FastQCFastQC Report
Sat 13 May 2023
EGAF00002207455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79962894
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA1027468312.849313582872576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC61907417.742017191123674Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG61297497.6657418126962735No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT16968992.12210803676015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT12467671.5591819375621898No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC8746451.0938135880874948No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC8444761.0560848385502406No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT5725060.7159645822723725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC5707200.7137310463025512No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA5664960.7084485961701187Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG5095450.6372268117259489No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4358190.5450265469381336No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4109870.513972143129287No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG3042060.3804339547790754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC3013510.3768635487354922Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA2957750.36989031437506503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT2955120.36956141182183827No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA2724510.3407217852820585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT2679480.33509042331559435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2674230.3344338687891911TruSeq Adapter, Index 1 (100% over 50bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC2581450.32283098708258356No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTC2445190.30579058331730713No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC2321610.29033591505580075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA2227320.2785441957615991Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA2171910.2716147317029321Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2133150.266767483428001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2117880.26485784769120535Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2059980.2576169892000157Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2054430.2569229172721037No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2053050.25675033722516344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1987630.24856904253615433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1893790.23683359934421586No Hit
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1814410.2269064949049993Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG1796120.22461918399301556No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1731960.2165954623903432No Hit
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC1674470.20940587768121546Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1643430.20552407720510965Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTATAGGTAGAGGCGACAAACCTACAGATCGGAAGAGCACACGTCTGAAC1610940.20146094262171152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1523300.19050085906095396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1510400.1888876107960775No Hit
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACG1459060.18246713281787924No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1449710.1812978404708564No Hit
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1435440.17951326273908996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACG1415630.17703586365946183No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1381770.17280139960917373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTG1360130.17009514438034223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1280020.16007674759745438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1256240.15710286823786043Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1182730.1479098542881652Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC1143420.14299382411046804No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA1123320.14048015820938145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1108310.13860303755389342No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1093300.1367259168984054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1082110.13532651782212884Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1035870.12954383566957944No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT1028660.12864216745331905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1001450.12523933913647498No Hit
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGT1001040.12518806535441301No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG967070.12093984492357168No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAA937380.11722687275425524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG934170.11682543655811156No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA894050.11180810939634075Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT871010.10892677296046839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA858750.1073935618188106Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG851280.10645937852124261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAA835110.10443719057992072Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA832990.10417206760925887Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCAGATCGGAAGAGCACACGTC825540.10324038547179146No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)