FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22334886
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGA2290151.0253690124050778No Hit
TATTCT2181340.9766515038402255No Hit
CCGTCG2123290.9506607734644358No Hit
GCATAA2091360.9363647524325845No Hit
AGCCTA2034460.9108889116335762No Hit
TCAAGC1971440.8826729628259576No Hit
AAGCAT1955320.8754555541496831No Hit
AGTAGG1955210.8754063038423389No Hit
AATCGG1952100.8740138633346952No Hit
CAGCCT1941520.8692768792283068No Hit
GGTACC1924740.8617639687079666No Hit
CGTCAG1890380.8463799636138729No Hit
CTACCG1820980.8153074969802845No Hit
TGTGAT1808920.8099078723750817No Hit
GACTAA1800510.8061424625135764No Hit
TACTCG1793990.8032232624782594No Hit
CCGCTC1781590.797671409650356No Hit
TCCTCC1770280.792607582595228No Hit
CTATTA1757250.7867736598252618No Hit
TGGCAT1755850.7861468377317887No Hit
TAAGTC1750220.783626117455894No Hit
GAAGAT1724030.7719000670072818No Hit
CTCCGC1706580.764087177342208No Hit
AGTGCA1694710.7587726214496908No Hit
CTAGTT1693350.7581637085588886No Hit
GGATTC1642010.7351772469311014No Hit
TAATGA1641860.7351100874210864No Hit
AGACGG1606260.7191708970442026No Hit
AGTTCC1600200.7164576528395982No Hit
GGATCG1590270.7120116932766077No Hit
AGATGC1585970.7100864539895122No Hit
GGTGGT1571150.7034510944000342No Hit
ATTGCT1569190.7025735434691719No Hit
AAGTTG1562090.6993946599951305No Hit
TGTGTG1561430.6990991581510646No Hit
TCTTCA1551900.6948322906147808No Hit
CAAGCT1536240.687820837769219No Hit
AACTAG1515800.6786692352045136No Hit
GAGCCA1515150.6783782106611155No Hit
GTTACA1513020.6774245456189031No Hit
TTCCAT1508530.6754142376191219No Hit
CATAAC1506730.6746083234989425No Hit
AGTCAA1481080.6631240472863842No Hit
AATGTA1470080.6581990165519538No Hit
TATAAG1469100.6577602410865226No Hit
CGTACT1465220.656023048427469No Hit
CGCTTG1464630.6557588876880768No Hit
CTGTAT1455200.6515367931584697No Hit
GTACGC1453630.6508338569536464No Hit
GATCCT1451430.6498488508067602No Hit
ATACCT1449390.6489354814705569No Hit
CTATGC1433600.6418658237163154No Hit
GTCTCA1432380.6413195930348604No Hit
GTACTT1432290.6412792973288514No Hit
TGCGAC1431610.6409748408834502No Hit
TATTGG1403180.6282458750852814No Hit
CTTCAC1398960.6263564542035271No Hit
AACATA1391070.6228238639767403No Hit
TCGTGG1382590.6190271130105612No Hit
CCTCGG1377850.6169048724940884No Hit
GACTTG1370590.6136543522093643No Hit
CGAGAG1365400.6113306331628466No Hit
ACTAAT1360760.6092531656530505No Hit
CCGACT1356970.6075562687000059No Hit
TTAGCG1352850.6057116208249282No Hit
ATCAGC1339290.599640401119576No Hit
GAACCG1337170.5986912133780311No Hit
GTCGAG1336090.5982076649059234No Hit
GATTCA1330550.5957272403360375No Hit
TAGAGC1321270.5915723053164453No Hit
AGTTAG1319580.5908156415036101No Hit
TCAATG1318650.5903992525415174No Hit
TGCGGA1303750.583728074546698No Hit
ATGTGA1302500.58316841196324No Hit
TCTCTG1298890.5815521064222132No Hit
CATTAA1296520.5804909861639769No Hit
TTGAAT1284740.5752167259774686No Hit
TCGGAC1268960.5681515455238948No Hit
ACGTAA1266870.5672157896843529No Hit
GGCGTT1266850.5672068350830176No Hit
ACCTGC1257700.563110104972105No Hit
CCTTAT1257410.5629802632527429No Hit
TTCGTT1252970.5609923417563No Hit
ACGCTA1237870.5542316177481273No Hit
ACCGCT1237630.5541241625321034No Hit
AACAAT1232110.5516526925635529No Hit
TACGAA1226480.549131972287658No Hit
CGAATT1224410.5482051710494515No Hit
GAGCAC1211630.542483180796177No Hit
TGACAC1193670.5344419487970523No Hit
AGAGGT1193140.5342046518616661No Hit
GAGATA1192680.5339986960309535No Hit
CGAAGC1184210.5302064223654421No Hit
TCTCGC1177880.5273722910428108No Hit
AGAACG1175300.5262171474705535No Hit
ATTCAT1169950.5238217916133532No Hit
GCCTGG1164750.5214935952661679No Hit
ATCCAC1152410.5159686062422705No Hit
CTGTTC1150960.5153193976454592No Hit
CCATGA1147860.5139314344384834No Hit
GGACTA1131730.5067095484615413No Hit
GACCAT1122320.5024964085332695No Hit
TTCTGT1120230.5015606526937276No Hit
AGGTGT1119250.5011218772282966No Hit
GGCCAG1105780.4950909532289531No Hit
GCGTAT1105550.49498797531359684No Hit
CTCGTC1103630.4941283335854053No Hit
AGGACT1101090.49299109921581874No Hit
ACAGCC1101080.492986621915151No Hit
GCACAT1093630.4896510329177413No Hit
AGGTCA1091140.488536185051493No Hit
CCAGCG1086290.4863646942276759No Hit
ATTCGA1075320.4814530953952485No Hit
GGAGGC1074570.4811172978451737No Hit
GAATAC1073260.4805307714577097No Hit
TTACGT1067270.477848868357779No Hit
ACCAGG1064130.4764429959481325No Hit
CGTTGC1058070.473729751743528No Hit
TTAGTA1053880.47185376276377683No Hit
TATGGT1046380.46849578726302876No Hit
TACGGC1040730.4659661123857986No Hit
GTAAGA1037570.46455128537481677No Hit
AAGGCG1036120.46390207677800543No Hit
CAGTCC1026650.45966207304572765No Hit
CATGAG1025460.45912927426627564No Hit
CTGAGG987750.4422453734485146No Hit
CCACTT981010.43922767279850905No Hit
AATTGC968220.43350120524456676No Hit
TTCACA949470.42510626649269667No Hit
CATACG948860.4248331511519691No Hit
CACCTA942970.42219602105871507No Hit
ACGAAC929650.41623225656938656No Hit
CCTGAC923280.4133802160440846No Hit
GCTCAA913880.40917155341648037No Hit
CGTCTC912820.40869695954570795No Hit
TACAGT911870.4082716159822799No Hit
TTCTAC902720.40417488587136735No Hit
ATTGAA899930.40292571898508905No Hit
AGGCTT893780.40017217907447566No Hit
CAGTAG879900.393957685747758No Hit
ACACGA851650.3813093113616072No Hit
TGCATA849700.38043623773141266No Hit
GACACG847890.37962584631056545No Hit
CACATT841150.3766081456605599No Hit
GCGGCT839450.37584700454705705No Hit
CCTGCT837460.3749560217141919No Hit
TCCACG835570.3741098118880034No Hit
GAGAAG824090.3689698707215251No Hit
CGTGAA817160.3658671013588339No Hit
GCTTCT806350.3610271393370891No Hit
GTGGTG800600.35845269145318226No Hit
TGATAA770460.34495810724084286No Hit
AACTCT765900.3429164581363881No Hit
CGACAA753120.33719446788311347No Hit
CAACGG744910.33351860403496125No Hit
GAGTGA742770.33256046169208114No Hit
AAGAGG734790.3289875757592853No Hit
ATCCGG721760.32315365298931903No Hit
AACCTT720630.322647718013873No Hit
GGCAGG720230.32246862598716647No Hit
CCGGAA716230.32067770572010085No Hit
TCCAGA712630.3190658774797418No Hit
CGAGCC710720.31821071305221793No Hit
GGTAAT698700.3128289976496858No Hit
GGCCGT695320.31131567002401533No Hit
CTGGAG689960.30891583686614743No Hit
ATGCCG676040.3026834343367591No Hit
CAGGAC670600.30024778277354985No Hit
CAAGGA666850.29856879502317585No Hit
TCATGT663490.29706442199884076No Hit
GCCATA652270.2920408906497217No Hit
CTTAGC647540.28992312743391657No Hit
AAGCGC646050.28925600963443465No Hit
GCGCAG643330.2880381838528301No Hit
ATGGCC617580.27650913463359517No Hit
GTCTGC595160.2664710265366924No Hit
GTTGGC448410.20076663923872276No Hit
GCTGTC433600.19413575694991236No Hit
GAGTCG346550.15516085463789697No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)