FastQCFastQC Report
Tue 23 May 2023
EGAF00002207598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80871748
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA973915012.042709896662553Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC58422137.224046894596616Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG22828932.822856011471398No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC13492181.6683428185576006TruSeq Adapter, Index 6 (100% over 50bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT7922890.979685761212927No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA7113230.8795692161865971Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4975840.6152754358666762No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT4648600.5748113667581416Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4584810.5669235689081433No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG4021170.49722803073330385No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC4012220.4961213401743214No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA3949410.48835472184921735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG3927000.48558366761158667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA2910590.3599019524098824Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT2737610.33851252974029944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAAGTATAGGCGATAGAAATTAAAAAGATCGGAAGAGCACACGTCTGAA2595650.3209588099913458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC2562290.31683376004188757No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA2537430.3137597569920215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT2492490.30820281020758944Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC2480160.30667817394029867Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA2273990.28118472225924934Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA2239820.27695951372288874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2099980.25966793743595107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2066440.25552063002273673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT2033610.2514611159387825Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG1957130.24200416689398133Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1729510.21385836744866699Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG1638070.2025515758605836No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT1591360.19677576401588354Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCT1563930.19338397384461134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAAGTATAGGCGATAGAAATTAAAACAGATCGGAAGAGCACACGTCTGA1480100.183018178363104Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1451110.17943349017261256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1406870.17396310019167632Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1399780.17308640342484002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1388420.17168171015667918No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1379210.17054286992782697No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA1315050.16260932062455233Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1311340.16215056956602447Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATAAAGTATAGGCGATAGAAATTAAAAAGATCGGAAGAGCACACGTCTGA1298120.16051588250571758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1278330.15806879801831414Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGTCTGAA1277400.1579538011222411Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA1263600.1562473955676091Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC1241200.15347757785574265No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC1236780.15293103346795472No Hit
TAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTG1205320.14904092341369943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC1161680.14364472497861677No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC1156320.14298194716899157No Hit
ATAAAGTATAGGCGATAGAAATTAAAACCAGATCGGAAGAGCACACGTCTG1145570.14165268197244854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1143430.14138806545890414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1131470.13990918064488975No Hit
AATAAAGTATAGGCGATAGAAATTAAAACAGATCGGAAGAGCACACGTCTG1074310.13284119937657338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACT1073920.13279297487176858Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1061820.131296778697055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG1035110.1279940183808071Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGAACTCC1030250.12739306685939322Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1021570.12631976249604496No Hit
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC955360.11813272541110402Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAATAAAGTATAGGCGATAGAAATTAAAACAGATCGGAAGAGCACACGTC922130.11402375029658071No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT920500.11382219659701186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT885640.1095116677829197Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT867680.10729086751036963Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG867320.1072463525828575No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACG819300.10130855586304378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)