FastQCFastQC Report
Fri 24 Mar 2023
EGAF00002207596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142957050
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA1561835610.925208655326896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC57272174.006250128972303Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC22988271.6080543072202456No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA14125700.9881079666934929Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG12279470.8589621847960629No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG9991070.6988861339821995No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT9603850.6717996768959628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT9224170.6452406509507576Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC8917200.6237677680114412No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT7691480.5380273305863544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC5946720.4159794847473419No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5774660.40394370197202584No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG5727180.40062242470728093Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT5501540.3848386630809743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4883520.34160749679711494No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT4781870.33449696954434915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT4746920.3320521793084007Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC4669830.3266596505733715No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC4457290.31179224809129735No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG4280150.29940111383104223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG4058450.28389295945880244No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA3632270.2540812083069705Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTCC3615260.25289134044106254Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACG3578120.25029335734054386No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT3504330.24513166716856566Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC3364760.23536859497310553No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT3294790.23047411792562872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3195840.22355245858808642No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT3167170.2215469611327318Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG3131460.21904900807620192No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA3089560.21611805783625224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT3077810.2152961326496315No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCCA2939540.20562399685779748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA2847830.19920878333737302Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2841840.1987897763698957Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2601750.18199522164174486No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG2479820.17346608649241152Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA2384420.16679275348784828Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAAC2359880.1650761539917059Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACG2301660.1610036021308498No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2259130.1580285827106813No Hit
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA2206110.15431977646432968Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA2205960.15430928380237283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2189950.15318936701617722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG2098400.14678534566850673Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2057730.1439404352566033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC1975710.13820304769859199No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT1957010.13689496250797004Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC1943930.1359800023853318No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT1923170.13452781797050234Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT1822040.127453665279187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC1816650.12707662895953714Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1790740.1252641964841888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTG1790060.12521662974998435Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACT1771200.12389735238660841Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1741290.12180511559241045No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1735710.12141478856761524Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1704730.11924770411812499No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT1690410.11824600465664338No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG1659930.11611389574700934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC1646940.1152052312215452No Hit
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC1646590.11518074834364588Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1636630.11448403558971033No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1610800.11267719920073896No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACTC1607550.11244985819167366Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT1605040.1122742809815955Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACACGT1593920.11149642497519359No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC1584920.11086686525778196No Hit
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1576380.11026948303703805No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1558140.1089935753430838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACTCC1548770.1083381337261786Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTC1548460.10831644889146776No Hit
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1541440.10782539231188669Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1539130.10766380531775104Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT1532680.10721262085360603No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCCA1531330.10711818689599428Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1516990.10611508841291842No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1480320.1035499823198646No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1453500.10167389436197795No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTC1445060.10108350724920526No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC1429730.10001115719721412Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)