FastQCFastQC Report
Tue 23 May 2023
EGAF00002207462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80147127
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC65461598.167677676081889Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA23877112.979159814424789Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT21307852.6585918669299278Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG15200181.8965346069111124No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT7677390.957912065893516No Hit
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC6843850.853910833260436Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC5420350.6762999751694156No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT5370200.6700427327856681No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT4979920.6213472879695363Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC4837970.603636110374861No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3518560.43901261738302855TruSeq Adapter, Index 6 (100% over 50bp)
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC2401190.2995977634981226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2340620.2920404121285595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGAA2327460.2903984318739211Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA2245900.28022214695231684Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGATCGGAAGAGCACACGTCT2228500.27805113962475536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC2226100.27775169033819513No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG2222480.2773000209976335No Hit
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC2179860.2719823007504686Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC2007930.25053050248451203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1803900.22507357001081274Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT1803430.22501492785886135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTCC1776040.22159746287599302Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC1768450.22065045450724638No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTGA1711530.21354851559432692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT1709960.21335262585270212Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1602800.19998221520778903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1586280.1979210059519663No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1538780.19199440548879562No Hit
TAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAAC1529350.19081781933368616Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC1521990.18989950818823487No Hit
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1507450.1880853445938243Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG1431580.17861900402243988Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTCT1374690.1715208082256024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT1358100.16945086503225498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1320200.16472206171532514No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1313590.16389732847192387No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCT1288850.16081050540963246Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG1274070.15896639688656589No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1257040.15684155465734909Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT1187190.1481263327130865Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1161670.14494218863266303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCC1147600.14318666719020384Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG1146240.14301697926115303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA1145120.1428772362607583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1144580.14280986017128225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT1140160.1422583744018672No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA1068960.1333747122339145No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC1046140.13052744860087123No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1044520.13032532033244312Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1021080.12740069896703846Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG1008220.12579614987322005No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAAGATCGGAAGAGCACACGTCT973760.12149655720035979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT967770.12074918168931993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG909400.11346632549910367No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGATCGGAAGAGCACACGTCTGAACT902190.11256672993406239Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC900100.11230595951368287No Hit
AACCTGCATTAAAAATTTCGGTTGGAGATCGGAAGAGCACACGTCTGAACT896630.11187300575353125Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA886450.11060284169637172Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA856220.10683102839107383Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTCTGA849920.1060449740138533Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACTC818130.10207851867229127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)