FastQCFastQC Report
Sat 13 May 2023
EGAF00002207591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76065783
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA1017062713.370830613812256Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC73368389.645385494815716Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG36793444.83705531566013No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT10138771.33289497591841Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT9629571.2659529186730385No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT8603561.1310683543479727Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT8042311.0572835357522055Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG5489640.7216963769373149No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC5058700.6650427827713283Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4973220.6538051412683151No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA4747280.624101904005905Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC4321780.5681634802865304No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT3732490.49069237872697635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG3349330.44032018969685754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2886860.3795214991739453No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2807400.3690752779078078No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2795960.3675713165274326No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2689560.35358342396869824Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC2577250.3388185723402071Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA2550600.3353150259427422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA2380000.31288707039274155Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2316380.3045232571917389TruSeq Adapter, Index 9 (100% over 50bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA2209940.2905301060267795Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC2178080.2863416261684968Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA2095510.2754865482683587Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACG1920820.25252090023184276No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1914280.25166111811404085Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA1835620.24132006897240513Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA1829200.24047606267327848Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1810040.23795719029146128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1806960.2375522776121295No Hit
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA1791410.2355079944421265Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1702850.22386544025978145No Hit
GGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAA1607160.21128553951781448Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1566930.20599669630693213Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1518840.19967453697281998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1498140.1969532082513369Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC1466770.19282914631931153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1465630.19267927604189652Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1393860.18324402182253222Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1361530.17899375334110476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC1257350.16529771342786284Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1249750.16429857824509608No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT1236020.16249356165833462Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC1235960.16248567374899697No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1235820.16246726862720917Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1209870.15905574783868326No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1209590.15901893759510763Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1201880.15800534124522192Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC1192400.15675905156987605Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1170320.1538563009336274No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1139080.14974932947183361No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT1131550.14875939684996078Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1089240.14319710611537384No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT1081010.14211514788456198No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTG1076610.14153670119980227Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG1036150.13621762100312568Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTC1019170.1339853426605758No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1013880.1332898919873079Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1010670.13286788883774456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG980230.12886608950045253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC964760.1268323235428997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAAC917630.12063637075818966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG901990.11858025572418021Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG898870.11817008443862334No Hit
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC890090.11701582037221651Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG878260.11546058758114669Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT873280.11480589110612323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA853650.11222523010116126Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA837920.11015728320314536Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT828320.10889521770912423No Hit
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT820770.10790265578413884Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTG794620.1044648419644875Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)