FastQCFastQC Report
Sat 13 May 2023
EGAF00002207451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78380906
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1231888515.716691256413903TruSeq Adapter, Index 1 (100% over 50bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC57349747.3167998338779086Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA46883315.981470793409813Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA22716682.898241569190333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT21136782.696674621240025Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT20144362.570059600995171Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC12600901.6076491894594838Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA8500181.0844707510780751Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC6846330.8734691073869445Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT5658970.721983234028961Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC5513480.7034213153902559Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA5436390.6935860118789645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAAC5028020.6414853127622689Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGA4051990.5169613630135891Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3654040.46619006930080653No Hit
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT3618310.46163156113556536Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT3349750.4273681143721406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC3004220.38328467394852517Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAGATCGGAAGAGCACACGTCTGA2988130.38123187808010284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG2952930.37674098842388987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA2863880.36537980308622614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA2766310.3529316183204108Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2740730.3496680683941061Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACT2739450.34950476331569835Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC2630030.33554473075368635Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAAGATCGGAAGAGCACACGTCTG2384010.304156984355348Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTG2359490.3010286714470996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCAGATCGGAAGAGCACACGTCTGAAC2217540.28291839341586583Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG2165700.2763045377403522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT2042050.26052901200197914Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1896190.24191988798904673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1825040.2328424221072413No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGTC1793850.22886313664197758No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1732140.22099004571342926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1663200.2121945362560622No Hit
ATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTCC1656980.21140097564067453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGAAGATCGGAAGAGCACACGTCTGAA1624100.20720607643907563Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1599660.20408797009822777Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAACT1546190.19726615561192926Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAAC1520900.19403960449245125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC1498700.19120728203881696No Hit
TAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTG1467630.18724330642465398Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG1375930.1755440285418492TruSeq Adapter, Index 1 (100% over 51bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1375200.17545089361431981No Hit
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA1359860.17349378431527698Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAAC1314260.1676760408970011Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCACCCAACTTACACTTAGGAGATAGATCGGAAGAGCACACGTCTGAA1312750.16748339193731698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGATCGGAAGAGCACACGTCTGA1280430.16335993870752144Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACAAAGCACCCAACTTACACTTAGGAGAAGATCGGAAGAGCACACGTCTG1235350.15760853797734872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGATCGGAAGAGCACACGTCTGAA1230000.15692597378244136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGAGATCGGAAGAGCACACGTCTG1228020.15667336123927936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1207630.15407196237308102Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1180680.15063362498004298TruSeq Adapter, Index 1 (100% over 51bp)
CACAAAGCACCCAACTTACACTTAGGAGATAGATCGGAAGAGCACACGTCT1169310.14918301658824917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1147720.14642851921104358Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1144340.14599729173837311Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCAAACATATAACTGAACTCCTCACACAGATCGGAAGAGCACACGTCTG1138620.14526752216923852Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1127440.14384115437502087Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGCTCATAAGGAAAGGTTAAAAGATCGGAAGAGCACACGTCTGAACTC1121870.14313052211976218Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1120700.1429812510715301Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1115400.14230506598124804No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA1057900.13496909566215015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGAACTAATGTTAGTATAAGTAACAGATCGGAAGAGCACACGTCTGAA1053480.13440518281327343Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA1047320.13361927712343616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACTC1041130.1328295439708237Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1039320.13259862038338777TruSeq Adapter, Index 1 (98% over 50bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTGA1025460.1308303325812539Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTGA1019510.1300712191308429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGATCGGAAGAGCACACGTCT998720.12741878742764215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCTG985750.12576404768783866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACGGAGATCGGAAGAGCACACGTCTG920780.1174750391377206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC915140.11675547613598648Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGAAGATCGGAAGAGCACACGTCT912720.11644672747212184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAGATCGGAAGAGCACACGTC908810.1159478814904232No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAA908650.11592746835562223Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTATTACCCTCACTGTCAACCCAAAGATCGGAAGAGCACACGTCTGAACT901130.11496805101997673Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTC884040.11278767305904834No Hit
CCGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCT876030.11176574049807488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAAGTATAGGCGATAGAAATTGAAAAGATCGGAAGAGCACACGTCTGAA871500.11118779361902248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAAAGCACCCAACTTACACTTAGGAGATAGATCGGAAGAGCACACGTCTG862470.11003572732369285Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC845240.10783748786981359TruSeq Adapter, Index 1 (98% over 50bp)
CCCTTGTAAATTTAACTGTTAGTCCAGATCGGAAGAGCACACGTCTGAACT814980.10397685375058052Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG811070.10347800776888189Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAAGATCGGAAGAGCACACGTCTG798210.10183730205925408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)