FastQCFastQC Report
Sun 14 May 2023
EGAF00002207580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77636734
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC57226697.371084157146539No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG17028952.1934140094043624No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT15368841.979583530651869Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG9214991.1869368435823175No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG9086781.170422753744381Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT6206220.7993922052414002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC5460550.7033461763087562No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT5382360.693274912878226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC5159480.6645668531084783No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT5123420.6599221445868653No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA4714250.6072190002222402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA4509700.5808719362151428Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG4180310.5384448552408194No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA3983240.5130612526796916Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA3733310.4808690174937035Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3450370.44442492905484665Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA3446510.4439277417311243No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC3216310.41427682931639037Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC3120720.40196435877892545No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3088860.39786063128312427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2813110.3623426508384549No Hit
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2768030.3565361211614079Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC2732130.35191202144077827No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2691850.34672375579323056No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT2664560.3432086671755151No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC2584490.3328952503334311Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA2323270.2992488066280583No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2320210.29885466330925253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT2299280.2961587745306236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC2284180.29421381893782395No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC2245630.28924838595090824No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT2211240.28481878178955855No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2014130.2594300270281849No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC1928880.24844940025426626No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1875070.24151840287356757Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1808090.23289104356193036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACACTCCTTAGCGGATTCCGACTTCCAGATCGGAAGAGCACACGTCTGA1733820.22332469575549121Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1732430.2231456567969487No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA1688230.21745247552530997No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1587380.20446249065551883TruSeq Adapter, Index 8 (100% over 50bp)
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC1572810.20258580171597634No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC1561600.20114189759708337No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1556830.2005274977177685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1520960.19590726214732318Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1517920.19551569492864035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1508120.19425340586841275No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC1507630.19419029141540137No Hit
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAG1472880.18971431745183923Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC1457080.1876791983547376No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGAA1447040.18638599609303502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG1442550.18580766161544096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1439890.18546504029909347Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA1430230.18422078393972627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA1419970.18289924457667164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1409660.18157126496330978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG1406270.18113461599247593No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG1404910.18095944118411783No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1395440.1797396577759183No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG1393850.17953485781614667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGACAGCTGAACCCTCGTGGAGCCATTCAGATCGGAAGAGCACACGTC1364030.1756938925328827No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT1355060.174538511627756Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG1340480.1726605346381521Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1304810.16806606006893593No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1278640.16469523305810366Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCTG1277110.16449816139870077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1245410.16041504270388293Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1227430.15809912869338372Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG1227230.1580733676921546No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC1202630.15490476454097102No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1171200.15085642319781253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG1137310.14649122153953564No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1131980.14580469085677922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1118960.14412764967676256No Hit
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC1118840.14411219307602507Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1093180.14080705661832707Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTAGATCGGAAGAGCACAC1073460.1382670218971344No Hit
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT1052060.135510594765617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAGCTTTTTAACTGCAGCAACTTTAATAGATCGGAAGAGCACACGTCTG1047830.13496574958962082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1022020.131641292381001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1020270.13141588362024606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1015970.13086202209381967Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG964100.12418090642504359Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACCAGATCGGAAGAGCACACGT933660.12026008203796928No Hit
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT929800.119762894714247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC913050.11760541086130696No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT905180.11659171546294052No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC902720.11627485514782217No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA893470.11508340884097469Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG888750.11447544921196712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACATTGTTCCAACATGCCAGAGGCTGTTCAGATCGGAAGAGCACACGTCTG870100.1120732358473503Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT863580.11123342720728051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC856600.11033436826438373No Hit
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA833070.10730358646977602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT832970.10729070596916146Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAGACAGCTGAACCCTCGTGGAGCCAGATCGGAAGAGCACACGTCTGAA832330.10720827076522822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT820060.1056278333398208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT814010.10484856305263948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT813690.10480734545067287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGACATTCAGAGCACTGGGCAGAAATCAGATCGGAAGAGCACACGTC812200.10461542599151583No Hit
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCAGATCGGAAGAGCACACGTCT805060.10369575824763572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGACATTCAGAGCACTGGGCAGAAATAGATCGGAAGAGCACACGTCT798850.10289587915947107Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCCAACTACGAGCTTTTTAACTGCAGCAGATCGGAAGAGCACACGTC798640.10286883010818049No Hit
AGACATGCATGGCTTAATCTTTGAGACAAGCAGATCGGAAGAGCACACGTC784380.1010320707205432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)