FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25824325
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCGA2692121.0424744886846027No Hit
TATTCT2546560.9861090270510459No Hit
GCATAA2441650.9454845383180394No Hit
AGCCTA2396940.9281714042864625No Hit
CCGTCG2384680.9234239423489287No Hit
AAGCAT2306500.8931501597815238No Hit
TCAAGC2281530.8834809815939043No Hit
AATCGG2274210.8806464447763882No Hit
AGTAGG2263960.8766773187682544No Hit
CAGCCT2263310.8764256180945679No Hit
GGTACC2248330.8706248856456075No Hit
CGTCAG2214160.857393174845809No Hit
CTATTA2059870.7976471795487393No Hit
TGGCAT2054790.7956800419759278No Hit
GACTAA2047980.7930429933793042No Hit
CTACCG2040600.7901852226534478No Hit
TAAGTC2040490.790142627154824No Hit
TCCTCC2038960.7895501624921465No Hit
CCGCTC2026050.7845509998809261No Hit
TACTCG2023440.7835403248681233No Hit
TGTGAT1999620.7743164632570261No Hit
GAAGAT1991350.7711140562241221No Hit
AGTGCA1979930.7666918690033524No Hit
CTCCGC1945790.7534717751577243No Hit
TAATGA1937800.7503777930304083No Hit
CTAGTT1920480.7436709381561764No Hit
GGATTC1907550.7386640309088427No Hit
AGACGG1881040.7283985157404889No Hit
GGATCG1844990.714438809146028No Hit
GGTGGT1828680.7081230583955244No Hit
AGTTCC1822190.7056099239767157No Hit
TGTGTG1816750.7035033829538623No Hit
AAGTTG1810190.7009631423086566No Hit
AGATGC1803210.698260264305069No Hit
TTCCAT1779150.6889434670606105No Hit
GAGCCA1776940.6880876847700763No Hit
ATTGCT1774590.6871776900267481No Hit
GTTACA1773370.6867052672238287No Hit
CAAGCT1765510.68366162523125No Hit
TCTTCA1737710.6728965810335797No Hit
AACTAG1735160.6719091399291172No Hit
CATAAC1727760.6690436245671475No Hit
AGTCAA1726630.6686060526267386No Hit
GTACGC1713520.663529443654384No Hit
AATGTA1711470.6627356184527572No Hit
GATCCT1711330.6626814059999632No Hit
CTATGC1692810.6555098729589254No Hit
CGCTTG1656000.6412558701921541No Hit
CGTACT1653980.6404736619446975No Hit
TATAAG1652530.6399121758264738No Hit
ATACCT1647460.6379489105717187No Hit
TATTGG1631970.6319506899018658No Hit
CTGTAT1623990.6288605800926065No Hit
GTCTCA1609040.6230714645978161No Hit
TGCGAC1608490.6228584871046968No Hit
CTTCAC1608470.6228507424685834No Hit
CCGACT1600990.6199542485621599No Hit
GTACTT1600070.6195979953009421No Hit
CGAGAG1595850.617963877081008No Hit
CCTCGG1588740.6152106589426829No Hit
GAACCG1568520.6073808318320033No Hit
AACATA1554170.601824055420616No Hit
TCGTGG1542510.5973089325664853No Hit
CATTAA1541840.5970494872566853No Hit
GACTTG1540080.5963679592787033No Hit
TTAGCG1528830.592011601464898No Hit
ATCAGC1526820.591233265535498No Hit
ACTAAT1521570.5892002985557222No Hit
TCTCTG1515630.5869001416300329No Hit
TCAATG1511720.585386065269857No Hit
GATTCA1507650.5838100318207736No Hit
GGCGTT1497710.579960947672398No Hit
TCGGAC1495750.5792019733332817No Hit
TAGAGC1490010.5769792627687268No Hit
ACGTAA1486190.5755000372710612No Hit
TTCGTT1483500.5744583837138048No Hit
GTCGAG1478970.5727042236341124No Hit
TTGAAT1475850.571496060400417No Hit
TGCGGA1475540.5713760185406589No Hit
ATGTGA1471010.5696218584609666No Hit
ACGCTA1467450.5682433132327757No Hit
AGTTAG1466760.5679761232868623No Hit
CCTTAT1462120.5661793677085462No Hit
AACAAT1456670.5640689543676359No Hit
ACCGCT1453350.5627833447728062No Hit
ACCTGC1450620.5617262019433228No Hit
TACGAA1445800.5598597446399858No Hit
CGAATT1420870.5502060557245929No Hit
GAGCAC1416630.5485641928685454No Hit
GAGATA1381210.5348484423116577No Hit
TGACAC1363620.5280370348498944No Hit
CGAAGC1356780.5253883692991007No Hit
AGAGGT1349630.5226196618885489No Hit
ATCCAC1346740.521500561970158No Hit
CTGTTC1335810.5172681183341675No Hit
AGAACG1335140.5170086730243675No Hit
TCTCGC1328200.5143212842930067No Hit
GCCTGG1321840.5118584900089354No Hit
ATTCAT1314780.509124633460894No Hit
CTCGTC1313020.5084431054829119No Hit
GGACTA1310690.5075408553756972No Hit
GACCAT1298020.5026346283978381No Hit
CCATGA1293930.5010508503126412No Hit
AGGACT1280770.49595487975000313No Hit
ACAGCC1280750.49594713511388977No Hit
GGAGGC1275790.49402646535775857No Hit
GGCCAG1270440.49195477519741565No Hit
TTCTGT1266340.4903671247941621No Hit
AGGTGT1259800.48783462878506983No Hit
GCGTAT1251960.48479873142860463No Hit
GAATAC1241200.48063211719957827No Hit
AGGTCA1237600.4792380826991606No Hit
CGTTGC1230630.47653907701362963No Hit
TATGGT1230510.476492609196949No Hit
GCACAT1229940.4762718870677162No Hit
TTACGT1224140.474025942594821No Hit
ATTCGA1222370.47334054229878225No Hit
CCAGCG1219970.47241118596517045No Hit
ACCAGG1198920.46425995645578344No Hit
TACGGC1198400.4640585959168342No Hit
GTAAGA1186730.4595396007446468No Hit
AAGGCG1184370.4586257336832618No Hit
CATACG1177600.45600417435886514No Hit
TTAGTA1174660.4548657128501906No Hit
CAGTCC1170750.4533516364900147No Hit
CATGAG1148090.44457696377349654No Hit
CCACTT1112090.43063661876931925No Hit
AATTGC1105620.42813122898662404No Hit
CTGAGG1104100.4275426366420032No Hit
CACCTA1085420.4203091465120579No Hit
TTCACA1083110.4194146410409565No Hit
ACGAAC1080090.41824520098782836No Hit
CCTGAC1064230.4121037045498769No Hit
CGTCTC1049650.40645786482318513No Hit
GCTCAA1033500.40020407116158896No Hit
TACAGT1026140.397354045071846No Hit
TTCTAC1020640.3952242701406523No Hit
AGGCTT1015990.3934236422442794No Hit
CAGTAG1010680.39136744135616325No Hit
ATTGAA998720.386736148960331No Hit
ACACGA990400.38351438033714336No Hit
CACATT985090.3814581794490272No Hit
TGCATA982610.3804978445709617No Hit
TCCACG965640.3739265208287148No Hit
CCTGCT955160.3698683315052765No Hit
GACACG952620.36888476271887066No Hit
GCGGCT952120.3686911468160349No Hit
CGTGAA949000.3674829835823395No Hit
GAGAAG944600.3657791636373845No Hit
GCTTCT933070.36131438091799106No Hit
CGACAA897160.34740888677632425No Hit
GTGGTG891860.3453565582062648No Hit
GAGTGA870260.3369923512037585No Hit
AACTCT867940.3360939734146004No Hit
CAACGG864300.3346844496419558No Hit
TGATAA852380.33006864651835044No Hit
AACCTT833690.3228312840703484No Hit
ATCCGG826080.3198844500291876No Hit
GGCAGG823760.3189860722400295No Hit
CGAGCC822030.3183161612162177No Hit
AAGAGG817370.316511661001788No Hit
CCGGAA808050.3129026605729288No Hit
TCCAGA804730.31161705097809916No Hit
CTGGAG790770.3062112949709237No Hit
CAGGAC786760.30465849543018064No Hit
GGTAAT783430.3033690135172943No Hit
ATGCCG780350.30217633955582573No Hit
GGCCGT777940.30124311090415723No Hit
CAAGGA747560.2894790086478543No Hit
TCATGT743620.2879533153335082No Hit
GCCATA741450.2871130223152009No Hit
AAGCGC729630.2825359423721627No Hit
CTTAGC729530.28249721919159554No Hit
GCGCAG719270.278524220865405No Hit
ATGGCC716680.27752129048871554No Hit
GTCTGC661210.25604154222811243No Hit
GTTGGC505110.19559465736277717No Hit
GCTGTC490520.18994494531802864No Hit
GAGTCG401460.1554580807049168No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)