FastQCFastQC Report
Tue 23 May 2023
EGAF00002207646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74769043
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC1272060.17013190873661443No Hit
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG1198600.16030698694378098No Hit
GTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCC1173890.15700214325332476No Hit
GCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAGTGTGGC1096720.14668102679875145No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACA1079230.1443418233934063No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT1069750.14307391897472863No Hit
GTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGG1014500.1356844971253678No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG960300.12843550772744275No Hit
AGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGC958300.1281680173437555No Hit
GGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCA951600.12727192455840314No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG930640.12446862533736054No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA908180.12146470832855251No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG899450.12029711280375757No Hit
GTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGA889980.11903054583699835No Hit
GAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATC886380.11854906314636125No Hit
GTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT875260.11706181661306003No Hit
GTGGAGTGTGGCGAGTCAGCTAAATACTTTGACGCCGGTGGGGATAGCGA861330.1151987460906782No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG858800.1148603707553138No Hit
GCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGG857100.11463300392917962No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT854690.11431067801683646No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC828270.11077713004832763No Hit
GGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGT824570.11028227283850617No Hit
AAATGAATGAGCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCG817760.10937146808205102No Hit
GTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAA785310.10503143660672506No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG785090.10500201266451946No Hit
GGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCG771420.10317371589201696No Hit
GTGACGATAACGTTGTAGATGTGGTCGTTACCTAGAAGGTTGCCTGGCTG768570.10279254209526262No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC768370.10276579305689387No Hit
GTTTGATTAGTCATTGTTGGGTGGTGATTAGTCGGTTGTTGATGAGATAT762850.10202751959791702No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG750420.10036506686330064No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)