FastQCFastQC Report
Tue 23 May 2023
EGAF00002207453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113377930
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA95286258.40430320080813Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC85556987.546175873911263Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG76311116.730684710860394No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT24172542.1320322217913135No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT19987011.762866018104229No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC19813191.747534992039456No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT16486241.4540960484990333Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC15175871.3385206450673424No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT14458161.2752182016376556Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC8249060.727571935737405TruSeq Adapter, Index 6 (100% over 50bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7663420.675918143857451Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG7483780.660073790375252No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA6017670.5307620274951219Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT5879310.5185585942519854Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT5103540.45013522473024514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA4976690.4389469802456263Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT4939460.4356632723846696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA4875360.43000961474600924Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA4586200.40450553295513514Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG4330300.3819350026940869Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG3863300.3407453284779498Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG3636130.32070880108677235Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG3341620.29473284615445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC3341100.29468698184911296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC3265050.28797932719357283No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG3256330.28721021807330577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA3197390.28201167546452827Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC3161000.27880205609680825Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC3051250.2691220416530801Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTCT3050940.2690946994710523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC3039680.26810156085933123No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2999480.2645558972544304No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA2901100.25587872348701374No Hit
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCAC2887130.2546465612840171No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG2706270.23869460308545057No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC2681260.23648870639991398Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTC2428540.21419865400611918No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT2270080.20022238896053227Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT2191030.1932501325434324No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2117370.18675327729126823No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1991510.17565235138796415Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1987840.17532865523298935No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1977560.1744219531967112Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1974790.17417763757020438No Hit
GTTTAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1967060.17349584703125204No Hit
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1961530.1730080977841102Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1954120.17235453143305757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1946640.17169479104090188Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1926670.1699334253147857No Hit
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT1923270.1696335433183513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1910910.16854338406072505Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG1857560.1638378827343205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1830730.16147146098010434No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1742190.15366218099060375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCTGCATTAAAAATTTCGGTTGGAGATCGGAAGAGCACACGTCTGAACT1712210.1510179273867498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1705950.15046579171096175No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGAGATCGGAAGAGCAC1701960.15011387136808724No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1664710.14682839949538679Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGAAGATCGGAAGAGCACACGTCTGAA1617620.14267503384477032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGTC1608950.14191033475386258No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCAGATCGGAAGAGCACACGT1546190.13637486590203227No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTCT1503150.13257871262952148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1467240.12941142954365104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTC1459230.12870494284028647No Hit
ATTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAAC1370140.12084715252783323Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1358100.11978521745810669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT1346760.11878502279941078Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1330080.11731383700513848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1269930.11200857168586514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCTGCATTAAAAATTTCGGTTGGAGATCGGAAGAGCACACGTCTGAAC1261420.11125798468890725Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1258460.11099691095083496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTCT1256310.11080727968838379Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAAC1197460.10561667513245303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGT1196290.10551348044544472No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1168180.10303416193962969Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)