FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002207597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66772176
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA62791159.403789686290889Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG43695546.543974244601523No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC38299755.735884659502485Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT28978434.3398960069835075Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC12713081.9039487345747126No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT11057361.6559831747882532No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT9523841.426318651050102Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG7826461.1721139655535564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG6909551.0347947923698038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT6873181.0293479128192558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC5854190.8767409347270636No Hit
AGGCATGCCCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA5640120.8446811737871176Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGCCCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT5017140.7513818330557327Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC4280920.641123332568943No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG4077860.6107124620290943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT3766360.5640612940336106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT3637760.5448017749189422No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT3618030.5418469513409299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCATGCCCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA3352160.5020294680826337Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG3149200.47163357384069676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA3084880.4620008190237802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC3076150.46069338821607375No Hit
GGCATGCCCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC2874050.4304262901361789Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA2729160.40872713209166645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2460530.36849630301100267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2299910.3444413733049526Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATGCCCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAA2238300.33521447616144784Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC2017750.3021842511168125Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1991540.2982589634341106Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG1877040.28111110232501635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC1854290.2777039945500653Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC1847330.27666164421539896No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1813710.27162661285742734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1734580.2597758683197624TruSeq Adapter, Index 5 (100% over 50bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1647240.24669556972353274No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA1535640.22998202125388273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT1497180.22422213707697652No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1435430.21497427311639505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAAC1426790.21368032097680928Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1358290.20342155690717645No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1350040.2021860123294469No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1331680.19943636403282708No Hit
GCATGCCCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1289710.1931508117992141Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1229070.18406918474545444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAA1128920.16907042238671388Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTGA1099010.16459101168127274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1092780.16365798832136308No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1039180.15563069264059928Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC1028680.15405818135985266Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGATCGGAAGAGCACACGTCTGAAC1025700.15361188768207884Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC984180.14739372878906926No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGTC942480.14114861255981834No Hit
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA877730.1314514596618807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA858390.1285550436457245Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA828110.12402022063800947No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG826460.12377311172246355No Hit
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT808400.12106839231957935Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT774590.11600490599557516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGCCCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC762980.11426615780800674Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTGAA762000.11411939008847038Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC754740.11303211086006844No Hit
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC741300.11101929642071272Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA728180.10905440613467501Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG726420.10879082329142606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG713520.10685888086079448No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT678150.10156176428936507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)