FastQCFastQC Report
Tue 23 May 2023
EGAF00002207594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109315207
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA86904047.949858247992888Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC69262736.336056245129737Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC12651121.1573065035681631TruSeq Adapter, Index 2 (100% over 50bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG11714071.0715864993971058No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGTCTGAA9551170.87372747690996Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG8689010.7948583036576055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC8506420.7781552295830167No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA7224220.6608613932368989Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAA6814440.6233753003824984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC6622670.6058324529358482No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT6546890.5989002060802026Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACTCCA6131310.5608835374569615Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG5495730.5027415810501096No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC5222260.4777249335492728No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA5059480.4628340501610174Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA5003730.4577341192794887Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA4220900.3861219418447426Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT3807470.34830195217029597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA3718210.340136574045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA3641040.33307717196199427Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA3617140.3308908338800474Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT3417690.31264543093258745No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA3323710.3040482739057522Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAAC3294980.3014200942783743Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT2914340.266599687269494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG2887870.26417824923480226No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT2860280.26165435518957575Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA2841960.259978467588686Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCTGAACTC2835760.2594113003875115Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2780480.2543543644389751Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGT2755690.25208661042008546No Hit
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC2703210.24728581449788592Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCC2677480.2449320706130118Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT2673220.2445423718586564No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2671590.244393261771896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT2548180.23310389011109864Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2544880.23280201079434445Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC2483240.22716327107170003No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2452360.22433841249552772No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2400000.21954859400302834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG2370110.21681429922188228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG2325520.21273526930246767Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCTGAA2305440.210898379399309Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2284700.2090011136327995Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG2278630.2084458386471335No Hit
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG2259910.20673335961390987Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC2174000.1988744347344098Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT2173570.19883509894465093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA2126190.19450084378470783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGAAC2101040.19220015747671776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC2096150.19175282721643658Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC2077240.1900229672528544No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2073190.1896524790004743No Hit
AGGAAGAGCCGACATCGAAGGATCAAAAAGCAGATCGGAAGAGCACACGTC2034710.18613238320995906No Hit
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA2024780.18522400090227154Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA1998580.18282726208440514Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTGAA1995550.1825500819844763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAAC1979400.18107270290399763Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1976850.18083943252286938Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1971650.1803637439025295No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTGA1967250.17996123814685727Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1938760.17735501337887968Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAAGATCGGAAGAGCACACG1881080.17207852883634023No Hit
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC1859550.17010899499097137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT1840040.16832424787888844No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC1802440.16488465323950766No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1797900.1644693404825186Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1691120.1547012576210005Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCTCCCCGGACCCCGTCCCGGCCAGATCGGAAGAGCACACGTCTGAACT1650060.15094514709193205Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1646230.15059478412733557Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC1637720.14981630140443314No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1583790.14488286154002344Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTACCGCAAGGGAAAGATGAAAAATTAGATCGGAAGAGCACACGTCTGAA1554400.14219430604929467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC1536170.14052665152068Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1524410.13945086341006518No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1499390.1371620693175836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA1487100.13603779755912643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGAGCTTTAATTTATTAATGCAAACAAGATCGGAAGAGCACACGTCTGA1483210.13568194587967985Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1465030.1340188652801069No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC1437970.13154345488272276Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC1382860.12650207029292823No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1342760.12283377920146096No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1303180.11921305697202768No Hit
AGAGTCTCGTTCGTTATCGGAATTAACCAGACAGATCGGAAGAGCACACGT1266930.11589695841677362No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1253460.11466474193293162No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1232150.1127153333753464No Hit
ATGTCTCTTCACCGTGCCAGACTAGAGTCAGATCGGAAGAGCACACGTCTG1229640.11248572213745156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGGCTTTAACATATCTGAACACACAATAGATCGGAAGAGCACACGTCTG1207520.11046221592939032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAAGTATAGGCGATAGAAATTGAAAAGATCGGAAGAGCACACGTCTGAA1182010.10812859733229979Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGCTACTGGCAGGATCAACCAGGTAGGTAGGAGATCGGAAGAGCACACGT1180330.10797491331649767No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1178650.10782122930069556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAACAGATCGGAAGAGCACACGTC1169500.10698420028605901No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT1165470.1066155416052956No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1152810.10545742277192961Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGATTACCATGGCAACAACACATCATCAGATCGGAAGAGCACACGTCTGA1147850.10500368901099004Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1133810.10371932973607231No Hit
ATCCTGAGGGAAACTTCGGAGGGAACAGATCGGAAGAGCACACGTCTGAAC1128480.10323174890022391Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAA1126030.10300762637717915Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACAGATCGGAAGAGCACA1118870.10235263973840346No Hit
ACAATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGT1106870.10125489676838832No Hit
TTTATAGGTAGAGGCGACAAACCTACAGATCGGAAGAGCACACGTCTGAAC1105870.10116341818755371Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA1097880.10043250432668531Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)