FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22312604
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGA2317051.038448941235187No Hit
TATTCT2214150.9923315091326858No Hit
GCATAA2098610.9405491174405282No Hit
AGCCTA2061830.9240651606598674No Hit
CCGTCG2042820.9155453124162469No Hit
AAGCAT2002160.8973224281666093No Hit
TCAAGC1993520.8934501773078571No Hit
AGTAGG1966870.8815062553882101No Hit
AATCGG1958610.8778043118588938No Hit
CAGCCT1944810.8716194667372754No Hit
GGTACC1911040.8564845232766198No Hit
CGTCAG1906820.8545932155655164No Hit
CTATTA1782650.7989430547864338No Hit
TAAGTC1779120.7973609893314112No Hit
CTACCG1759280.7884691540261279No Hit
GACTAA1757110.7874966095396128No Hit
TGGCAT1744840.7819974755075652No Hit
TCCTCC1741140.780339219931479No Hit
TACTCG1739520.7796131728954631No Hit
CCGCTC1738560.7791829228000462No Hit
TGTGAT1727690.7743112368238149No Hit
AGTGCA1716060.769098936188712No Hit
GAAGAT1711330.7669790581144182No Hit
TAATGA1682140.7538967661506474No Hit
CTCCGC1673290.7499303980835227No Hit
CTAGTT1665400.7463942801118149No Hit
GGATTC1634610.7325949046556826No Hit
AGACGG1607450.7204224123728454No Hit
AAGTTG1580190.7082051023717357No Hit
GGATCG1579300.7078062246791096No Hit
GGTGGT1572880.704928927166009No Hit
TGTGTG1568230.7028449032663332No Hit
AGTTCC1563550.7007474340511757No Hit
TTCCAT1550160.6947463415744751No Hit
AGATGC1544890.6923844478215093No Hit
ATTGCT1542630.6913715673885487No Hit
GTTACA1537200.6889379652863467No Hit
CAAGCT1534730.6878309676450135No Hit
GAGCCA1523740.6829055004068553No Hit
CATAAC1500730.6725929434323309No Hit
AACTAG1499950.6722433652298047No Hit
AATGTA1495650.670316203344083No Hit
TCTTCA1489540.6675778407576274No Hit
AGTCAA1488670.6671879266086558No Hit
GTACGC1479450.6630557329839224No Hit
GATCCT1468420.6581123386584551No Hit
CTATGC1467970.6579106589262285No Hit
CGCTTG1426010.6391051443390471No Hit
TATAAG1424220.6383029071819676No Hit
ATACCT1421830.6372317637152526No Hit
CGTACT1419790.6363174822624916No Hit
TATTGG1416180.6346995626328509No Hit
CTGTAT1398900.6269550609153464No Hit
CCGACT1396570.6259108080795949No Hit
TGCGAC1394540.6250010083986611No Hit
CTTCAC1387340.6217741326830342No Hit
CGAGAG1383670.62012932242243No Hit
GTCTCA1378310.6177270927230188No Hit
GTACTT1370840.6143792091680559No Hit
GAACCG1353330.6065316266985243No Hit
CCTCGG1352820.606303056335334No Hit
AACATA1335650.5986078541079293No Hit
CATTAA1334840.5982448305899213No Hit
TCTCTG1331880.5969182261290524No Hit
TCGTGG1327470.5949417647532309No Hit
GACTTG1321980.5924812720200654No Hit
ACTAAT1317300.590383802804908No Hit
ATCAGC1312040.5880263908237694No Hit
TTAGCG1311870.587950200702706No Hit
TCGGAC1310820.5874796146608438No Hit
GATTCA1304380.5845933536040886No Hit
TCAATG1296760.5811782434717168No Hit
GGCGTT1293030.5795065425801489No Hit
ACGTAA1292740.5793765711971584No Hit
TTCGTT1291650.578888058067987No Hit
TTGAAT1281000.5741149710719555No Hit
TAGAGC1274540.5712197464715458No Hit
ATGTGA1272020.5700903399710765No Hit
ACGCTA1271830.5700051863063585No Hit
AGTTAG1267790.5681945504881456No Hit
TACGAA1263720.5663704693544509No Hit
ACCGCT1263150.5661150083602972No Hit
AACAAT1261080.5651872815920544No Hit
CCTTAT1259970.5646898049192286No Hit
TGCGGA1259120.5643088543139115No Hit
GTCGAG1256260.5630270675713153No Hit
ACCTGC1250470.560432121683332No Hit
CGAATT1226320.5496086427205No Hit
GAGCAC1199490.5375840489079625No Hit
GAGATA1188860.5328199254555855No Hit
TGACAC1176940.5274776534374921No Hit
AGAGGT1176660.5273521638263289No Hit
ATCCAC1165260.5222429439432529No Hit
CGAAGC1161540.5205757248235122No Hit
AGAACG1158770.5193342740273614No Hit
TCTCGC1142750.5121544755600915No Hit
CTGTTC1142450.5120200224052738No Hit
CTCGTC1140850.5113029389129122No Hit
GCCTGG1133370.507950573586122No Hit
ATTCAT1125420.5043875649834506No Hit
GGACTA1113080.4988570585486123No Hit
ACAGCC1109950.49745426396667997No Hit
CCATGA1107410.49631589392255604No Hit
GACCAT1107180.49621281317052907No Hit
TTCTGT1102140.49395400016959023No Hit
AGGACT1098500.49232263522446773No Hit
AGGTGT1094690.4906150801582818No Hit
TATGGT1083550.48562238634271465No Hit
GCGTAT1075470.48200111470628887No Hit
AGGTCA1073380.4810644243943917No Hit
GGCCAG1073280.48101960667611904No Hit
GGAGGC1071380.4801680700289397No Hit
GAATAC1066830.4781288638475366No Hit
TTACGT1062440.4761613660153696No Hit
ATTCGA1060350.4752246757034724No Hit
GCACAT1059390.4747944256080554No Hit
CGTTGC1057610.4739966702228032No Hit
CCAGCG1045690.4686543982047098No Hit
ACCAGG1020060.45716761701144343No Hit
GTAAGA1017910.45620403606858256No Hit
TTAGTA1013550.4542499835518974No Hit
AAGGCG1013530.4542410200082429No Hit
CAGTCC1009070.4522421497732851No Hit
TACGGC996260.4465010000625655No Hit
CATGAG985690.4417637672411522No Hit
AATTGC961030.43071171791512997No Hit
CATACG960290.43038006679991275No Hit
CTGAGG955040.42802713659060143No Hit
CCACTT952950.4270904462787042No Hit
CACCTA944500.4233033490846698No Hit
TTCACA940310.421425486689048No Hit
ACGAAC933680.4184540719675749No Hit
CCTGAC922700.4135330865012439No Hit
CGTCTC904090.40519250913071375No Hit
TACAGT886680.3973897443794548No Hit
AGGCTT879680.39425250410037305No Hit
TTCTAC875890.39255391257784167No Hit
GCTCAA875800.39251357663139635No Hit
CAGTAG871440.3905595241147111No Hit
ATTGAA863380.38694721602193993No Hit
ACACGA846460.3793640580902166No Hit
TGCATA842960.37779543795067577No Hit
CACATT842900.3777685473197122No Hit
TCCACG823300.3689842745382834No Hit
CCTGCT818710.3669271412695712No Hit
GAGAAG812940.36434115892524244No Hit
CGTGAA810740.36335516912324534No Hit
GCGGCT809420.36276357524204705No Hit
GACACG808680.36243192412682984No Hit
GCTTCT798310.35778432674196164No Hit
CGACAA781310.35016531463562034No Hit
GTGGTG761280.3411883256656193No Hit
GAGTGA748320.3353799493774909No Hit
AACTCT741550.3323457898504361No Hit
CAACGG735740.32974188041879826No Hit
TGATAA734230.3290651328728821No Hit
AACCTT717430.32153575620308594No Hit
GGCAGG711710.3189721827178934No Hit
ATCCGG710750.31854193262247654No Hit
CGAGCC709320.3179010392511784No Hit
AAGAGG704450.31571841637130293No Hit
CCGGAA693770.3109318840597897No Hit
TCCAGA691020.3096993968072933No Hit
CTGGAG681910.3056165026726598No Hit
CAGGAC678240.3039716924120555No Hit
GGTAAT672820.30154257208168084No Hit
ATGCCG660860.2961823729762783No Hit
GGCCGT651820.29213085124443566No Hit
CAAGGA643700.28849165252070086No Hit
TCATGT640220.28693199592481455No Hit
GCCATA630050.28237403397649147No Hit
CTTAGC628580.2817152135178843No Hit
AAGCGC625740.2804423903189426No Hit
ATGGCC613580.2749925557769949No Hit
GCGCAG612680.2745891963125416No Hit
GTCTGC565420.2534083426569127No Hit
GTTGGC425780.1908248808610595No Hit
GCTGTC417800.18724842694290636No Hit
GAGTCG342340.15342897673440536No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)