FastQCFastQC Report
Tue 23 May 2023
EGAF00002207449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107221310
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC34530483.220486673777815No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT34034123.1741936374401694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT25722692.3990277678942737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC25213002.3514915085443366No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG24698542.30351037494319No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC20112061.8757521242745496No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT13693921.2771640264421318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT9942330.9272718268411383Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT9588430.894265328412794No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG8519910.7946097655400778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG8395500.7830066616421679Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC7038010.6564002995300096No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC6987020.6516447150291299No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5525090.5152977519114438No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5315880.49578577243646804No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC5287420.49313144933595754Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT5033460.46944585922332044Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA4847140.45206871656389946Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCTGA4831960.4506529532235709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC4563480.4256131546984457No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4235940.3950651227820291No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAGATCGGAAGAGCACACGT4035550.376375740979102No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG3914430.3650794790699722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3801450.35454239460420695No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT3642690.33973563650733235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG3441910.32100988133795416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC3435730.32043350337726706No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC3405660.3176290235588429No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG3133060.29220497305992627Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT3123770.29133854081805194No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA3050560.2845106070798799Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC3005360.2802950271732364No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA2783280.2595827266053735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC2737910.2553512916415589No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA2714360.2531548998981639No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA2645850.24676531185824907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2535790.23650056131565636No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2531340.2360855318779448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA2506840.23380053834447648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT2500480.23320737267619654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGT2493280.23253586437248344No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2426750.22633094111608973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG2400100.22384542774192928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGAA2380810.22204634507823118Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTCTTAGAGGGACAAGTGGCGTTCAGATCGGAAGAGCACACGTCTGAA2343650.21858061611073396Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2304020.21488452248904624No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG2299780.21448907871019296Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA2204690.20562050584907052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA2176430.20298483575699644No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2168390.2022349848178501Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2154840.20097124349627887No Hit
AGTCCGCCCCGCCCCCCGACCCGCGCGCGGCAGATCGGAAGAGCACACGTC2154730.20096098434163878No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG2145650.2001141377586228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2073520.19338693026600776No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT2073190.19335615280208757No Hit
ATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTGAAC2072890.19332817328943286Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2062510.19236008215157976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT2050120.19120452827894008No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC2022160.18859683769952074No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGAGATCGGAAGAGCACACGTC2018370.18824336318964952No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG1983450.18498654791664082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA1903230.17750482623277034Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTAGTAGCGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA1903090.1774917691268648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1878800.1752263612522548No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1822280.1699550210681067Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1805950.16843200292926844No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAAGATCGGAAGAGCACACGTCTGAA1749910.1632054299653679Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1718700.16029462799885583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1667120.15548401712308868No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1661530.15496266553728918Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1654440.15430141638821612No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1652660.15413540461313147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC1637280.15270098826436648No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC1597930.14903100885448983No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1575400.14692974745412082No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1572100.14662197281491898Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGTC1518620.14163415835900534No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT1499770.13987611231386746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC1469660.137067901893756No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT1459220.13609421485337195No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC1456140.13580695852345023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1444370.13470922897696364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT1422260.1326471388943112Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1403140.1308639112877841Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA1378980.12861062786865785Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1349060.12582013780656104No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC1345940.125529150874952No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1292260.12052268341060186No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1266790.11814722278621667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT1241910.11582678853671906No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG1238770.1155339363042664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACGTC1211130.11295609053834542No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACG1207210.11259049157299048No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT1178510.10991378486235619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1143690.10666629609356573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACG1132190.10559374810846836No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1130690.1054538505451948Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAAC1125680.10498659268386107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTAGATCGGAAGAGCACACGTCT1124600.1048858664383041Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1113480.10384875916923603No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1103630.1029300985037396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT1099670.10256076893669738Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGAGGTGTAGAATAAGTGGGAGGCCCAGATCGGAAGAGCACACGTCTG1089850.1016449062224664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGTACGGCCCGGCGCGAGATTTACAGATCGGAAGAGCACACGTCTGAA1088260.10149661480539643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA1073980.10016479000303205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)