243Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTCTCTTCACCGTGCCAGACTAGAGTCAGATCGGAAGAGCACACGTCTG2096970.10631204099138648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC2064910.10468666531401205No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCCAGATCGGAAGAGCACACGTC2039610.1034040076522038No Hit
ATGCCAGAGTCTCGTTCGTTATCGGAATTAGATCGGAAGAGCACACGTCTG2015280.1021705269837534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGGCAGGGACTTAATCAACGCAAGCTTAGATCGGAAGAGCACACGTCT1991700.10097506976377557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGCGGAGGAGCGAGGAGGAAGGACGCAGATCGGAAGAGCACACGTCTGA1989620.10086961806667828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)