FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002207578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67896499
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC34911405.141855694208916No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG15525102.286583289073565No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT13790382.0310885249031765Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC12818251.8879103030039885No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG7632401.1241227622060455No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA7485771.10252665605041Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC6884751.0140066279411548No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG6154770.9064929842700726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC6118800.9011952147930337Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC6100300.8984704793099861No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT5664770.8343243147190845Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA5527420.8140949947949452Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT4973450.732504631792576No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC4195260.6178904747356708No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT3783490.5572437542029965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA3761340.5539814357732936Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC3456360.5090630667127624No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA3181280.468548459324832No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA3145560.4632875105975641No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA3100660.4566745039387083No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT3072100.45246810148487926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA2967540.43706819110069284Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2778090.40916542692429547No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2767160.4075556237443112No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2562550.37742004930180567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2493530.36725457670505224No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2268810.33415714115097456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC2235080.32918928559188304No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC2228090.32815977742828834No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT2220540.3270477907852068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT2168320.3193566725730586No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC2087210.3074105485173838No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2051110.30209363225046404No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG2022220.2978386264069374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT1961290.28886467327277066No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1948760.28701921729425256No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1943680.28627101965890756Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1927470.2838835622437616No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC1891180.278538662207016No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1859140.2738197149163759Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1854540.2731422131205911Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC1814660.26726856711713515No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC1789280.2635305246003921Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1733540.25532097023146955No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1654950.24374599933348554Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG1574340.23187351677735252No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC1557910.2294536571024082No Hit
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACA1514310.22303211834236109No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACA1490470.21952089164420685No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1432350.21096080373746517No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1411240.20785165962680935Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA1395910.205593811250857Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGACAGCTGAACCCTCGTGGAGCCATTCAGATCGGAAGAGCACACGTC1390440.2047881732458694No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1382770.20365851264289783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG1380480.2033212345749963No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT1379400.20316216893598596No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC1357510.19993814408604485No Hit
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG1317980.1941160471322682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1316620.1939157422535144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1313190.1934105615666575Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG1295980.1908758211524279No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1258520.18535860000675441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC1250570.18418770016403938No Hit
AACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCTG1221630.17992533017055856Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA1212490.17857916355893402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1193570.17579256921627137Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACAGATCGGAAGAGC1139790.1678716895255527No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG1134720.1671249647201986No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1130100.16644451726443216No Hit
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCAGATCGGAAGAG1126780.16595553770747443No Hit
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG1126270.1658804233779418Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1102460.1623736151697601No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1074730.1582894576051705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACACTCCTTAGCGGATTCCGACTTCCAGATCGGAAGAGCACACGTCTGA1065980.15700073136318854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1014550.14942596672031647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCCAACTACGAGCTTTTTAACTGCAGCAGATCGGAAGAGCACACGTC1001040.14743617340269635No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT981220.14451702436085842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG971380.1430677596498753No Hit
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGA969140.14273784573192794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGTCTGAA962760.14179818019777427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA962330.14173484850816828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA957400.1410087433226859Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT946770.1394431250424267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCGACGTCGCCGCCGACCCCGTGCGCTAGATCGGAAGAGCACACGTCTG940780.13856089987791564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACAC936010.13785835997228665No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG925700.13633987225173422Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTTTGACAGGTGTACCGCCCCAGATCGGAAGAGCACACGTCTGAACTC923340.1359922843738968Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT899720.132513459935541Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG889990.1310803963544571Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGATCGGAAGAGCACACGTC862970.1271008097192169No Hit
ACCACCACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCT861440.1268754667306189Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC852840.12560883293849953No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC852510.12556022954880192No Hit
ACTTTGACATTCAGAGCACTGGGCAGAAATCAGATCGGAAGAGCACACGTC849470.12511248923158763No Hit
ACTCCTTAGCGGATTCCGACTTCCATGGCCAGATCGGAAGAGCACACGTCT846580.12468684136423588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA845290.12449684629541798Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGATCGGAAGAGCAC841840.12398871994857938No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT823830.12133615313508286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG813670.11983975786439298Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC805550.11864381991183374No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA803260.11830654184393219Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGACAGCTGAACCCTCGTGGAGCCAGATCGGAAGAGCACACGTCTGAA802610.11821080789452781Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC791200.11653030887498338No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG790580.11643899341555151Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT784970.11561273579069224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC783850.11544777883171856No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT763980.11252126563992644No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA759810.11190709553374763Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGACAGATCGGAAGAGCACACGT758560.11172299178489307No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC756830.11146819219647836TruSeq Adapter, Index 6 (100% over 50bp)
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTAGATCGGAAGAGCAC754970.11119424581818277No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACCAGATCGGAAGAGCACACGT750860.11058891269194895No Hit
AGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTAGATCGGAAGAGCACAC748000.11016768331456972No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAATAGATCGGAAGAGCACACGTCTG747070.11003071012542193Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC741670.10923538193037023No Hit
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG736290.1084429993953002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG729850.1074944968812015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA727850.10719993088303419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGAA725720.10688621809498602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACATTGTTCCAACATGCCAGAGGCTGTTCAGATCGGAAGAGCACACGTCTG723120.10650328229736854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACAGATCGGAAGAGCACA720540.10612329215973272No Hit
AGTTATCCAAGTAGGAGAGGAGCGAGCGACCAGATCGGAAGAGCACACGTC715440.1053721488644061No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGT712520.10494208250708184No Hit
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACA708710.10438093428057314No Hit
ACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGATCGGAAGAGCAC706520.10405838451257995No Hit
AGGACACCTGCGTTACCGTTTGACAGGTGTAGATCGGAAGAGCACACGTCT702360.10344568723639196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACACGTCTGAA701570.10332933366711589Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA694290.1022571134337869Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)