FastQCFastQC Report
Tue 23 May 2023
EGAF00002207452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94119110
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA76305158.10729616971516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC59722676.345435055643854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG16765131.7812673749252412No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT13913911.4783299587087044Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC13501091.4344685154800125TruSeq Adapter, Index 2 (100% over 50bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT12501281.3282403541639949Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC8992620.9554510237081503No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT7296970.7752910115703389No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG5988530.6362714224560773No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT5861580.6227831946137188No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG5670270.6024568230617565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA5154540.5476613622886999Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4768500.5066452498328978No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT4601600.4889124004678752Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA4595230.488235598487916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA4248420.4513876087438566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCAC3615440.38413452910891316No Hit
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT3528820.37493129716164975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG3502980.372185839836352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC3411250.36243967882824224Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTCT3405780.361858500361935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT3280650.3485636445138506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC3106580.3300689944900669Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC3092310.32855283055694Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT3031230.3220631814304236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC2695840.2864285478262597Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG2657120.28231461177225325No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT2464080.2618044305773822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA2365760.25135809295264266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGAAGATCGGAAGAGCACACGTCTGAA2334950.24808458133528888Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2332330.247806210662213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2243190.23833523287672395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT2209570.2347631634000789Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC1999810.21247650981825053No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1993460.21180183280526135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1931400.20520806029721275Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1921250.2041296395599151Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGAGATCGGAAGAGCAC1863970.1980437341577072No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1808690.19217032545250373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTC1808370.192136325980983No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA1747640.18568386377644241No Hit
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA1745810.18548942929868334Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1674350.17789692231471377Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1666160.1770267483404805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAGAACTAATGTTAGTATAAGTAACAGATCGGAAGAGCACACGTCTGAA1655730.17591857806560218Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC1651320.17545002284870734Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG1586120.16852263052636174Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1568100.1666080352863515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1562730.16603748165489451No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC1425870.1514963326788789No Hit
TAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTG1384180.14706683902982082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCAGATCGGAAGAGCACACGTCTGAAC1325280.1408088113030393Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGT1305640.13872209373845545No Hit
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA1291260.13719424248699333Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTC1259990.13387185662932852No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAAC1245760.13235994263014175Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACGT1213040.12888349666714868No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1192330.12668309336966743Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCACACGT1170920.12440831622823462No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1151420.12233647343244107Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTATTCTCAAACTTTAAATGGGTAAGAAAGATCGGAAGAGCACACGTCT1147140.12188173050085152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC1131510.12022106881376163No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1117620.11874527925306562Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGTTAGTATAAGTAACATGAAAACAGATCGGAAGAGCACACGTCTGAAC1108830.117811356269731Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTATTCTCAAACTTTAAATGGGTAAGAAGAGATCGGAAGAGCACACGTC1093100.11614006974779084No Hit
CCAAAACATCAGATTGTGAATCTGACAACAGATCGGAAGAGCACACGTCTG1091870.11600938427913311Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGCATTAAAAATTTCGGTTGGAGATCGGAAGAGCACACGTCTGAACT1081700.1149288385748654Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1072870.1139906656575907Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTCTG1063620.11300786843394502Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1034320.1098947918228296Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT1011680.10748932921273906No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGT991390.10533355022162876No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA988590.1050360548458225No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC977710.1038800728141182No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGTC955600.10153092182873383No Hit
TGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCAGATCGGAAGAGCAC954150.1013768617234056No Hit
AAATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTCT945990.10050987519962737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG943020.10019431760457573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)