FastQCFastQC Report
Tue 4 Apr 2023
EGAF00002207586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120566126
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC66315565.500347585191548No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT28157322.335425457727654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG20975671.7397647826886302No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA20647381.71253574158964Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG16535941.3715245358385322Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC14296071.185745156977176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC9759410.8094653385479103No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT8330700.6909652218567593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT8319220.6900130472799632No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA7308480.6061802135037498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT6937780.5754336006450104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC6907820.5729486572372741No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG5907650.4899925207848181Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG5837250.4841534014288557No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC5813720.48220177531456887No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA5412290.4489063536801373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA5122490.42486975155857626Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC4734200.39266418828120925No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA4550610.3774368598357386No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG4525270.3753351086357374No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC4299850.3566383148115749No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC4259580.3532982390095208Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC4226400.3505462222448783No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC4194780.3479235950568736No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT4059370.3366924139206397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT3649560.3027019380219615No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC3386680.2808981355177656No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA3328770.2760949621952687No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC3180100.26376396965761345No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC3066460.25433843665176736No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG3031720.25145703031048705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC3024890.2508905362025151No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA2942830.24408431270322148Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT2924610.24257310880172098Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC2862690.2374373379136359No Hit
AGTGAAAAAACGATCAGAGTAGTGGTATTTCAGATCGGAAGAGCACACGTC2842500.235762738200612No Hit
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG2792230.2315932420354951No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA2662230.2208107773156782Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT2640860.21903830599981292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG2613530.2167715001475622No Hit
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG2607800.21629624227952715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA2508320.20804516850777807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2463230.20430531209072772Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA2451040.20329424866815413No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2440270.20240096293713544No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC2415800.20037137130872068No Hit
ACACACTCCTTAGCGGATTCCGACTTCCAGATCGGAAGAGCACACGTCTGA2376990.19715239087967376Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC2317800.19224305175070483No Hit
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA2238670.1856798484177886Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2208370.18316670471770818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG2207890.18312689254028117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG2194360.18200468678905715No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2191950.181804796481559Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2153850.1786447048982896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG2008720.1666073271691586No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1981200.16432476233000967No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT1956680.1622910235997796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCTG1955990.16223379359472825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGACAGCTGAACCCTCGTGGAGCCATTCAGATCGGAAGAGCACACGTC1925240.15968332597831003No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1892390.1569586800856486Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG1887860.15658295266118114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1805780.14977507032116136Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG1799540.14925751201461016Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG1759080.1459016772256579Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1757970.14580961156535793Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC1753080.14540402500782018No Hit
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT1731640.14362574774941345Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1726790.14322347887332798No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1716440.14236502879755794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAG1709820.14181595251721035Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1674870.13891712834830572No Hit
AGGGCGCCCTGCCCTTCACAAAGAAAAGAGAACTAGATCGGAAGAGCACAC1621230.13446811752083665No Hit
ACGAGCTTTTTAACTGCAGCAACTTTAATAGATCGGAAGAGCACACGTCTG1607380.13331937031799462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT1606890.13327872872020455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTGA1561170.12948661882028126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1547500.12835280118397435Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT1536650.12745288009005118No Hit
ACTTCCTCTAGATAGTCAAGTTCGACCGTCTTAGATCGGAAGAGCACACGT1520100.12608018938918217No Hit
CAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGT1519660.1260436948932074No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG1496220.12409953356218811Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA1488920.12349405669715224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT1463790.12140972332477533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1457390.1208788942924151No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTAGATCGGAAGAGCACACGTCTG1446600.1199839497206703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAGACAGCTGAACCCTCGTGGAGCCAGATCGGAAGAGCACACGTCTGAA1412330.11714152613645394Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT1407370.11673013363637477No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1394070.1156270045535012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCAGGTCGGACGACCGATTTGCACGTAGATCGGAAGAGCACACGTCT1389770.11527035379738418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA1369820.11361566017307383Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTTGACATTCAGAGCACTGGGCAGAAATCAGATCGGAAGAGCACACGTC1364710.11319182636754871No Hit
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT1360260.11282273430598576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACATTGTTCCAACATGCCAGAGGCTGTTCAGATCGGAAGAGCACACGTCTG1338430.11101210965341957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACG1323150.10974475533865956No Hit
TGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAGATCGGAAGAGCACAC1320210.10950090575191906No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1300990.1079067598141123Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC1261580.10463801416328165No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC1251690.1038177174242125No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1233950.10234632570013903Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGTCTGAA1219540.10115113095696547Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1215330.10080194498411602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTGA1211060.10044778248908819Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAAC1210420.10039469958585216Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)