FastQCFastQC Report
Sun 14 May 2023
EGAF00002207585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78849355
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA70038378.88255458779593Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC49181576.237409297767877Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG24707183.1334663422421145No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG11973051.5184715207879633No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC9274711.1762569268955974No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC9129181.1578002128235545TruSeq Adapter, Index 3 (100% over 50bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT8292251.0516573027135097Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT6569520.8331735877864822Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT5690360.7216748951212093No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT4718670.5984411667032661Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG4299890.5453297620506851No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA4182020.5303810031166393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3845800.48774019774797145No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC3280080.41599325701522866No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT3081300.390783158594005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC3078160.39038493085961196No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT3023980.3835136000795441Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2763460.35047338053684773No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2620050.33228553359757984No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2617430.3319532544051882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC2554040.32391387348698036Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2185010.2771119687662632No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2094410.26562170356371334No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC2021440.2563673475832491Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1921970.2437521524430986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT1836920.2329657611022944No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1792220.2272967229725595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1726470.2189580371329607No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1719220.21803856226851825No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1619380.20537644220425139Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1581580.20058249049722726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1544880.19592804532135993Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1444620.18321265912701507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1411990.17907438811642784No Hit
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC1384320.17556516473723852Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC1370580.17382260133897098Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG1347190.1708561851901008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1345100.1705911227809029No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1299010.16474579912543355Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1267890.16079903253488884Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1170230.14841338905055088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1128960.14317935764978673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1121770.1422674922324983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC1093080.13862890825169083Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1074320.13624968777487145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1070510.13576648788059206Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1065630.13514758617873285No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1051440.13334795192681032No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1037560.13158763315185013Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1035090.13127437757734353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1026540.1301900313578976Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA1020470.1294202089541506Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG1006520.12765101249084407No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1001740.12704479320090825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC998630.12665037019009223No Hit
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC964180.12228127928250015Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG956370.12129078291128698Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACA934990.11857928324207599No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG916570.11624318296579597Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC893800.11335539777085052No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG883710.11207574240778508No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT880270.11163946743762204Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC877050.11123109377369035No Hit
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA820580.10406933576057789Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA803550.10190952101003237Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA798940.1013248618203662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTG793070.1005804042404659Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)