FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002208414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002208414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21305044
Sequences flagged as poor quality0
Sequence length6
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACC3261271.530750182914431No Hit
ACCAGG2909311.3655498669704695No Hit
GTCTCA2711841.2728628957537003No Hit
AACATA2276621.0685826323569199No Hit
CAGGAC2211311.0379279197921394No Hit
TCTTCA1973890.9264895205097909No Hit
GAAGAT1947590.9141450259384586No Hit
CGAGCC1926190.9041004562112145No Hit
GCTGTC1866130.8759099488365291No Hit
AACCTT1783900.8373134549733856No Hit
CATGAG1780860.8358865628252163No Hit
TGCATA1717550.8061705950947579No Hit
CAGCCT1710640.8029272316921758No Hit
GGCCGT1701960.7988530791112188No Hit
CGTACT1700860.798336769452342No Hit
CAACGG1700680.798252282417253No Hit
GCACAT1683020.7899631655301909No Hit
GTAAGA1666400.7821621959569761No Hit
TGTGAT1636540.7681467355805507No Hit
AAGCAT1633400.7666729061906655No Hit
TAAGTC1565860.7349714931356162No Hit
AGTCAA1539350.7225284303566799No Hit
AGTTAG1528580.7174732894238567No Hit
TTCCAT1528270.7173277839745368No Hit
CGTCAG1509760.7086397005328879No Hit
TGATAA1496810.7025613277306538No Hit
CTGGAG1496460.7023970473846475No Hit
AGATGC1494070.7012752473076329No Hit
CATAAC1493400.700960767788135No Hit
AACAAT1464360.6873301927937816No Hit
AGTGCA1455770.6832982837303692No Hit
TTGAAT1455110.682988497935043No Hit
ATGTGA1452810.6819089413755729No Hit
TTACGT1412190.6628430337904958No Hit
CCAGCG1406770.6602990352894835No Hit
ACACGA1405620.6597592570097485No Hit
CTGTTC1401640.6578911547894479No Hit
GAGATA1391610.6531833494453239No Hit
GACACG1390790.6527984640632519No Hit
TATTCT1385360.650249771838068No Hit
TACGGC1372010.6439836500689695No Hit
CCGTCG1368550.6423596215055927No Hit
ATCCAC1367920.6420639168827813No Hit
CAGTCC1365830.641082928530915No Hit
AATCGG1359100.637924052163422No Hit
CCTTAT1358260.6375297793330068No Hit
GTGGTG1339960.6289402640989618No Hit
TATAAG1318520.6188769194750313No Hit
GTTACA1316480.6179193997440231No Hit
ATTGCT1289520.6052651193773644No Hit
GCTCAA1288310.6046971787525997No Hit
ATTCAT1285400.6033313050186613No Hit
GTCTGC1266500.5944601663343198No Hit
GCTTCT1256310.5896772614034498No Hit
TATGGT1253700.5884521993946598No Hit
CCACTT1253120.5881799633927065No Hit
GGACTA1248240.585889425996961No Hit
GCCATA1248170.5858565699277598No Hit
CTGTAT1247080.5853449539930544No Hit
CCTCGG1239650.5818575169335487No Hit
CTTAGC1235560.5799377837473605No Hit
TGCGAC1234930.5796420791245491No Hit
CAAGGA1230710.5776613275241299No Hit
AAGCGC1230320.5774782722814372No Hit
CCGACT1227290.5760560738574396No Hit
GCATAA1220940.5730755590084677No Hit
TCATGT1213360.5695177160863878No Hit
GAGCAC1211490.5686399896662969No Hit
TTCTGT1197390.5620218385843277No Hit
AGGACT1195380.5610784000258342No Hit
ATCCGG1192990.5599565999488196No Hit
GAGTGA1187930.5575815755179853No Hit
CCATGA1186570.5569432290306464No Hit
ATGCCG1181400.5545165736339244No Hit
GACCAT1180770.554220869011113No Hit
GACTAA1174180.5511277047820226No Hit
AAGTTG1169350.5488606360071352No Hit
ACAGCC1169030.5487104368336437No Hit
TCCACG1163490.5461101136425722No Hit
TAATGA1154240.5417684187838335No Hit
AATTGC1140110.5351361865293496No Hit
CGTGAA1137910.5341035672115955No Hit
GTACTT1136710.5335403203110024No Hit
CGAATT1134510.5325077009932484No Hit
GCGTAT1129970.5303767502193377No Hit
AGTTCC1107770.519956682558365No Hit
ACCGCT1100650.5166147509481792No Hit
GAGAAG1091050.5121087757434343No Hit
AGCCTA1090800.511991432639144No Hit
CCTGCT1088250.5107945329753837No Hit
GGCGTT1087130.5102688358681634No Hit
GGTGGT1083720.5086682759256447No Hit
AGAACG1077930.5059506096302828No Hit
TACAGT1074430.5043078061702195No Hit
TTCACA1066020.5003603841418962No Hit
AGGTGT1065750.5002336535892626No Hit
TCTCTG1064260.49953428868769295No Hit
GAATAC1063410.4991353221331062No Hit
TACGAA1062530.4987222744060045No Hit
CACCTA1054740.49506586327632085No Hit
TTCTAC1053990.49471383396345014No Hit
AGTAGG1052080.49381733264667277No Hit
TCCTCC1048560.4921651417382663No Hit
GCGCAG1027870.4824538264272067No Hit
TCAAGC1023490.4803979752400418No Hit
GGAGGC1021300.4793700496464593No Hit
GTCGAG1018660.47813090646515444No Hit
CGACAA1017340.477511334874502No Hit
ATTGAA1016910.47730950473512285No Hit
ACGAAC1016550.47714053066494494No Hit
TACTCG1014460.4761595423130785No Hit
TTAGTA1011720.4748734618900576No Hit
CTACCG1009960.4740473664358543No Hit
ACGTAA1003030.47079461558492913No Hit
GACTTG1002290.47044727999623No Hit
ACTAGC1001110.4698934205439801No Hit
CAGTAG990570.46494623526710394No Hit
AGACGG990470.4648992980253878No Hit
GTACGC983340.46155267269103034No Hit
CTTCAC977760.45893357460327233No Hit
ACTAAT966220.4535170169092352No Hit
TGTGTG955370.4484243261830391No Hit
GAGTCG952820.4472274265192787No Hit
CTAGTT949130.4454954422999549No Hit
CACATT949070.4454672799549252No Hit
GGCCAG947990.44496035774439147No Hit
TAGAGC932910.4378822216936046No Hit
GGATTC921930.4327285125531775No Hit
TCAATG901890.4233222893132725No Hit
TGCGGA898780.42186254109590193No Hit
GTTGGC892680.4189993693512203No Hit
ATTCGA889220.41737534078784344No Hit
AGGTCA882640.41428687028292455No Hit
AGGCTT881870.4139254535217106No Hit
AAGGCG875770.4110622817770289No Hit
GAACCG875410.410893307706851No Hit
CAAGCT868640.4077156564426715No Hit
TCGTGG865590.40628407057033067No Hit
CGAAGC865110.40605877181009337No Hit
GACCGA858490.4029515264084881No Hit
TCTCGC855360.4014823907427743No Hit
CTCCGC854450.40105526184315793No Hit
TGACAC848870.39843616375539986No Hit
CGTTGC841860.3951458631111017No Hit
ATGGCC841420.3949393392475509No Hit
CTCGTC841120.3947985275224027No Hit
ATACCT819910.3848431385544193No Hit
CCTGAC813520.381843848808761No Hit
CGCTTG791540.3715270430795637No Hit
CCGGAA775110.36381525426560957No Hit
GGCAGG774380.36347261240108214No Hit
CATACG771440.362092657494629No Hit
ACCTGC767020.36001803141077765No Hit
TTCGTT760810.3571032287002083No Hit
GGTAAT755700.3547047356485159No Hit
AAGAGG754010.3539114962635139No Hit
ATCAGC752030.35298213887753527No Hit
ACGCTA747150.35069160148178996No Hit
TCCAGA741450.3480161787039726No Hit
TGGCAT740160.34741068828583505No Hit
TTAGCG710680.33357358942793075No Hit
CTATTA710030.33326849735677616No Hit
TATTGG698280.3277533714551352No Hit
AACTCT698190.3277111279375908No Hit
CCGCTC691430.32453817039758287No Hit
CATTAA682910.3205391174033717No Hit
TCGGAC672700.3157468250241586No Hit
CGTCTC662070.3107573962297379No Hit
AACTAG652620.3063218268875671No Hit
GCCTGG643120.3018627889245383No Hit
CTGAGG641770.301229136161371No Hit
AATGTA617090.2896450249058392No Hit
GATTCA614990.2886593428298012No Hit
AGAGGT597870.2806236870480061No Hit
CTATGC585960.2750334615596194No Hit
GCGGCT566320.2658149872865787No Hit
CGAGAG559590.26265611091908564No Hit
GATCCT549290.25782157502232805No Hit
GGATCG545270.25593469790534107No Hit
GAGCCA453670.21294018449339977No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)