FastQCFastQC Report
Sat 13 May 2023
EGAF00002207460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77953999
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA45185915.796483898151267Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC44425665.6989584331651795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT21763312.7918144391797015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG15213661.9516202112992302No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT14663281.8810170341613905Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG8658411.1107076110360932Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC7880901.010968019742002TruSeq Adapter, Index 4 (100% over 50bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA7872801.009928945402788Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC6817270.8745247309249651Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT5866950.7526169375864861Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT5854510.7510211246507059Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGAA5808110.7450688963371848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC5622680.7212817908161453Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT5318270.6822318377790985No Hit
ATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACTC4696620.6024860892640029Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA4462520.5724555580528973Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT3569050.4578405272063079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT3480280.4464530421332201No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2632600.3377119883227543No Hit
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC2605780.334271497732913Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT2583350.331394159778769Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTC2578680.3307950885239383No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAA2558950.32826410868286565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2477340.3177951140133298Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGTTTCTATCTACTTCAAATTCCAGATCGGAAGAGCACACGTCTGAACT2471230.3170113184315278Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG2430160.31174282668936587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2352310.3017561677624775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2334150.2994265887501166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA2314570.2969148510264368Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA2213470.28394566390365683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG2095480.2688098143624421No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA2046370.2625099451280235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC2029500.26034584832524116Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA2005740.25729789693021393Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTCC1938390.24865818622082492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1930340.24762552592074205No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA1912440.24532929991186214Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG1838760.2358775718484949No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC1831430.2349372737118977No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1805030.23155066105075636No Hit
CCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAAC1782690.2286848683670481Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC1727250.22157298177865126No Hit
TCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTC1623450.20825743654280005Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTTCAACTTTAAATTTGCCCACAGAGATCGGAAGAGCACACGTCTGAACT1556960.1997280473064634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC1528210.19603997480616742No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG1521230.19514457494348686No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC1506410.19324345374507343No Hit
ACTTTGCAAGGAGAACCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1488130.19089848103879828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1472770.18892808821777057Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGTC1468430.18837134962120416No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA1461430.18747338414287124No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1434180.18397773281650373Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1362060.17472612277402216No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1321990.1695859118144792No Hit
GTCGGTTTCTATCTACTTCAAATTCCAGATCGGAAGAGCACACGTCTGAAC1313590.1685083532404797Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC1309730.16801318941957039Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC1307210.16768992184737053Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1272380.16322190218875107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA1271670.1631308228330916Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1266710.1624945501513014Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1265430.1623303507495491No Hit
GCATGCTCATAAGGAAAGGTTAAAAGATCGGAAGAGCACACGTCTGAACTC1248140.16011237601806677Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1200440.1539933826871409Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC1174950.15072350553818284Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT1132600.1452908143942686Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA1098270.14088693512695866Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAAC1079760.13851245784068114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC1078930.13840598479110738No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1070210.13728737636666977Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT1054110.13522205576650403Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACT1053160.135100189023016Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCACACGT1044190.13394951040292366No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1023440.13128768416357958No Hit
ATGCTCATAAGGAAAGGTTAAAAAAAGATCGGAAGAGCACACGTCTGAACT1004550.12886446017990688Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCACACGTCTG980740.1258100947457487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAACAGATCGGAAGAGCACACGTC972770.12478769690827535No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA958290.12293019117595237Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC935650.12002591425745843Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG928820.11914975651217072Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG925520.11872642992952806Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC925400.11871103623561378No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC921280.11818251941122353Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTTGCAAGGAGAACCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT896370.11498704511618447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAAAGTATAGGCGATAGAAATTGAAACCAGATCGGAAGAGCACACGTCT895860.1149216219170488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAACCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG891690.1143866910535276Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG849010.10891166725134908Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT848260.10881545666438484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA840900.10787131010430909Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCATATTGAATTGCAAATTCGAAGAAAGATCGGAAGAGCACACGTCTGAA833040.10686302315292381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT831620.10668086444160485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAACCAAAGCTAAGACCCAGATCGGAAGAGCACACGT822280.10548272193194347No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC820100.10520306982583408No Hit
ATAAAGTATAGGCGATAGAAATTGAAAAGATCGGAAGAGCACACGTCTGAA817140.10482335870928187Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAAC804640.10321984892654448Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC800310.10266439313780426No Hit
AGTTCAACTTTAAATTTGCCCACAGAAGATCGGAAGAGCACACGTCTGAAC792190.10162275318293806Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT784900.10068758627764561Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)