FastQCFastQC Report
Tue 28 Mar 2023
EGAF00002207600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133976712
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA95518977.129520390080928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC79218575.9128611844124075Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC29550402.205637051310828No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG14255111.0639990926184246No Hit
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG11325420.8453275073656085No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT11318520.8448124924875005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG10074350.7519478459808746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG9765820.7289192169457033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA7906700.5901548024256633Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT7643970.570544677943731Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG6342620.47341212553417494No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA5639730.42094853021919215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA5451110.40686996408749004Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5031620.37555929869364163No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA4724630.35264561500807695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT4531700.3382453511771509Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT4387890.32751139615965497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTC4366750.3259335099968717No Hit
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA4286470.3199414238498404Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA4012960.29952668192066095Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC3822100.28528092255316734No Hit
ATAGTACCGCAAGGGAAAGATGAAAAATTAGATCGGAAGAGCACACGTCTG3820110.28513238927672746Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA3600000.26870341466508Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG3522570.2629240520546586Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA3481140.25983172359088796Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA3355110.25042486488248794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA3287550.24538219746727327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3244210.24214730691405534No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC2977030.22220503515566198No Hit
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC2806960.20951103800785917Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC2795500.20865566547117534No Hit
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2783240.20774058106456592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT2767910.2065963523571171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG2766700.20650603815385468No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC2669530.19925328515301974No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2667420.19909579509609102Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA2579210.1925118150384225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT2517410.18789907308667197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAGATCGGAAGAGCACAC2482940.1853262378912538No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT2438080.18197789478517729Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2272300.16960410253985037No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2203170.16444425057990675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA2168580.16186245860399978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGAAC2159500.16118472888034452Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT2154780.16083242884778365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGA2081430.15535759677398264Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAAC2061250.15385136485511006Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA1986070.14823994187885428No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT1981030.14786375709832317No Hit
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG1978000.14763759839098006Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC1976450.14752190664299927Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTAACACAAAGCACCCAACTTACACTTAGATCGGAAGAGCACACGTCTGA1963250.1465366607892273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC1957060.14607464019567817Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC1899450.14177463916266284Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA1886270.140790886105639No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC1881120.14040649094299312No Hit
CGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTG1866170.139290625373759Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1864460.13916299125179307No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAAGATCGGAAGAGCACACGTCTG1858700.13873306578832895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGTCTGAA1839150.13727385696702274Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCTGA1830430.13662299758483398Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACACGTCTGAA1812130.1352570885602865Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1808960.13502048027570643Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC1703440.1271444846325233No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1665770.12433280195740287No Hit
ATAGCAAGGACTAACCCCTATACCTTCTAGATCGGAAGAGCACACGTCTGA1654590.12349832857519298Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGTATAGGCGATAGAAATTGAAACAGATCGGAAGAGCACACGTCTGAAC1648740.12306168552636222Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCT1630780.12172115404653311Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAAC1601720.11955212037148666Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1594770.11903337350150822No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1583340.11818024015994659Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGT1579470.11789138398918164No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC1574820.11754430874523925No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC1559650.11641202241177556No Hit
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA1559580.11640679762315709Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGAGTTCTTGGGTGGGTGTGGGTATAGATCGGAAGAGCACACGTCTGA1553760.11597239376944853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA1519000.11337791302118236Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1433950.10702979484971985Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAGATCGGAAGAGCACACGTCTGA1406050.10494734338606548Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAACACAAAGCACCCAACTTACACTTAGATCGGAAGAGCACACGTCTG1401110.10457862258927506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT1384590.10334557247531197No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA1370320.10228046199551456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1360570.10155272358079664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC1359250.10145419899541945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1356980.10128476656450563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG1346310.10048835949937329No Hit
GGGAGAGTTCTTGGGTGGGTGTGGGTAGATCGGAAGAGCACACGTCTGAAC1342380.10019502493836391Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)