FastQCFastQC Report
Tue 23 May 2023
EGAF00002207456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100281980
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA52290945.214390461775884Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC43725754.360279882786519No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC34385923.4289231225789516Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG19333081.9278717871346378No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG14953771.4911721926511623No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT14451271.4410634891732292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG8060040.8037376206572706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG6554900.6536468466218956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC5571080.5555414841230698No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT5268490.5253675685302583Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5186500.5171916230612918No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT5168950.5154415578950475No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA4783100.47696505394089744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT4412460.44000527313082566Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA3963410.39522654020193854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT3850250.38394235933514675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3785940.3775294424781003No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC3783830.3773190357828994No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA3630600.3620391220835488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA3504310.34944563320349276Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT3398850.3389292871959648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA3248020.3238886986475536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC3233210.32241186302863184No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2996640.29882138346291126No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC2827120.28191705030155967No Hit
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG2747930.274020317508689Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT2718340.2710696378352322Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2654190.26467267598824834No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2521830.2514738939139415Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2471580.2464630235661482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC2462760.24558350363644596Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA2411960.24051778794156237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT2374990.236831183428967Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2244070.22377599644522375No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2243320.2237012073355552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2055500.20497201989829078No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT2042230.2036487512512218No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1981300.1975728839817483No Hit
TCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAA1945920.1940448323816502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1919920.19145214324647358No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1881880.1876588396040844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGTACCGCAAGGGAAAGATGAAAAATTAGATCGGAAGAGCACACGTCTG1881310.1876019998807363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA1800450.17953873667033698Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1777130.17721329395370933No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1760080.17551308819391082No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC1753570.17486391872198773No Hit
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC1743930.17390262936571454Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACACGTCTGAA1667410.16627214580326397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAGATCGGAAGAGCACAC1652930.16482821739259634No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1615080.16105386032465652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1613050.16085143113448697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG1608760.16042363742718282Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1556930.1552552113550211Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG1544560.15402168963955437Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1536360.15320399537384483No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGTCTGAA1531660.1527353169532552Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1515460.1511198721844144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1489730.14855410712871844No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG1481880.14777131444752087No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1476320.14721687784784465Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGA1470070.14659363526727334Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT1454940.14508488962822633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1452170.14480866851651714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCAATAAACGAAAGTTTAACTAAGCTATAGATCGGAAGAGCACACGTCT1447870.14437987762108406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1431110.14270859031702404Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC1415130.14111508368701936No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTGA1401630.13976887971298532Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA1398680.13947470921495567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG1374820.13709541833936664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1356090.13522768497391055Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGA1332120.1328374250289035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1319380.13156700735266694Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA1312200.13085102627610662No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA1294650.1291009611098624Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA1281670.12780661091853193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1261290.12577434151180503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGAAC1260950.12574043711542193Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1255880.12523486273406248No Hit
AGAAGAACTAATGTTAGTATAAGTAACAGATCGGAAGAGCACACGTCTGAA1254720.12511918891110846Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG1247440.124393235953259No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1244130.12406316668258843No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT1222250.12188131905652441No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC1204630.1201242735733778No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA1183660.11803317006704496No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1181900.11785766495635606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1178870.11755551695329511Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1177850.11745380376414587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT1149300.11460683165609613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCTG1112010.1108883171233755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1104190.11010851600656468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT1102830.10997289842103239Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1092110.10890391274683646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1073980.10709601066911524No Hit
TAGCAATAAACGAAAGTTTAACTAAGCTAGATCGGAAGAGCACACGTCTGA1067630.10646279620725478Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA1059080.10561020035703324Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC1055980.10530107203706987No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1055880.10529110015578073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1055360.1052392463730772Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1039380.10364573974307248No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC1033590.10306836781643124No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1028010.10251193684049716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1026580.10236933893806245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1005820.10029917638243681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)