FastQCFastQC Report
Tue 23 May 2023
EGAF00002207461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84794891
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC36385734.291028571520895Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA26591363.135962519251307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC24533272.893248603857513TruSeq Adapter, Index 5 (100% over 50bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC16397041.933729710201526Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTTGTAAATTTAACTGTTAATCCAAAGAGGAGATCGGAAGAGCACACG16280901.920033130297909No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT13375371.5773792314916708Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTTGTAAATTTAACTGTTAATCCAAAGAGGAGATCGGAAGAGCACACGT12083191.424990333438839No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT8926511.0527179049030206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT7717440.9101303049024498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC6853800.8082798290288504Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTTGTAAATTTAACTGTTAATCCAAGATCGGAAGAGCACACGTCTGAA6840740.8067396418965854Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT6374540.7517599144033336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCTTGTAAATTTAACTGTTAATCCAAGATCGGAAGAGCACACGTCTGA5145310.6067948126733249Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3865080.45581519763967854No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC3803180.4485152295319302No Hit
CCCCTTGTAAATTTAACTGTTAATCCAAAGAGAGATCGGAAGAGCACACGT3248250.3830714282066829No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG3243150.3824699768763191No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCT3164770.3732264954500619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACTC2810360.3314303452551169Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT2741290.32328480733585707Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTTGTAAATTTAACTGTTAATCCAAAGAGATCGGAAGAGCACACGTCT2671540.3150590759058821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTTGTAAATTTAACTGTTAATCCAAGATCGGAAGAGCACACGTCTGAAC2630820.3102568997936444Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG2604610.30716591168210833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCCTTGTAAATTTAACTGTTAATCCAAAGAGGAGATCGGAAGAGCACAC2265210.26713991530456715No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA2229420.2629191421450144Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTTGTAAATTTAACTGTTAATCCAAAGAGATCGGAAGAGCACACGTCTG2185790.2577737849795691Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2161460.25490450833883377Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTCC2069290.24403474968792638Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTTGTAAATTTAACTGTTAATCCAAAGAGAGATCGGAAGAGCACACGTC2026130.23894482039018128No Hit
CCCCTTGTAAATTTAACTGTTAATCCAAAGAAGATCGGAAGAGCACACGTC1967950.23208355795869823No Hit
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1767590.2084547758897408Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTTGTAAATTTAACTGTTAATCCAAAGAGGAGATCGGAAGAGCACACGTC1766370.20831089929698715No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1731010.20414083673979838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTCATAAGGAAAGGTTAAAAAAAGATCGGAAGAGCACACGTCTGAACT1630160.19224743151093857Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTTGTAAATTTAACTGTTAATCCAAAAGATCGGAAGAGCACACGTCTG1582590.18663742370987896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAGATCGGAAGAGCACACGTCTGAACT1542030.18185411665898596Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTG1537460.18131516909432668Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTTGTAAATTTAACTGTTAATCCAAAGATCGGAAGAGCACACGTCTGA1458460.17199857005535862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1437130.16948308831483727Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAAAGCACCCAACTTACACTTAGGAGATAGATCGGAAGAGCACACGTCTG1415000.16687326126759217Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGCGACGCTCAGACAGGCGTAGCCCCAGATCGGAAGAGCACACGTC1402560.16540619174803822No Hit
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1382210.1630062830082534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCTTGTAAATTTAACTGTTAATCCAAAGAGAGATCGGAAGAGCACACG1352080.15945300289377104No Hit
CCCTTGTAAATTTAACTGTTAATCCAAAGAAGATCGGAAGAGCACACGTCT1323020.15602590962703167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGCTCATAAGGAAAGGTTAAAAAAAGATCGGAAGAGCACACGTCTGAAC1295120.15273561705504168Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCCTTGTAAATTTAACTGTTAATCCAAAGAGGAGATCGGAAGAGCACAC1294480.1526601408096627No Hit
CCCCCTTGTAAATTTAACTGTTAATCCAAGATCGGAAGAGCACACGTCTGA1293730.1525716920846092Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC1287820.15187471613118766No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC1283590.15137586532188596No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1190480.1403952509355782No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1156610.13640090651216238Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCC1124520.13261648039620688Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1123740.13252449372215125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGCTCATAAGGAAAGGTTAAAAGATCGGAAGAGCACACGTCTGAACTC1089890.12853250793140356Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCCTTGTAAATTTAACTGTTAATCCAAAGAGATCGGAAGAGCACACGTC1088590.1283791968079775No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGAA1087310.12822824431721955Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1058440.12482355806082704No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1013230.11949186891460242Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCTCATAAGGAAAGGTTAAAAAAAAGATCGGAAGAGCACACGTCTGAAC1002440.11821938659016613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTTGTAAATTTAACTGTTAATCCAAAAGATCGGAAGAGCACACGTCTGA999450.11786677100628623Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATGCTCATAAGGAAAGGTTAAAAGATCGGAAGAGCACACGTCTGAACTCC989780.11672637211126316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA988440.11656834372250093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTCATAAGGAAAGGTTAAAAAAAGAGATCGGAAGAGCACACGTCTGAA985360.11620511429161458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATCCCCTTGTAAATTTAACTGTTAATCCAAGATCGGAAGAGCACACGTC984380.11608954129087799No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACG977940.115330061571752No Hit
ATCCCCTTGTAAATTTAACTGTTAATCCAAGATCGGAAGAGCACACGTCTG970110.11440665688219354Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT948070.11180744368195486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTTGTAAATTTAACTGTTAATCCAAAGATCGGAAGAGCACACGTCTGAA920250.10852658564063725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGATCCTTCCGCAGGTTCACCAGATCGGAAGAGCACACGTCTGAAC914120.10780366472786668Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCAGATCGGAAGAGCACACGTCTG904690.10669156942486074Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCAGATCGGAAGAGCACACGTCTGA893570.10538016966140093Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA887810.1047008834529901Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGCGACGCTCAGACAGGCGTAGATCGGAAGAGCACACGTCTGAACT882220.10404164562225807Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGT870400.102647693715415No Hit
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA854470.10076904279527879Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTC853420.1006452145802039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)