FastQCFastQC Report
Tue 23 May 2023
EGAF00002207603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100635175
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC39131253.8884266857984793Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA25643672.548181587600956Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG16808191.6702102420947744No Hit
GCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACT14245231.415531895284129Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT12693661.2613541935014274No Hit
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT11907891.1832731448025007Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTC11475491.1403060609771882No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG10366971.0301537211019904Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG10150791.0086721665660143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAA9094300.9036899871242834Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT8051670.8000850597219114No Hit
TAACCCAAGTCAATAGAAGCCGGCGTAGATCGGAAGAGCACACGTCTGAAC7951510.7901322773076114Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAAC7527410.7479899548045701Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA7327000.7280754467809094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCCAAGTCAATAGAAGCCGGCGTAGATCGGAAGAGCACACGTCTGAACT7047960.7003475673391536Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA6656530.6614516246431728Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT5830100.579330239153457Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA5349590.5315825207239914Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGA5224130.5191157068092742Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCAGATCGGAAGAGCACACGTC5038080.5006281352419767No Hit
AATAGGTTTGGTCCTAGCCTTTCTATTAGATCGGAAGAGCACACGTCTGAA4694200.46645718060310426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGATCGGAAGAGCACACGTCTG4348550.43211034312803653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC4033170.4007714002584086No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA3998440.3973203206532904Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGA3992740.39675391829944157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGT3959120.3934131381000729No Hit
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA3906310.3881654699760794Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT3892120.3867554262214976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACT3764390.3740630450535809Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACT3621250.35983939015359195Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG3611970.3589172473739923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAAGATCGGAAGAGCACACGTCT3469680.34477805598291056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAA3401150.33796830978830217Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAA3398340.337689083364738Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG3332370.3311337213851916Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACT3304480.3283623246046921Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTC3238280.3217841077933237Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC3222210.3201872506308058No Hit
AGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTGAA3218230.3197917626714516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT3082470.30630144976644597No Hit
ACGATTAAAGTCCTACGTGATCTGAGAGATCGGAAGAGCACACGTCTGAAC3038710.30195306959023027Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC3006430.29874544362843314No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCAGATCGGAAGAGCACACGTCTGA2913340.2894951988705738Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGATCGGAAGAGCACACGTCTGAAC2877910.28597456108164965Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2859820.28417697887443427Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA2789780.27721718574047294Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2772700.2755199660556063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACG2757430.2740026039602952No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2734550.27172904503817874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2668350.26515082822681035Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2655060.2638302164228363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC2602080.25856565559706135No Hit
TAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAAC2528810.2512849011292523Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAAC2505270.24894575877669017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA2467530.2451955789812061Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT2440850.24254441848985706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTAGATCGGAAGAGCACACGTCT2402990.238782314434292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAAC2392210.23771111840367945Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGTC2331530.23168141755603844No Hit
TTTATAGGTAGAGGCGACAAACCTACAGATCGGAAGAGCACACGTCTGAAC2302150.22876196121286618Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2294040.22795607996905654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAAC2280550.2266155943982807Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAA2278670.22642878099034458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAAGATCGGAAGAGCACACGTCTG2265290.2250992259913097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGTC2197710.21838388018900948No Hit
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG2141380.2127864337693058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAACTC2117220.21038568273965838Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2050610.20376672470634646No Hit
AGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGTC1954050.1941716700944774No Hit
AACCCAAGTCAATAGAAGCCGGCGTAAAGAGATCGGAAGAGCACACGTCTG1932590.19203921491665316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCCAAGTCAATAGAAGCCGGCGTAAAGAGATCGGAAGAGCACACGTCT1891680.1879740359173619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTCT1874000.1862171949320901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC1851690.18400027624535853No Hit
AGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGAAC1833570.18219971297312296Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAAC1830750.18191949286121875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCATGCTCATAAGGAAAGGTTAAAAAGATCGGAAGAGCACACGTCTGAAC1781090.17698483656435238Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT1719820.17089650810464632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGCCTTTCTATTAGCTCTTAGTAAGATAGATCGGAAGAGCACACGTCTG1676810.16662265455393702Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCTG1641580.16312189053181456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCTGGTGATAGCTGGTTGTCCAAGATAGATCGGAAGAGCACACGTCTGA1585060.15750556403364926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGCGGTGCTTCATATCCCTCTAGAGAGATCGGAAGAGCACACGTCTGAAC1552060.15422639251136594Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG1549600.15398194517970482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCCTAAACCTCAACAGTTAAATCAACAGATCGGAAGAGCACACGTCTG1519240.15096510737920413Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATAGACTACGAAAGTGGCTTTAACATAAGATCGGAAGAGCACACGTCTG1510760.15012245966681134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACTCCA1502880.14933943325482366Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGAGCTTTAATTTATTAATGCAAACAGATCGGAAGAGCACACGTCTGAA1499160.14896978119231175Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGATCGGAAGAGCACACGTCT1495640.14862000289660152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAACTC1464020.14547796036525001Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1422200.14132235572701096No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA1418090.14091394981923566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGGTTTGGTCCTAGCCTTTCTATTAGCAGATCGGAAGAGCACACGTCTG1369860.13612139095500156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGTC1364300.13556890023791382No Hit
ATAGACTACGAAAGTGGCTTTAACAGATCGGAAGAGCACACGTCTGAACTC1362610.1354009669084393Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1345290.1336798987034106TruSeq Adapter, Index 3 (100% over 50bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAGATCGGAAGAGCACACGTCTGAA1335670.132723970520248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA1323840.13154843721392645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGGCTTTAACATATCTGAACACACAGATCGGAAGAGCACACGTCTGAAC1305890.12976476664347233Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTAGCATCACCAGTATTAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1299910.12917054101610098Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCAACTTTAAATTTGCCCACAGAACAGATCGGAAGAGCACACGTCTGA1292180.1284024199292146Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGACCTGGCGGTGCTTCATATCCCTCTAAGATCGGAAGAGCACACGTC1245440.12375792062765331No Hit
CATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGAACT1231820.12240451710845636Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1227040.12192953408189532No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1211270.12036248756957993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGAGATCGGAAGAGCACACG1202200.11946121224512204No Hit
TTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAACT1194780.11872389549677834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACCCAAGTCAATAGAAGCCGGCGTAAAGAAGATCGGAAGAGCACACGTCT1191140.11836219294098708Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTG1178630.11711908882753967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCAGCAAACCCTGATGAAGGCTACAAAGTAGATCGGAAGAGCACACGT1165440.11580841390696642No Hit
ACCGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTC1157460.1150154506115779No Hit
CCCTTGTAAATTTAACTGTTAGTCCAAGATCGGAAGAGCACACGTCTGAAC1137740.11305589720492859Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1113360.11063328503179927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACCTGGCGGTGCTTCATATCCCTCTAGATCGGAAGAGCACACGTCTGA1084820.10779729850919423Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAAC1083210.10763731468644039Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAACT1075220.1068433577027118Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT1069630.10628788592060381Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCT1060920.10542238337638901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACTC1054420.10477648595533322Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTATAGGTAGAGGCGACAAACCTAAGATCGGAAGAGCACACGTCTGAACT1048700.10420809622480409Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTAGGAAAAAACCTTGTAGAGAGAGTAGATCGGAAGAGCACACGTCTGAA1033210.10266887298601111Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAAAGATCGGAAGAGCACACGTCTGAA1021050.10146054796446669Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)