FastQCFastQC Report
Tue 23 May 2023
EGAF00002207601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102197760
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA56888075.566469362929285Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG54945575.37639670380251No Hit
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC42166704.125990628366023Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG18810251.8405736094411465No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT17144071.6775387249192155No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC15475201.5142406252348388No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT12521041.2251775381378223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT9441430.9238392309185642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT9202710.9004805976177952Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT6835670.6688669105859072Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG6026370.5896773079957918Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC5764270.5640309533203076No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT5391460.5275516801933819Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT4733590.4631794278074196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC4656240.45561076876831746No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG4618630.4519306489692142Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT4571970.4473649911700609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG4551990.4454099581047569Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT4415310.43203588806643123No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC3724750.3644649354349841No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC3554140.3477708317677413No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA2995520.2931101425314997Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG2945280.28819418351243703No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC2919430.2856647738658851No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG2835790.2774806414543724Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2791600.27315667192705595No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2709540.2651271417299166No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2700750.2642670446005862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2670910.26134721543799005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT2644860.25879823589088446No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC2556390.2501414903810025No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC2443390.23908449656822225No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2425480.23733201197364795Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2424950.23728015173718092Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACAC2350840.23002852508704694No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA2290260.22410080220936351No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2283040.22339432879937876No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT2282880.2233786728789359Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG2212080.21645092808296384Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA2125310.2079605267277874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2086950.20420701980160819No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG1923010.1881655723178277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1903440.1862506575486586No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1842510.1802886873450064Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT1826010.17867417054933493No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1771260.17331691027278875No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1738610.17012212400741464Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1696620.16601342338618774Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1624010.15890857099020564No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1619700.1584868396332757Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC1510670.1478183083464843No Hit
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC1502190.14698854456301194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1342900.13140209726710253TruSeq Adapter, Index 9 (100% over 50bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1332050.1303404301620701No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1319360.1290987199719446No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1307880.12797540768016835Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1304850.1276789236867814Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1295660.1267796867563438No Hit
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC1258410.12313479277823702Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1153010.11282145518649334Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA1135990.11115605664938254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA1135920.11114920718418876Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC1121870.10977442167029883No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1101420.10777339933869391Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1091550.10680762474637409Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGAA1078410.105521882280003Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC1061650.1038819246136119Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)