FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24784239
Sequences flagged as poor quality0
Sequence length6
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAA3662761.477858569714406No Hit
CCGCTC3625711.4629095531236604No Hit
TCCTCC3622891.4617717332374014No Hit
TACTCG3504331.413934880147016No Hit
CTCCGC3354541.353497277039654No Hit
AGATGC3265691.317647880977907No Hit
CAAGCT3123071.2601032454536933No Hit
CATAAC3049611.2304634408988713No Hit
TCGTGG2992211.207303560944518No Hit
AACATA2905711.1724023481213202No Hit
TGTGAT2876571.1606448759633088No Hit
GTCGAG2685081.0833820638995613No Hit
TGCGGA2594561.0468588525151004No Hit
ACCTGC2535631.0230816447501172No Hit
CCGTCG2517711.0158512432033922No Hit
CCATGA2487481.0036539754155855No Hit
CTACCG2450190.9886081230898396No Hit
GCACAT2382870.9614456994221205No Hit
ATTGCT2355630.9504548434995321No Hit
AGAGGT2309720.9319309743583412No Hit
TCTCGC2286080.9223926544607643No Hit
TTAGTA2279400.9196973931699094No Hit
ACCAGG2248520.9072378619331422No Hit
CGCTTG2218350.8950648030790858No Hit
GCGTAT2190900.8839892158883718No Hit
CTAGTT2173020.8767749536308135No Hit
TCTTCA2171570.8761899043985172No Hit
CATGAG2150230.867579593628031No Hit
AGGTGT2130630.8596713419363007No Hit
CGTACT2069760.8351113786467279No Hit
GATTCA2012530.8120200906713335No Hit
CTGTAT1943180.7840385980783997No Hit
TATAAG1892080.763420656167817No Hit
TGCGAC1889710.7624644032846842No Hit
ATACCT1868430.7538783014479484No Hit
CACCTA1846500.7450299361622521No Hit
AGTGCA1845250.7445255833757898No Hit
ACTAAT1826770.7370692317807297No Hit
CCTGCT1814020.7319248333588132No Hit
GCCTGG1778480.7175850749341144No Hit
AGGCTT1776160.7166489961624402No Hit
GACACG1770110.7142079286759622No Hit
CGTCTC1744120.7037214255398361No Hit
ATGTGA1728550.6974392072316604No Hit
GACCGA1715780.6922867391651605No Hit
CAGCCT1710430.6901281092391015No Hit
ATCAGC1706660.688606981235131No Hit
GGTACC1688400.6812393957304882No Hit
GCGGCT1661180.6702566094524831No Hit
TTCCAT1649080.6653744744795271No Hit
GACTTG1618270.6529431869988019No Hit
ATTCGA1597500.6445628610989428No Hit
CTGAGG1585500.6397210743489037No Hit
CCACTT1574000.6350810287134497No Hit
AAGAGG1573830.6350124367344908No Hit
ATTCAT1572240.6343708999901106No Hit
AGAACG1542730.6224641394073064No Hit
AGTTAG1532680.6184091430041487No Hit
CCAGCG1524810.6152337378605816No Hit
CGTCAG1515190.6113522388159669No Hit
GAAGAT1493700.6026814057111054No Hit
AGTTCC1466250.5916058185203912No Hit
GAACCG1465780.5914161818726812No Hit
TTCTAC1452360.5860014503572211No Hit
TCCAGA1442420.581990836999272No Hit
GACCAT1440740.5813129868542665No Hit
AGGTCA1420840.5732836904937852No Hit
GGTAAT1417030.5717464232006477No Hit
CGAAGC1415550.5711492695014763No Hit
TTCTGT1405450.5670740989868601No Hit
GGCCGT1398750.5643707680514217No Hit
AATTGC1392830.5619821532547359No Hit
GTCTCA1392740.5619458398541105No Hit
TCGGAC1375140.55484455262072No Hit
GTACTT1372710.553864090803837No Hit
CTATTA1370360.5529159075652877No Hit
CTTAGC1319820.5325239157030401No Hit
TACGAA1319490.5323907665674141No Hit
AAGCGC1317890.5317451950007421No Hit
GCTCAA1282580.5174982374887525No Hit
CTTCAC1282430.517437715154377No Hit
TAGAGC1281810.5171875561722916No Hit
GGATCG1274140.5140928474745583No Hit
GTGGTG1270590.5126604855610052No Hit
TTCACA1233850.4978365484613024No Hit
TTAGCG1227830.49540758544169944No Hit
GCATAA1223770.49376944759126956No Hit
ATTGAA1201660.48484845550432276No Hit
CAGTCC1201300.48470320190182153No Hit
GAGCCA1194270.481866721830757No Hit
AAGCAT1138820.45949363222328515No Hit
GATCCT1135720.4582428373128584No Hit
TCAAGC1131990.4567378485980546No Hit
AGTAGG1112700.44895467639736686No Hit
GTACGC1110630.44811946818298515No Hit
AAGTTG1106090.4462876588625537No Hit
TTGAAT1105290.4459648730792178No Hit
TGTGTG1091030.4402112164912548No Hit
TATTGG1081900.43652742373893344No Hit
TCAATG1080870.4361118370428884No Hit
AGCCTA1079120.4354057431418411No Hit
TGACAC1072350.4326741684503608No Hit
TGGCAT1067620.430765697506387No Hit
CGAGAG1058410.4270496261757321No Hit
TCATGT1049760.4235595048934123No Hit
AATGTA1041830.42035989081609487No Hit
TGATAA1019940.4115276648195654No Hit
TATTCT1016090.4099742582372612No Hit
CCGGAA1009530.40732741481390655No Hit
GAGCAC1003930.40506791433055506No Hit
TAAGTC992700.40053680889697685No Hit
TCTCTG959980.38733487035853714No Hit
AACTAG938310.37859141045242506No Hit
GTTGGC936020.37766743614762593No Hit
ACGCTA930280.3753514481521906No Hit
TAATGA925060.37324527091592363No Hit
AATCGG922240.37210745102966447No Hit
GCTGTC921390.37176449113487003No Hit
TTACGT916780.3699044380583967No Hit
AGGACT910700.36745126610504364No Hit
CATTAA901350.3636787072623049No Hit
AAGGCG899040.3627466633129224No Hit
GCGCAG891860.3598496609074824No Hit
TTCGTT887420.35805819980996795No Hit
TACAGT878700.3545398347716062No Hit
CGTTGC870810.3513563599834556No Hit
CTCGTC867900.3501822266965712No Hit
CCTCGG861640.34765642794196744No Hit
AGACGG858110.34623213567299765No Hit
GTTACA844590.3407770559346204No Hit
GGTGGT835320.3370367756702153No Hit
CCTGAC819500.33065368680474716No Hit
CTATGC804980.32479512483719997No Hit
GGAGGC803990.32439567743032177No Hit
CGACAA800290.32290279318239307No Hit
GTCTGC788690.318222399324022No Hit
GCCATA761430.30722347375685005No Hit
GGATTC746080.3010300215390918No Hit
CTGTTC741850.29932329170970307No Hit
AACTCT734750.29645856788259667No Hit
ACACGA732910.29571616058092404No Hit
CCTTAT731430.2951190068817526No Hit
TCCACG718350.28984145932421No Hit
CGTGAA705640.2847132001914604No Hit
GAGAAG701760.2831476891422811No Hit
ACGTAA700020.28244563006352547No Hit
CCGACT697680.28150148164726785No Hit
ACAGCC689130.27805170858786504No Hit
AGTCAA667890.269481746040296No Hit
GCTTCT663650.26777098138861555No Hit
TACGGC658240.2655881425288063No Hit
CATACG648340.26159366846002413No Hit
GGCGTT643760.25974571985042594No Hit
CAAGGA639980.25822055702416363No Hit
GGCCAG636980.2570101103366539No Hit
AACAAT632330.25513391797101376No Hit
GAATAC623300.2514904734416094No Hit
GGCAGG620020.2501670517299321No Hit
CGAATT608200.24539789178114366No Hit
CGAGCC606560.244736180925305No Hit
ACGAAC593010.2392689967200526No Hit
GAGATA591180.23853062424067165No Hit
CAGGAC581940.23480244844314163No Hit
GGACTA563210.22724522629078905No Hit
ACCGCT541200.21836458242675919No Hit
TGCATA537780.21698467320299808No Hit
TATGGT530200.2139262779058901No Hit
ATCCAC522650.21087998707565725No Hit
GTAAGA511680.20645378702166323No Hit
CAGTAG508440.2051465045991527No Hit
ATCCGG457500.18459311984523713No Hit
AACCTT457120.18443979659815257No Hit
CAACGG454710.18346740442585305No Hit
CACATT421690.17014442121866238No Hit
GAGTGA404370.16315610900943944No Hit
ATGCCG385650.1556029216793786No Hit
ATGGCC359360.14499537387450145No Hit
CTGGAG337570.13620349610088894No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)