FastQCFastQC Report
Sat 13 May 2023
EGAF00002207602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72866498
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA817075311.213319185450631Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC741249710.172709274432263Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG16216422.2254973746645543No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC14201311.948949159049746No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT10937041.500969622555485Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT9255321.270174943771828No Hit
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA5696540.781777655898874Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT5523240.7579944352478694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT4850740.6657023643430757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG4177190.5732661942941185No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG3879680.5324367310749585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC3867150.5307171479546059No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG3591700.49291513913568347Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT3269790.4487370862807212No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3156550.43319633667587537No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC2670130.3664413788624781TruSeq Adapter, Index 10 (100% over 50bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA2547440.3496037369601597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2201020.3020619983685781Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA1968460.270146096495539Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT1825720.25055684712609627Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA1796090.24649050651507914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGAA1739640.23874346205028268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1714560.23530155106397457Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC1709540.23461261991759233No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1680850.23067528234992163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC1549110.212595643062193No Hit
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA1431510.19645653891586776Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC1383050.18980602031951638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACG1379020.1892529540804884No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1370010.1880164461862844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1351750.185510493450639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCT1347020.18486136111550194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT1341460.18409832183783553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT1266450.17380415345334696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1242650.17053790618563827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1223040.16784668312178253Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1189100.16318884983329376Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAAC1180930.16206762125442065Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA1135460.1558274421257352Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAAGGAAAGGTTAAAAAAAGTAAAAGGAAAGATCGGAAGAGCACACGTCT1117590.15337501192935057Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC1113080.1527560717958478No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1078630.14802824749447957Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC1066020.1462976853917146No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1043740.14324003878984276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT1032150.14164945871283674No Hit
GTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGAA1029620.14130224839404248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC1002710.1376091931850492No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTCT935290.12835665575694336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACTC934880.12830038847207945Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA932500.12797376374530858Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG912560.12523725237900138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA891750.12238134457895862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC888020.12186944952397738Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG887920.12185572579596182Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG858530.11782232213218206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG823430.11300529359871253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA798590.10959631955964179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT786210.10789732203131266Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC785570.10780949017201293Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC779880.10702861004792628No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA761400.10449246511064658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC760050.10430719478243622No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC738970.1014142329167514No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTCT734440.1007925480376455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)