FastQCFastQC Report
Sun 14 May 2023
EGAF00002207454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75111769
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC40015845.32750599975884TruSeq Adapter, Index 12 (100% over 50bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC31607434.208052935086644Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA21193932.821652356503546Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG13318591.7731695282000348No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT12905421.7181621697659657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT8291431.1038789407289822Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC8287511.103357051809018Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG7156150.9527335190308193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC6569130.8745806532662012No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT5726610.7624118132539257No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG5614860.7475339849870931No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC4821820.6419526612400781No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT4071090.5420042763205324No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT3600250.4793190265562778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTCT3171480.4222347632366374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT3016390.40158686716591646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC3015070.4014111290602143No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG2998550.3992117400403657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC2904720.38671968969336884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2555710.34025426827585437No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2522500.3358328572983017Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA2383540.31733242762528996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG2272770.30258507158844844No Hit
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT2265130.3015679207342328Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC2074930.2762456573216908Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2059550.2741980421204033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1765700.23507634336238306Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAAC1542460.20535530191014406Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1523740.20286301604745854Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGTTAGTATAAGTAACATGAAAACAGATCGGAAGAGCACACGTCTGAAC1513250.20146643064684042Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA1484050.19757889073282242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT1441010.19184876340750276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGAA1400560.18646345554715932Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1395960.18585103487577292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1355870.1805136555897119Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT1293180.1721674269181438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA1266990.16868062313909823Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1238190.16484633719650513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTC1214610.161707015581007No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1199920.15975126348042742Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC1187970.15816030108410842Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1181200.1572589776177419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTCC1175050.156440197807084Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACGT1097060.1460570047285133No Hit
CCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGT1087960.14484547687859675No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA1087890.1448361574335974Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCAC1076600.14333306409012947No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1065070.1417980183638066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGTACCGCAAGGGAAAGATGAAAAATTAGATCGGAAGAGCACACGTCTG1034040.13766684153051967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT1032340.13744051215196382Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAGATCGGAAGAGCACAC1015880.13524911122782904No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGTCTGAA983360.1309195633509843Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAGAACTAATGTTAGTATAAGTAACAGATCGGAAGAGCACACGTCTGAA948620.1262944559327314Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC943770.12564875152920443No Hit
AAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTCTGAAC940840.12525866618851703Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCACACGT937760.12484861060854525No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA903310.12026211231957538Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACG884910.11781242963402978No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAGATCGGAAGAGCACACGTCTGAAC877590.11683788195695403Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG873540.1162986854962769Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGCATTAAAAATTTCGGTTGGAGATCGGAAGAGCACACGTCTGAACT867160.11544928465204968Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACT866240.1153268005177724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG861160.11465047508067609Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGCGCTGGAGCGTCGGGCCCATACAGATCGGAAGAGCACACGTCTGAACT854870.11381305638001948Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTC846770.11273466345866519No Hit
TGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCAGATCGGAAGAGCAC827880.11021974465812408No Hit
GCATGCTCATAAGGAAAGGTTAAAAGATCGGAAGAGCACACGTCTGAACTC817360.1088191652096491Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG811320.10801503024113306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA804920.10716296669833458Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC800870.10662377023765743Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG790090.10518857570775625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGT788350.10495692093205793No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA779560.10378666490999566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC779450.10377202006785383No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT774940.10317158154003801Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC764580.101792303680133No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT761490.10138091675087563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGAAGATCGGAAGAGCACACGTCTGAA757960.10091095045305083Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA757750.10088299211805277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG753640.10033580756166188No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)