FastQCFastQC Report
Sun 5 Mar 2023
EGAF00002207446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170855544
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC37197402.1771257244072806TruSeq Adapter, Index 12 (100% over 50bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT37089832.17082976248052Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC33766761.976333878870211No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC31243591.8286553230019857No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC27238071.5942163398572538No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG23177511.3565559218845131Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA21939551.2840993910036655Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG17513341.0250378530298085No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA15334110.897489753098091No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT12609800.7380386790375383Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC11268940.659559516546914No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT11187210.6547759433548144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG11185580.6546805411242611Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC10785830.6312835830483792No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT9546410.5587415998628642No Hit
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA8827020.5166364399624047Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA8595020.5030577175769023Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA7781830.45546253974644224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA7780790.4554016696116106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTC7334420.42927609068395234No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC6825920.39951410648986607No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG6612300.3870111466795599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG5896160.345096205950449Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG5687030.33285604124148294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG5547600.3246953461457475Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT5400770.3161015366290953Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG5338230.3124411344825896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC5225260.3058291160865111No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA4921150.2880298692561009Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT4864790.2847311761800366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG4814280.28177487761239983Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC4723740.27647566414350594No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGT4720500.2762860302619153No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT4435520.2596064427385511Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC4418800.25862783826318214No Hit
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGAA4319880.25283815197708776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT4115680.24088653511881358No Hit
ATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCTGA4075020.2385067469628027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT3974540.2326257554744609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT3887460.22752905226183354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA3877460.22694376250383774Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT3785190.22154329390681052No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC3691080.2160351319943121No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT3683180.21557275308549542Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3675340.2151138859152267No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC3651510.21371914042192275No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT3580610.20956943603773256No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG3544000.20742669023370996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTCCCTCAGTAACGGCGAGTGAACAGATCGGAAGAGCACACGTCTGA3482550.2038300846708258Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGATCGGAAGAGCACACGT3446880.20174235610405478No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC3445950.20168792415656117No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG3312100.19385382074578744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA3282110.19209853676155805Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT3254500.19048255173973166No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT3174430.18579613664745934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGTC3137980.18366275547956465No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG3131930.1833086551759772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAGATCGGAAGAGCAC3028850.17727548835055654No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG3004890.17587313409039862No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2944610.17234500742919995No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTG2943900.17230345185638227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT2894810.1694302644343809No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG2855760.16714470792940733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC2854670.1670809113457858No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGAGATCGGAAGAGCACACGTC2788370.16320044025027364No Hit
ATAGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGA2760660.1615786023308673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2722190.15932699263185748Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGT2716510.15899454804931587No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGT2676060.15662705097822285No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2664610.1559568942053177No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGTC2662080.15580881589654474No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGATCGGAAGAGCACACGTCT2617690.1532107146608014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2595400.15190610379022879No Hit
GGAAGGACCCCACACCCCCGCCGCAGATCGGAAGAGCACACGTCTGAACTC2592520.15173754033992598Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCTGA2584290.15125584686909546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT2549400.14921377090344812No Hit
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAAC2528900.14801392689955675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGTC2519490.1474631692372827No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2513190.14709443668974534No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2471550.14465729013745085No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCAGATCGGAAGAGCACACGTC2413400.1412538301947053No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGATCGGAAGAGCACACGTC2371670.13881141603458885No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT2312070.1353230890769339Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAACGGCGAGTGAACAGGGAAGAGCCCAGATCGGAAGAGCACACGTCTG2275450.13317975798315332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2240560.13113768201750597No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTAGATCGGAAGAGCACACGTC2196200.12854133665103662No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2194310.1284307168867754No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG2178080.12748079160954826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2162250.1265542779226409No Hit
AGCTGCGAGAATTAATGTGAATTGCAGATCGGAAGAGCACACGTCTGAACT2157250.126261633043643Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGGCAGGATCAACCAGGTAGGTAGGCAGATCGGAAGAGCACACGTCTGA2149500.12580803348119626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG2122930.12425291859420141Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA2038110.11928849086688109No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1988400.11637901547988398No Hit
AAATACCGGCACGAGACCGATAGTCAACAGATCGGAAGAGCACACGTCTGA1956210.11449496774889553Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1908170.11168323575148373No Hit
AGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGATCGGAAGAGCACACGTCTG1884990.11032653409244948Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1848720.10820368814019872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCT1837720.10755986940640333Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1834500.10737140610432869Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGATCGGAAGAGCACACGTC1832490.10725376286297154No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACG1820910.1065759973232124No Hit
AGCTAGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTC1806060.10570684203258865No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC1801730.10545341156737648No Hit
AAGGAGTGTGTAACAACTCACCTGCCGAATCAGATCGGAAGAGCACACGTC1790510.10479671645890519No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1786120.10453977425514505No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1723070.10084952233098154No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT1721920.10078221400881204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)