FastQCFastQC Report
Tue 23 May 2023
EGAF00002207599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112816463
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG87253237.734086646556186No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA77593736.877872957247383Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC47351514.197216322940385Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT25470372.257682019334359No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT24406212.1633553606444833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC14241211.262334381108899No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT11033940.9780434261620132Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT9974580.8841422372903146Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC9845020.8726580977813494No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC8634970.7653998158052517No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG8391840.7438488831191242No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG7164040.6350172492112255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC6740190.5974473778707279No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT6607810.5857132748435838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG5847760.5183427883215945No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC5593550.4958097294718414No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG5158230.45722316254499135Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT4560220.40421582796829925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC4308910.3819398238003615No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4298500.38101708613218976No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT4001070.3546530261279331No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT3962470.35123153967342513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3814380.3381049093872053Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT3692850.3273325454282324Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA3445990.305450987237563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC3281120.29083698537863223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG3218910.28532271925596536Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC3026710.2682861986197883No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT2913080.2582140870698987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC2746830.2434777626382419Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2498750.22148806420211917No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC2498330.22145083559302867No Hit
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC2437090.21602254982945177Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT2436920.21600748110672466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACG2398040.21256117557948967No Hit
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA2359710.20916362180225415Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC2260580.20037678366144132Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2147480.19035165107064206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA1987240.1761480503071613Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1981830.17566851036625744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG1922730.17042991322995119No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1863700.1651975208618267No Hit
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1747060.15485860428012177Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG1712120.15176153856197389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1625460.1440800355529671Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC1606670.14241449849389445No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG1574000.13951864454392618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC1546730.13710144413940722No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1541550.1366422912939577Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1521470.13486240922124992No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA1512400.134058448543986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAGATCGGAAGAGCACACG1442050.12782265652132704No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1441390.12776415442132769No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1388960.12311678305319676Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1380270.1223465054032052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1340010.1187778773032443Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG1278830.11335490991239462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1270300.1125988145896756Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAAC1224170.10850987235790223Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1177500.10437306477158392Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACACG1155860.10245490500796857No Hit
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA1148900.10183797377161168Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)