FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002207593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56750162
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA1013162117.853025688279093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC581224810.241817459481437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG46441258.183456815506537No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT22667913.9943339721215247No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT7845861.3825264498804426Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6757421.1907314026698286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA5149360.9073736212418214Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG4585260.8079730239360374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4289570.7558692079152126No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC3419360.602528676482016No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3383170.596151602175162No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2925860.5155685722976439No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2716640.47870171718628746No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2711830.4778541425132848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC2541400.4478225101806758No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2399150.4227565024395878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA2193020.38643413916598157Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT2010680.3543038344101996Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1931440.3403408786744961TruSeq Adapter, Index 1 (100% over 50bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1684290.2967903422020188Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1617240.28497539795569216No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1612620.28416130336332784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1603760.2826000743398759Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1475610.26001864100405564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC1414190.2491957644103289Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1397080.2461807950433692Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1358680.2394142945354059Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1278360.22526103097291597No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1234370.21750951125038198Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1126130.19843643794356042Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1120450.19743555974342414No Hit
ACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTCTGA1067010.18801884653650858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG1065020.18766818674455943No Hit
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG871900.1536383279399273Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA840360.1480806345539595Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC823480.14510619370566732No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG818540.14423571160906992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC817510.14405421432981988Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT757930.13355556588543308Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC740770.13053178597093698Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTATAGGTAGAGGCGACAAACCTACAGATCGGAAGAGCACACGTCTGAAC740200.13043134572902187Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT723950.12756791777968846Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT702020.12370361163021878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG652870.11504284340192722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT636200.11210540685328793No Hit
AACACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACG635770.11202963614447478No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC631930.11135298609367845No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG629760.11097060833059824No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA627900.11064285596224378Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA619800.10921554726134525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT605850.10675740449868673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA602380.10614595249965983Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC602110.1060983755429632Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA587350.10349750191021481Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA577850.10182349787829681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)